Real-time visualization of heterotrimeric G protein Gq activation in living cells.

abstract：:Few would argue that science is better done in silos, with no transparency or sharing of methods and resources. Yet scientists and scientific stakeholders (e.g., academic institutions, funding agencies, journals) alike continue to find themselves at a relative impasse in the implementation of open science practices, s...

journal_title：BMC biology

authors： Milham MP,Klein A

更新日期：2019-03-27 00:00:00

abstract：BACKGROUND:Ionotropic receptors (IRs) are a large, divergent subfamily of ionotropic glutamate receptors (iGluRs) that are expressed in diverse peripheral sensory neurons and function in olfaction, taste, hygrosensation and thermosensation. Analogous to the cell biological properties of their synaptic iGluR ancestors, ...

journal_title：BMC biology

authors： Abuin L,Prieto-Godino LL,Pan H,Gutierrez C,Huang L,Jin R,Benton R

更新日期：2019-04-17 00:00:00

abstract：BACKGROUND:Metazoan lineages exhibit a wide range of regenerative capabilities that vary among developmental stage and tissue type. The most robust regenerative abilities are apparent in the phyla Cnidaria, Platyhelminthes, and Echinodermata, whose members are capable of whole-body regeneration (WBR). This phenomenon h...

journal_title：BMC biology

authors： Cary GA,Wolff A,Zueva O,Pattinato J,Hinman VF

更新日期：2019-02-22 00:00:00

abstract：BACKGROUND:Base editors are a class of genome editing tools with the ability to efficiently induce point mutations in genomic DNA, without inducing double-strand breaks or relying on homology-direct repair as in other such technologies. Recently, adenine base editors (ABEs) have been developed to mediate the conversion...

journal_title：BMC biology

authors： Qin W,Lu X,Liu Y,Bai H,Li S,Lin S

更新日期：2018-11-20 00:00:00

abstract：BACKGROUND:ADP-ribosylation is a ubiquitous post-translational modification that involves both mono- and poly-ADP-ribosylation. ARTD10, also known as PARP10, mediates mono-ADP-ribosylation (MARylation) of substrate proteins. A previous screen identified protein kinase C delta (PKCδ) as a potential ARTD10 substrate, amo...

journal_title：BMC biology

authors： Tian Y,Korn P,Tripathi P,Komnig D,Wiemuth D,Nikouee A,Classen A,Bolm C,Falkenburger BH,Lüscher B,Gründer S

更新日期：2020-10-15 00:00:00

abstract：:Cells are highly complex and orderly machines, with defined shapes and a startling variety of internal organizations. Complex geometry is a feature of both free-living unicellular organisms and cells inside multicellular animals. Where does the geometry of a cell come from? Many of the same questions that arise in dev...

journal_title：BMC biology

authors： Marshall WF

更新日期：2011-08-31 00:00:00

abstract：BACKGROUND:Seed plants are composed of angiosperms and gymnosperms, which diverged from each other around 300 million years ago. While much light has been shed on the mechanisms and rate of genome evolution in flowering plants, such knowledge remains conspicuously meagre for the gymnosperms. Conifers are key representa...

journal_title：BMC biology

authors： Pavy N,Pelgas B,Laroche J,Rigault P,Isabel N,Bousquet J

更新日期：2012-10-26 00:00:00

abstract：BACKGROUND:A genome-wide association study (GWAS) is the foremost strategy used for finding genes that control human diseases and agriculturally important traits, but it often reports false positives. In contrast, its complementary method, linkage analysis, provides direct genetic confirmation, but with limited resolut...

journal_title：BMC biology

authors： Li C,Li Y,Bradbury PJ,Wu X,Shi Y,Song Y,Zhang D,Rodgers-Melnick E,Buckler ES,Zhang Z,Li Y,Wang T

更新日期：2015-09-21 00:00:00

abstract：BACKGROUND:Sexual reproduction entails the encounter of the sexes and the multiplicity of rituals is parallel to the diversity of mating systems. Evolutionary mechanisms such as sexual selection and sexual conflict have led to the elaboration of traits to gain attention and favours from potential partners. A paradox ex...

journal_title：BMC biology

authors： Lassance JM,Löfstedt C

更新日期：2009-03-03 00:00:00

abstract：BACKGROUND:Astrocytes can mediate neurovascular coupling, modulate neuronal excitability, and promote synaptic maturation and remodeling. All these functions are likely to be modulated by the sleep/wake cycle, because brain metabolism, neuronal activity and synaptic turnover change as a function of behavioral state. Ye...

journal_title：BMC biology

authors： Bellesi M,de Vivo L,Tononi G,Cirelli C

更新日期：2015-08-25 00:00:00

abstract：:The majority of convincingly documented cases of hybridization in angiosperms has involved genetic introgression between the parental species or formation of a hybrid species with increased ploidy; however, homoploid (diploid) hybridization may be just as common. Recent studies, including one in BMC Evolutionary Biolo...

