Abstract:
:Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) isolated from the peripheral blood of a five months-old boy with glycogen storage disease type II(GSD II, also known as Pompe disease, PD) carries compound mutations R608X E888X in GAA gene. PBMCs were reprogrammed using non-integrative Sendai viral vectors containing reprogramming factors OCT4, SOX2, KLF4 and C-MYC. iPSCs were shown to express pluripotent markers, have trilineage differentiation potential, carry GAA-R608X and GAA-E888X compound mutations, have a normal karyotype. It is useful tool for studying GSDII.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Zhang Y,Li A,Wang J,Wang G,Wang Ddoi
10.1016/j.scr.2019.101621subject
Has Abstractpub_date
2019-12-01 00:00:00pages
101621eissn
1873-5061issn
1876-7753pii
S1873-5061(19)30251-Xjournal_volume
41pub_type
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journal_title:Stem cell research
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