Abstract:
BACKGROUND:Recent advances in genome editing have facilitated the direct manipulation of not only the genome, but also the epigenome. Genome editing is typically performed by introducing a single CRISPR/Cas9-mediated double-strand break (DSB), followed by non-homologous end joining (NHEJ)- or homology-directed repair-mediated repair. Epigenome editing, and in particular methylation of CpG dinucleotides, can be performed using catalytically inactive Cas9 (dCas9) fused to a methyltransferase domain. However, for investigations of the role of methylation in gene silencing, studies based on dCas9-methyltransferase have limited resolution and are potentially confounded by the effects of binding of the fusion protein. As an alternative strategy for epigenome editing, we tested CRISPR/Cas9 dual cutting of the genome in the presence of in vitro methylated exogenous DNA, with the aim of driving replacement of the DNA sequence intervening the dual cuts via NHEJ. RESULTS:In a proof of concept at the HPRT1 promoter, successful replacement events with heavily methylated alleles of a CpG island resulted in functional silencing of the HPRT1 gene. Although still limited in efficiency, our study demonstrates concurrent epigenome and genome editing in a single event. CONCLUSIONS:This study opens the door to investigations of the functional consequences of methylation patterns at single CpG dinucleotide resolution. Our results furthermore support the conclusion that promoter methylation is sufficient to functionally silence gene expression.
journal_name
BMC Bioljournal_title
BMC biologyauthors
Alexander J,Findlay GM,Kircher M,Shendure Jdoi
10.1186/s12915-019-0711-zsubject
Has Abstractpub_date
2019-11-18 00:00:00pages
90issue
1issn
1741-7007pii
10.1186/s12915-019-0711-zjournal_volume
17pub_type
杂志文章相关文献
BMC BIOLOGY文献大全abstract:BACKGROUND:Within chromatin, the histone variant H2A.Z plays a role in many diverse nuclear processes including transcription, preventing the spread of heterochromatin and epigenetic transcriptional memory. The molecular mechanisms of how H2A.Z mediates its effects are not entirely understood. However, it is now known ...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-7-86
更新日期:2009-12-14 00:00:00
abstract:BACKGROUND:The Drosophila abnormal wing discs (awd) belongs to a highly conserved family of genes implicated in metastasis suppression, metabolic homeostasis and epithelial morphogenesis. The cellular function of the mammalian members of this family, the Nm23 proteins, has not yet been clearly defined. Previous awd gen...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-12-12
更新日期:2014-02-14 00:00:00
abstract:BACKGROUND:Increasing evidence suggests that influenza reassortment not only contributes to the emergence of new human pandemics but also plays an important role in seasonal influenza epidemics, disease severity, evolution, and vaccine efficacy. We studied this process within 2091 H3N2 full genomes utilizing a combinat...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-016-0337-3
更新日期:2016-12-29 00:00:00
abstract:BACKGROUND:Drosophila melanogaster has served as a powerful model system for genetic studies of courtship songs. To accelerate research on the genetic and neural mechanisms underlying courtship song, we have developed a sensitive recording system to simultaneously capture the acoustic signals from 32 separate pairs of ...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-11-11
更新日期:2013-01-31 00:00:00
abstract:BACKGROUND:mTOR signaling is an essential nutrient and energetic sensing pathway. Here we describe AIMTOR, a sensitive genetically encoded BRET (Bioluminescent Resonance Energy Transfer) biosensor to study mTOR activity in living cells. RESULTS:As a proof of principle, we show in both cell lines and primary cell cultu...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-020-00790-8
更新日期:2020-07-03 00:00:00
abstract:BACKGROUND:A dearth of laboratory tests to study actual human approach-avoidance behavior has complicated translational research on anxiety. The elevated plus-maze (EPM) is the gold standard to assess approach-avoidance behavior in rodents. METHODS:Here, we translated the EPM to humans using mixed reality through a co...