journal_title：BMC biology

authors： Chase MW,Paun O,Fay MF

更新日期：2010-04-21 00:00:00

abstract：BACKGROUND:Internal tagging of proteins by inserting small functional peptides into surface accessible permissive sites has proven to be an indispensable tool for basic and applied science. Permissive sites are typically identified by transposon mutagenesis on a case-by-case basis, limiting scalability and their exploi...

journal_title：BMC biology

authors： Oesterle S,Roberts TM,Widmer LA,Mustafa H,Panke S,Billerbeck S

更新日期：2017-10-30 00:00:00

abstract：BACKGROUND:mTOR signaling is an essential nutrient and energetic sensing pathway. Here we describe AIMTOR, a sensitive genetically encoded BRET (Bioluminescent Resonance Energy Transfer) biosensor to study mTOR activity in living cells. RESULTS:As a proof of principle, we show in both cell lines and primary cell cultu...

journal_title：BMC biology

authors： Bouquier N,Moutin E,Tintignac LA,Reverbel A,Jublanc E,Sinnreich M,Chastagnier Y,Averous J,Fafournoux P,Verpelli C,Boeckers T,Carnac G,Perroy J,Ollendorff V

更新日期：2020-07-03 00:00:00

abstract：BACKGROUND:A new family of natural products has been described in which cysteine, serine and threonine from ribosomally-produced peptides are converted to thiazoles, oxazoles and methyloxazoles, respectively. These metabolites and their biosynthetic gene clusters are now referred to as thiazole/oxazole-modified microci...

journal_title：BMC biology

authors： Haft DH,Basu MK,Mitchell DA

更新日期：2010-05-25 00:00:00

abstract：BACKGROUND:The most commonly occurring cancer mutations, including oncogenes such as MYC, Ras and PIK3C, are found in signal transductions pathways feeding into the translational machinery. A broad range of translation initiation factors are also commonly found to be either amplified or mis-regulated in tumours, includ...

journal_title：BMC biology

authors： Frosi Y,Usher R,Lian DTG,Lane DP,Brown CJ

更新日期：2019-05-22 00:00:00

abstract：BACKGROUND:Cellular functions can be regulated by cell-cell interactions that are influenced by extra-cellular, density-dependent signaling factors. Dictyostelium grow as individual cells in nutrient-rich sources, but, as nutrients become depleted, they initiate a multi-cell developmental program that is dependent upon...

journal_title：BMC biology

authors： Meena NP,Jaiswal P,Chang FS,Brzostowski J,Kimmel AR

更新日期：2019-12-02 00:00:00

abstract：BACKGROUND:Membrane proteins form key nodes in mediating the cell's interaction with the surroundings, which is one of the main reasons why the majority of drug targets are membrane proteins. RESULTS:Here we mined the human proteome and identified the membrane proteome subset using three prediction tools for alpha-hel...

journal_title：BMC biology

authors： Almén MS,Nordström KJ,Fredriksson R,Schiöth HB

更新日期：2009-08-13 00:00:00

abstract：BACKGROUND:Complex multicellularity requires elaborate developmental mechanisms, often based on the versatility of heterodimeric transcription factor (TF) interactions. Homeobox TFs in the TALE superclass are deeply embedded in the gene regulatory networks that orchestrate embryogenesis. Knotted-like homeobox (KNOX) TF...

journal_title：BMC biology

authors： Joo S,Wang MH,Lui G,Lee J,Barnas A,Kim E,Sudek S,Worden AZ,Lee JH

更新日期：2018-11-05 00:00:00

abstract：BACKGROUND:The traditional concept that heritability occurs exclusively from the transfer of germline-restricted genetics is being challenged by the increasing accumulation of evidence confirming the existence of experience-dependent transgenerational inheritance. However, questions remain unanswered as to how heritabl...

journal_title：BMC biology

authors： O'Brien EA,Ensbey KS,Day BW,Baldock PA,Barry G

更新日期：2020-04-30 00:00:00

abstract：:The close association between hematopoietic and endothelial cells during embryonic development led to the proposal that they may originate from a common ancestor--the hemangioblast. Due to a lack of unique specific markers for in vivo cell fate tracking studies, evidence supporting this theory derives mainly from in v...

journal_title：BMC biology

authors： Gordon-Keylock S,Medvinsky A

更新日期：2011-12-28 00:00:00

abstract：BACKGROUND:Kinases mTORC1 and AMPK act as energy sensors, controlling nutrient responses and cellular growth. Changes in nutrient levels affect diverse transcriptional networks, making it challenging to identify downstream paths that regulate cellular growth or a switch to development via nutrient variation. The life c...

