Abstract：
BACKGROUND:Astrocytes can mediate neurovascular coupling, modulate neuronal excitability, and promote synaptic maturation and remodeling. All these functions are likely to be modulated by the sleep/wake cycle, because brain metabolism, neuronal activity and synaptic turnover change as a function of behavioral state. Yet, little is known about the effects of sleep and wake on astrocytes. RESULTS:Here we show that sleep and wake strongly affect both astrocytic gene expression and ultrastructure in the mouse brain. Using translating ribosome affinity purification technology and microarrays, we find that 1.4 % of all astrocytic transcripts in the forebrain are dependent on state (three groups, sleep, wake, short sleep deprivation; six mice per group). Sleep upregulates a few select genes, like Cirp and Uba1, whereas wake upregulates many genes related to metabolism, the extracellular matrix and cytoskeleton, including Trio, Synj2 and Gem, which are involved in the elongation of peripheral astrocytic processes. Using serial block face scanning electron microscopy (three groups, sleep, short sleep deprivation, chronic sleep restriction; three mice per group, >100 spines per mouse, 3D), we find that a few hours of wake are sufficient to bring astrocytic processes closer to the synaptic cleft, while chronic sleep restriction also extends the overall astrocytic coverage of the synapse, including at the axon-spine interface, and increases the available astrocytic surface in the neuropil. CONCLUSIONS:Wake-related changes likely reflect an increased need for glutamate clearance, and are consistent with an overall increase in synaptic strength when sleep is prevented. The reduced astrocytic coverage during sleep, instead, may favor glutamate spillover, thus promoting neuronal synchronization during non-rapid eye movement sleep.
journal_name
BMC Bioljournal_title
BMC biologyauthors
Bellesi M,de Vivo L,Tononi G,Cirelli Cdoi
10.1186/s12915-015-0176-7subject
Has Abstractpub_date
2015-08-25 00:00:00pages
66issn
1741-7007pii
10.1186/s12915-015-0176-7journal_volume
13pub_type
杂志文章相关文献
BMC BIOLOGY文献大全abstract：BACKGROUND:Kinases mTORC1 and AMPK act as energy sensors, controlling nutrient responses and cellular growth. Changes in nutrient levels affect diverse transcriptional networks, making it challenging to identify downstream paths that regulate cellular growth or a switch to development via nutrient variation. The life c...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/s12915-019-0673-1
更新日期：2019-07-18 00:00:00
abstract：BACKGROUND:Metazoan lineages exhibit a wide range of regenerative capabilities that vary among developmental stage and tissue type. The most robust regenerative abilities are apparent in the phyla Cnidaria, Platyhelminthes, and Echinodermata, whose members are capable of whole-body regeneration (WBR). This phenomenon h...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/s12915-019-0633-9
更新日期：2019-02-22 00:00:00
abstract：BACKGROUND:Functional characterisation of the compact genome of the model organism Caenorhabditis elegans remains incomplete despite its sequencing 20 years ago. The last decade of research has seen a tremendous increase in the number of non-coding RNAs identified in various organisms. While we have mechanistic underst...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/s12915-019-0635-7
更新日期：2019-02-18 00:00:00
abstract：BACKGROUND:Recent advances in genome editing have facilitated the direct manipulation of not only the genome, but also the epigenome. Genome editing is typically performed by introducing a single CRISPR/Cas9-mediated double-strand break (DSB), followed by non-homologous end joining (NHEJ)- or homology-directed repair-m...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/s12915-019-0711-z
更新日期：2019-11-18 00:00:00
abstract：BACKGROUND:The green algae Chlamydomonas reinhardtii and Volvox carteri are important models for studying light perception and response, expressing many different photoreceptors. More than 10 opsins were reported in C. reinhardtii, yet only two-the channelrhodopsins-were functionally characterized. Characterization of ...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/s12915-018-0613-5
更新日期：2018-12-06 00:00:00
abstract：BACKGROUND:Cell migration is essential for development and tissue repair, but it also contributes to disease. Rho GTPases regulate cell migration, but a comprehensive analysis of how each Rho signalling component affects migration has not been carried out. RESULTS:Through an RNA interference screen, and using a prosta...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/s12915-018-0489-4
更新日期：2018-03-06 00:00:00
abstract：BACKGROUND:Invariant natural killer T (iNKT) cells differ from other T cells by their hyperactive effector T-cell status, in addition to the expression of NK lineage receptors and semi-invariant T-cell receptors. It is generally agreed that the immune phenotype of iNKT cells is maintained by repeated activation in peri...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/1741-7007-8-142