journal_title:BMC biology
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12915-017-0463-6
更新日期:2017-12-21 00:00:00
abstract::Ethylene gas is a major plant hormone that influences diverse processes in plant growth, development and stress responses throughout the plant life cycle. Responses to ethylene, such as fruit ripening, are significant to agriculture. The core molecular elements of the ethylene-signaling pathway have been uncovered, re...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-016-0230-0
更新日期:2016-01-27 00:00:00
abstract:BACKGROUND:In HIV-1-infected human monocyte-derived macrophages (MDMs), virus particles assemble primarily on intracellularly sequestered plasma membrane domains termed intracellular plasma membrane-connected compartments (IPMCs). Despite their clear role in virus formation, little is known of the organization, composi...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-11-89
更新日期:2013-08-02 00:00:00
abstract:BACKGROUND:The ribonuclear protein TDP-43 has been implicated in the pathophysiology of amyotrophic lateral sclerosis (ALS), with genetic mutations being linked to the neurological symptoms of the disease. Though alterations in the intracellular distribution of TDP-43 have been observed in skeletal muscles of patients ...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-020-00767-7
更新日期:2020-03-26 00:00:00
abstract::Protein conformational dynamics simultaneously allow promiscuity and specificity in binding. The multiple conformations of the free EphA4 ligand-binding domain observed in two new EphA4 crystal structures provide a unique insight into the conformational dynamics of EphA4 and its signaling pathways. The heterogeneous e...
journal_title:BMC biology
pub_type: 社论
doi:10.1186/1741-7007-10-2
更新日期:2012-01-25 00:00:00
abstract::In the ubiquitin-proteasome system, a subset of ubiquitylated proteins requires the AAA+ ATPase p97 (also known as VCP or Cdc48) for extraction from membranes or protein complexes before delivery to the proteasome for degradation. Diverse ubiquitin adapters are known to link p97 to its client proteins, but two recent ...
journal_title:BMC biology
pub_type: 社论
doi:10.1186/1741-7007-10-48
更新日期:2012-06-13 00:00:00
abstract::Exosomes are extracellular vesicles first described as such 30 years ago and since implicated in cell-cell communication and the transmission of disease states, and explored as a means of drug discovery. Yet fundamental questions about their biology remain unanswered. Here I explore what exosomes are, highlight the di...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-016-0268-z
更新日期:2016-06-13 00:00:00
abstract::The vertebrate nervous system is deeply divided into 'somatic' and 'visceral' subsystems that respond to external and internal stimuli, respectively. Molecular characterization of neurons in different groups of mollusks by Nomaksteinsky and colleagues, published in this issue of BMC Biology, reveals that the viscero-s...
journal_title:BMC biology
pub_type: 评论,杂志文章
doi:10.1186/1741-7007-11-54
更新日期:2013-04-30 00:00:00
abstract:BACKGROUND:Insects helped pioneer, and persist as model organisms for, the study of specific aspects of immunity. Although they lack an adaptive immune system, insects possess an innate immune system that recognizes and destroys intruding microorganisms. Its operation under natural conditions has not been well studied,...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-5-56
更新日期:2007-12-21 00:00:00
abstract:BACKGROUND:ADP-ribosylation is a ubiquitous post-translational modification that involves both mono- and poly-ADP-ribosylation. ARTD10, also known as PARP10, mediates mono-ADP-ribosylation (MARylation) of substrate proteins. A previous screen identified protein kinase C delta (PKCδ) as a potential ARTD10 substrate, amo...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-020-00878-1
更新日期:2020-10-15 00:00:00
abstract:BACKGROUND:Polycomb-group genes (PcG) encode proteins that maintain homeotic (Hox) gene repression throughout development. Conversely, trithorax-group (trxG) genes encode positive factors required for maintenance of long term Hox gene activation. Both kinds of factors bind chromatin regions called maintenance elements ...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-4-9
更新日期:2006-04-14 00:00:00
abstract:BACKGROUND:One hundred years ago, marine organisms were the dominant systems for the study of developmental biology. The challenges in rearing these organisms outside of a marine setting ultimately contributed to a shift towards work on a smaller number of so-called model systems. Those animals are typically non-marine...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-020-00772-w
更新日期:2020-05-04 00:00:00
abstract:BACKGROUND:Functional characterisation of the compact genome of the model organism Caenorhabditis elegans remains incomplete despite its sequencing 20 years ago. The last decade of research has seen a tremendous increase in the number of non-coding RNAs identified in various organisms. While we have mechanistic underst...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-019-0635-7