journal_title：BMC biology

authors： Jaiswal P,Kimmel AR

更新日期：2019-07-18 00:00:00

abstract：BACKGROUND:The Drosophila abnormal wing discs (awd) belongs to a highly conserved family of genes implicated in metastasis suppression, metabolic homeostasis and epithelial morphogenesis. The cellular function of the mammalian members of this family, the Nm23 proteins, has not yet been clearly defined. Previous awd gen...

journal_title：BMC biology

authors： Ignesti M,Barraco M,Nallamothu G,Woolworth JA,Duchi S,Gargiulo G,Cavaliere V,Hsu T

更新日期：2014-02-14 00:00:00

abstract：BACKGROUND:Many Caenorhabditis elegans mutations increase longevity and much evidence suggests that they do so at least partly via changes in metabolism. However, up until now there has been no systematic investigation of how the metabolic networks of long-lived mutants differ from those of normal worms. Metabolomic te...

journal_title：BMC biology

authors： Fuchs S,Bundy JG,Davies SK,Viney JM,Swire JS,Leroi AM

更新日期：2010-02-10 00:00:00

abstract：BACKGROUND:The western flower thrips, Frankliniella occidentalis (Pergande), is a globally invasive pest and plant virus vector on a wide array of food, fiber, and ornamental crops. The underlying genetic mechanisms of the processes governing thrips pest and vector biology, feeding behaviors, ecology, and insecticide r...

journal_title：BMC biology

authors： Rotenberg D,Baumann AA,Ben-Mahmoud S,Christiaens O,Dermauw W,Ioannidis P,Jacobs CGC,Vargas Jentzsch IM,Oliver JE,Poelchau MF,Rajarapu SP,Schneweis DJ,Snoeck S,Taning CNT,Wei D,Widana Gamage SMK,Hughes DST,Murali SC,Ba

更新日期：2020-10-19 00:00:00

abstract：BACKGROUND:The study of microbial communities has been revolutionised in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. One potential confounder of these sequence-based approaches is the presence of contamination in DNA extraction ...

journal_title：BMC biology

authors： Salter SJ,Cox MJ,Turek EM,Calus ST,Cookson WO,Moffatt MF,Turner P,Parkhill J,Loman NJ,Walker AW

更新日期：2014-11-12 00:00:00

abstract：BACKGROUND:Invariant natural killer T (iNKT) cells differ from other T cells by their hyperactive effector T-cell status, in addition to the expression of NK lineage receptors and semi-invariant T-cell receptors. It is generally agreed that the immune phenotype of iNKT cells is maintained by repeated activation in peri...

journal_title：BMC biology

authors： Zeng J,Howard JC

更新日期：2010-11-30 00:00:00

abstract：BACKGROUND:The red algae (Rhodophyta) diverged from the green algae and plants (Viridiplantae) over one billion years ago within the kingdom Archaeplastida. These photosynthetic lineages provide an ideal model to study plastid genome reduction in deep time. To this end, we assembled a large dataset of the plastid genom...

journal_title：BMC biology

authors： Lee J,Cho CH,Park SI,Choi JW,Song HS,West JA,Bhattacharya D,Yoon HS

更新日期：2016-09-02 00:00:00

abstract：:The global spread of SARS-CoV-2 has shaken our health care and economic systems, prompting re-evaluation of long-held views on how best to deliver care. This is especially the case for our global diagnostic strategy. While current laboratory-based centralized RT-qPCR will continue to serve as a gold standard diagnosti...

journal_title：BMC biology

authors： Matthews Q,da Silva SJR,Norouzi M,Pena LJ,Pardee K

更新日期：2020-10-28 00:00:00

abstract：BACKGROUND:Annotation of cell identity is an essential process in neuroscience that allows comparison of cells, including that of neural activities across different animals. In Caenorhabditis elegans, although unique identities have been assigned to all neurons, the number of annotatable neurons in an intact animal has...

journal_title：BMC biology

authors： Toyoshima Y,Wu S,Kanamori M,Sato H,Jang MS,Oe S,Murakami Y,Teramoto T,Park C,Iwasaki Y,Ishihara T,Yoshida R,Iino Y

更新日期：2020-03-19 00:00:00

abstract：BACKGROUND:Management of blood cholesterol is a major focus of efforts to prevent cardiovascular diseases. The objective of this study was to investigate how the gut microbiota affects host cholesterol homeostasis at the organism scale. RESULTS:We depleted the intestinal microbiota of hypercholesterolemic female Apoe-...

journal_title：BMC biology

authors： Le Roy T,Lécuyer E,Chassaing B,Rhimi M,Lhomme M,Boudebbouze S,Ichou F,Haro Barceló J,Huby T,Guerin M,Giral P,Maguin E,Kapel N,Gérard P,Clément K,Lesnik P

更新日期：2019-11-27 00:00:00