更新日期：2010-11-30 00:00:00
abstract：BACKGROUND:Marine molluscs, as is the case with most aquatic animals, rely heavily on olfactory cues for survival. In the mollusc Aplysia californica, mate-attraction is mediated by a blend of water-borne protein pheromones that are detected by sensory structures called rhinophores. The expression of G protein and phos...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/1741-7007-7-28
更新日期：2009-06-04 00:00:00
abstract：BACKGROUND:Seed plants are composed of angiosperms and gymnosperms, which diverged from each other around 300 million years ago. While much light has been shed on the mechanisms and rate of genome evolution in flowering plants, such knowledge remains conspicuously meagre for the gymnosperms. Conifers are key representa...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/1741-7007-10-84
更新日期：2012-10-26 00:00:00
abstract：BACKGROUND:The hormone adiponectin (ApN) exerts powerful anti-inflammatory effects on skeletal muscle and can reverse devastating myopathies, like Duchenne muscular dystrophy (DMD), where inflammation exacerbates disease progression. The NLRP3 inflammasome plays a key role in the inflammation process, and its aberrant ...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/s12915-018-0501-z
更新日期：2018-03-20 00:00:00
abstract：BACKGROUND:Genetically encoded calcium ion (Ca2+) indicators (GECIs) are indispensable tools for measuring Ca2+ dynamics and neuronal activities in vitro and in vivo. Red fluorescent protein (RFP)-based GECIs have inherent advantages relative to green fluorescent protein-based GECIs due to the longer wavelength light u...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/s12915-018-0480-0
更新日期：2018-01-16 00:00:00
abstract：BACKGROUND:Dorsal spine reduction in threespine sticklebacks (Gasterosteus aculeatus) is a classic example of recurrent skeletal evolution in nature. Sticklebacks in marine environments typically have long spines that form part of their skeletal armor. Many derived freshwater populations have evolved shorter spines. Ch...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/s12915-017-0456-5
更新日期：2017-12-07 00:00:00
abstract：BACKGROUND:Effective disease management depends on timely and accurate diagnosis to guide control measures. The capacity to distinguish between individuals in a pathogen population with specific properties such as fungicide resistance, toxin production and virulence profiles is often essential to inform disease managem...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/s12915-019-0684-y
更新日期：2019-08-13 00:00:00
abstract：BACKGROUND:Parkinson's disease (PD)-associated E3 ubiquitin ligase Parkin is enriched at glutamatergic synapses, where it ubiquitinates multiple substrates, suggesting that its mutation/loss-of-function could contribute to the etiology of PD by disrupting excitatory neurotransmission. Here, we evaluate the impact of fo...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/s12915-018-0567-7
更新日期：2018-09-10 00:00:00
abstract：BACKGROUND:One hundred years ago, marine organisms were the dominant systems for the study of developmental biology. The challenges in rearing these organisms outside of a marine setting ultimately contributed to a shift towards work on a smaller number of so-called model systems. Those animals are typically non-marine...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/s12915-020-00772-w
更新日期：2020-05-04 00:00:00
abstract：BACKGROUND:The transmissible spongiform encephalopathies, otherwise known as prion diseases, occur following the conversion of the cellular prion protein (PrPC) to an alternatively folded, disease-associated isoform (PrPSc). Recent studies suggest that this conversion occurs via a cholesterol-sensitive process, as chol...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/1741-7007-6-39
更新日期：2008-09-12 00:00:00
abstract：BACKGROUND:Signaling by the Wnt family of secreted glycoproteins through their receptors, the frizzled (Fz) family of seven-pass transmembrane proteins, is critical for numerous cell fate and tissue polarity decisions during development. RESULTS:We report a novel role of Wnt signaling in organogenesis using the format...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/1741-7007-3-23
更新日期：2005-10-24 00:00:00
abstract：BACKGROUND:Many Caenorhabditis elegans mutations increase longevity and much evidence suggests that they do so at least partly via changes in metabolism. However, up until now there has been no systematic investigation of how the metabolic networks of long-lived mutants differ from those of normal worms. Metabolomic te...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/1741-7007-8-14
更新日期：2010-02-10 00:00:00
abstract：:The expansion of genome sequencing projects has produced accumulating evidence for lateral transfer of genes between prokaryotic and eukaryotic genomes. However, it remains controversial whether these genes are of functional importance in their recipient host. Nikoh and Nakabachi, in a recent paper in BMC Biology, tak...
