Abstract:
:Specific nucleic acid detection in vitro or in vivo has become increasingly important in the discovery of genetic diseases, diagnosing pathogen infection and monitoring disease treatment. One challenge, however, is that the amount of target nucleic acid in specimens is limited. Furthermore, direct sensing methods are also unable to provide sufficient sensitivity and specificity. Fortunately, due to advances in nanotechnology and nanomaterials, nanotechnology-based bioassays have emerged as powerful and promising approaches providing ultra-high sensitivity and specificity in nucleic acid detection. This chapter presents an overview of strategies used in the development and integration of nanotechnology for nucleic acid detection, including optical and electrical detection methods, and nucleic acid assistant recycling amplification strategies. Recent 5 years representative examples are reviewed to demonstrate the proof-of-concept with promising applications for DNA/RNA detection and the underlying mechanism for detection of DNA/RNA with the higher sensitivity and selectivity. Furthermore, a brief discussion of common unresolved issues and future trends in this field is provided both from fundamental and practical point of view.
journal_name
Adv Clin Chemjournal_title
Advances in clinical chemistryauthors
Zhou H,Liu J,Xu JJ,Zhang S,Chen HYdoi
10.1016/bs.acc.2019.03.002subject
Has Abstractpub_date
2019-01-01 00:00:00pages
31-98eissn
0065-2423issn
2162-9471pii
S0065-2423(19)30022-8journal_volume
91pub_type
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journal_title:Advances in clinical chemistry
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journal_title:Advances in clinical chemistry
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journal_title:Advances in clinical chemistry
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journal_title:Advances in clinical chemistry
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journal_title:Advances in clinical chemistry
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journal_title:Advances in clinical chemistry
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journal_title:Advances in clinical chemistry
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journal_title:Advances in clinical chemistry
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journal_title:Advances in clinical chemistry
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journal_title:Advances in clinical chemistry
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journal_title:Advances in clinical chemistry
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journal_title:Advances in clinical chemistry
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journal_title:Advances in clinical chemistry
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journal_title:Advances in clinical chemistry
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journal_title:Advances in clinical chemistry
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