更新日期:2019-02-18 00:00:00
abstract:BACKGROUND:The discharge of the Cnidarian stinging organelle, the nematocyst, is one of the fastest processes in biology and involves volume changes of the highly pressurised (150 bar) capsule of up to 50%. Hitherto, the molecular basis for the unusual biomechanical properties of nematocysts has been elusive, as their ...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-014-0113-1
更新日期:2015-01-16 00:00:00
abstract:BACKGROUND:Base editors are a class of genome editing tools with the ability to efficiently induce point mutations in genomic DNA, without inducing double-strand breaks or relying on homology-direct repair as in other such technologies. Recently, adenine base editors (ABEs) have been developed to mediate the conversion...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-018-0609-1
更新日期:2018-11-20 00:00:00
abstract:BACKGROUND:Gq is a heterotrimeric G protein that plays an important role in numerous physiological processes. To delineate the molecular mechanisms and kinetics of signalling through this protein, its activation should be measurable in single living cells. Recently, fluorescence resonance energy transfer (FRET) sensors...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-9-32
更新日期:2011-05-27 00:00:00
abstract:BACKGROUND:Root and tuber crops are a major food source in tropical Africa. Among these crops are several species in the monocotyledonous genus Dioscorea collectively known as yam, a staple tuber crop that contributes enormously to the subsistence and socio-cultural lives of millions of people, principally in West and ...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-017-0419-x
更新日期:2017-09-19 00:00:00
abstract::The five most pervasive anthropogenic threats to biodiversity are over-exploitation, habitat changes, climate change, invasive species, and pollution. Since all of these threats can affect intraspecific biodiversity-including genetic variation within populations-humans have the potential to induce contemporary microev...
journal_title:BMC biology
pub_type: 杂志文章,评审
doi:10.1186/s12915-017-0476-1
更新日期:2018-01-15 00:00:00
abstract::Joel Dacks is an Associate Professor and Canada Research Chair in Evolutionary Cell Biology at the University of Alberta, a Scientific Associate at the Natural History Museum (London), and the current President of the International Society for Evolutionary Protistology. His research group studies the evolution and div...
journal_title:BMC biology
pub_type: 传,历史文章,面试
doi:10.1186/s12915-018-0586-4
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND:Ionotropic receptors (IRs) are a large, divergent subfamily of ionotropic glutamate receptors (iGluRs) that are expressed in diverse peripheral sensory neurons and function in olfaction, taste, hygrosensation and thermosensation. Analogous to the cell biological properties of their synaptic iGluR ancestors, ...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-019-0651-7
更新日期:2019-04-17 00:00:00
abstract:BACKGROUND:Signaling by the Wnt family of secreted glycoproteins through their receptors, the frizzled (Fz) family of seven-pass transmembrane proteins, is critical for numerous cell fate and tissue polarity decisions during development. RESULTS:We report a novel role of Wnt signaling in organogenesis using the format...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-3-23
更新日期:2005-10-24 00:00:00
abstract:BACKGROUND:Pneumococcal meningitis is associated with high mortality (approximately 30%) and morbidity. Up to 50% of survivors are affected by neurological sequelae due to a wide spectrum of brain injury mainly affecting the cortex and hippocampus. Despite this significant disease burden, the genetic program that regul...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-4-15
更新日期:2006-06-02 00:00:00
abstract:BACKGROUND:Sec8 is highly expressed in mammalian nervous systems and has been proposed to play a role in several aspects of neural development and function, including neurite outgrowth, calcium-dependent neurotransmitter secretion, trafficking of ionotropic glutamate receptors and regulation of neuronal microtubule ass...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-3-27
更新日期:2005-12-13 00:00:00
abstract:BACKGROUND:Conditional gene knockout (cKO) mediated by the Cre/LoxP system is indispensable for exploring gene functions in mice. However, a major limitation of this method is that gene KO is not reversible. A number of methods have been developed to overcome this, but each method has its own limitations. RESULTS:We d...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-10-96
更新日期:2012-11-30 00:00:00
abstract:BACKGROUND:Metazoan lineages exhibit a wide range of regenerative capabilities that vary among developmental stage and tissue type. The most robust regenerative abilities are apparent in the phyla Cnidaria, Platyhelminthes, and Echinodermata, whose members are capable of whole-body regeneration (WBR). This phenomenon h...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-019-0633-9
更新日期:2019-02-22 00:00:00