journal_title：BMC biology
pub_type： 评论,杂志文章
doi：10.1186/1741-7007-7-20
更新日期：2009-05-05 00:00:00
abstract：BACKGROUND:Dinoflagellates are taxonomically diverse and ecologically important phytoplankton that are ubiquitously present in marine and freshwater environments. Mostly photosynthetic, dinoflagellates provide the basis of aquatic primary production; most taxa are free-living, while some can form symbiotic and parasiti...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/s12915-020-00782-8
更新日期：2020-05-24 00:00:00
abstract：BACKGROUND:A dearth of laboratory tests to study actual human approach-avoidance behavior has complicated translational research on anxiety. The elevated plus-maze (EPM) is the gold standard to assess approach-avoidance behavior in rodents. METHODS:Here, we translated the EPM to humans using mixed reality through a co...
journal_title：BMC biology
pub_type： 杂志文章,随机对照试验
doi：10.1186/s12915-017-0463-6
更新日期：2017-12-21 00:00:00
abstract：BACKGROUND:Methods for assigning strains to bacterial species are cumbersome and no longer fit for purpose. The concatenated sequences of multiple house-keeping genes have been shown to be able to define and circumscribe bacterial species as sequence clusters. The advantage of this approach (multilocus sequence analysi...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/1741-7007-7-3
更新日期：2009-01-26 00:00:00
abstract：BACKGROUND:Humans die at an increasing rate until late in life, when mortality rates level off. The causes of the late-life mortality plateau have been debated extensively over the past few years. Here, I examine mortality patterns separately for each of the leading causes of death. The different causes of death show d...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/1741-7007-2-16
更新日期：2004-07-08 00:00:00
abstract：BACKGROUND:CRISPR gene drive systems allow the rapid spread of a genetic construct throughout a population. Such systems promise novel strategies for the management of vector-borne diseases and invasive species by suppressing a target population or modifying it with a desired trait. However, current homing-type drives ...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/s12915-020-0761-2
更新日期：2020-03-12 00:00:00
abstract：:The five most pervasive anthropogenic threats to biodiversity are over-exploitation, habitat changes, climate change, invasive species, and pollution. Since all of these threats can affect intraspecific biodiversity-including genetic variation within populations-humans have the potential to induce contemporary microev...
journal_title：BMC biology
pub_type： 杂志文章,评审
doi：10.1186/s12915-017-0476-1
更新日期：2018-01-15 00:00:00
abstract：BACKGROUND:Mitochondrial DNA (mtDNA) haplotypes have become popular tools for tracing maternal ancestry, and several companies offer this service to the general public. Numerous studies have demonstrated that human mtDNA haplotypes can be used with confidence to identify the continent where the haplotype originated. Id...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/1741-7007-4-34
更新日期：2006-10-12 00:00:00
abstract：:A common symbiont of insects, the bacterium Wolbachia has been implicated in phenomena as diverse as sex determination, pathogen defence and speciation and is being used in public health programs to prevent mosquitoes transmitting disease. Despite decades of research, we know remarkably little about how it exerts its ...
journal_title：BMC biology
pub_type： 社论
doi：10.1186/s12915-016-0312-z
更新日期：2016-10-19 00:00:00
abstract：BACKGROUND:Base editors are a class of genome editing tools with the ability to efficiently induce point mutations in genomic DNA, without inducing double-strand breaks or relying on homology-direct repair as in other such technologies. Recently, adenine base editors (ABEs) have been developed to mediate the conversion...
journal_title：BMC biology
pub_type： 杂志文章
doi：10.1186/s12915-018-0609-1
更新日期：2018-11-20 00:00:00
abstract：:Protein conformational dynamics simultaneously allow promiscuity and specificity in binding. The multiple conformations of the free EphA4 ligand-binding domain observed in two new EphA4 crystal structures provide a unique insight into the conformational dynamics of EphA4 and its signaling pathways. The heterogeneous e...
journal_title：BMC biology
pub_type： 社论
doi：10.1186/1741-7007-10-2
更新日期：2012-01-25 00:00:00
abstract：:The close association between hematopoietic and endothelial cells during embryonic development led to the proposal that they may originate from a common ancestor--the hemangioblast. Due to a lack of unique specific markers for in vivo cell fate tracking studies, evidence supporting this theory derives mainly from in v...
journal_title：BMC biology
pub_type： 社论
doi：10.1186/1741-7007-9-88
更新日期：2011-12-28 00:00:00
