Abstract:
:Measurement of Alkaline Phosphatase (ALP) level is a widely used procedure in clinical and basic research. We present a simple and inexpensive luminescence-based method that allows multiplexed measurement and normalization of intracellular ALP levels in one sample well. The method comprises two commercially available reagents enabling quantification of ALP levels and cell number by two sequential luminescence readouts. Using this method we were able to detect and analyze somatic reprogramming into pluripotent stem cells. The method is highly applicable for High Throughput Screening (HTS) campaigns and analysis.
journal_name
Stem Cell Resjournal_title
Stem cell researchauthors
Plotnikov A,Kozer N,Krupalnik V,Peles S,Mor N,Rais Y,Hanna JH,Barr HMdoi
10.1016/j.scr.2017.07.014subject
Has Abstractpub_date
2017-08-01 00:00:00pages
158-162eissn
1873-5061issn
1876-7753pii
S1873-5061(17)30138-1journal_volume
23pub_type
杂志文章abstract::Two distinct types of embryonic pluripotent stem cells can be established from either the inner cell mass (ICM) of preimplantation blastocyst (leukemia inhibitory factor (LIF)-dependent embryonic stem cell, ESC, called naive state) or the epiblast of postimplantation fetuses (fibroblast growth factor 2 (FGF2)-dependen...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2014.04.012
更新日期:2014-07-01 00:00:00
abstract::Cartilage defects have limited capacity for repair and are often replaced by fibrocartilage with inferior mechanical properties. To overcome the limitations of artificial joint replacement, high-throughput screens (HTS) could be developed to identify molecules that stimulate differentiation and/or proliferation of art...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2010.08.007
更新日期:2011-01-01 00:00:00
abstract::Ectopic expression of HoxB4 in embryonic stem (ES) cells leads to an efficient production of hematopoietic cells, including hematopoietic stem/progenitor cells. Previous studies have utilized a constitutive HoxB4 expression system or tetracycline-regulated HoxB4 expression system to induce hematopoietic cells from ES ...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2011.09.001
更新日期:2012-03-01 00:00:00
abstract::Maturity onset diabetes of the young (MODY) is a hereditary form of diabetes mellitus presenting at childhood or adolescence, which eventually leads to pancreatic β-cells dysfunction. The underlying genetic basis of MODY disorders is haploinsufficiency, where loss-of-function mutations in a single allele cause the dia...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.06.013
更新日期:2018-08-01 00:00:00
abstract::Niemann-Pick disease type A (NPA) is a rare autosomal recessive lysosomal storage disease caused by mutations in the SMPD1 gene, which encodes for the protein acid sphingomyelinase. A human induced pluripotent stem cell (iPSC) line was generated from dermal fibroblasts of a 21-fetal-week-old female patient with NPA th...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101461
更新日期:2019-07-01 00:00:00
abstract::Myeloid ecotropic viral integration site 1 (MEIS1) plays an essential role in the development of several embryonic organs, such as the central nervous system and eyes. To further investigate the role of MEIS1 in embryonic development, herein, we generated a MEIS1 homozygous knockout human embryonic stem cell (hESC) li...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.102069
更新日期:2020-12-01 00:00:00
abstract::Oligodendrocytes, which are the main cell type in cerebral white matter, are generated from their precursor cells (oligodendrocyte precursor cells: OPCs). However, the differentiation from OPCs to oligodendrocytes is disturbed under stressed conditions. Therefore, drugs that can improve oligodendrocyte regeneration ma...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2015.05.001
更新日期:2015-07-01 00:00:00
abstract::CHCHD2 mutation has been reported as a potential cause of a rare form of familial Parkinson's disease. Recently, a novel CHCHD2 mutation was identified in a family with Parkinson's disease. The dermal fibroblasts of the patient were obtained and successfully transformed into induced pluripotent stem cells(iPSCs), empl...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.08.011
更新日期:2018-10-01 00:00:00
abstract::Conditioned medium (CM) derived from engineered cells often facilitates the cost-effective culture of a variety of stem cells. Growing emphasis on the importance of rigor and reproducibility in lab-based science requires development of best practices approaches, including quality control procedures for the assessment ...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101430
更新日期:2019-05-01 00:00:00
abstract::The outcomes of clinical trials using bone marrow stromal cell (BMSC) are variable; the degree of the expansion of BMSCs during clinical manufacturing may contribute to this variability since cell expansion is limited by senescence. Human BMSCs from aspirates of healthy subjects were subcultured serially until cell gr...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2013.07.005
更新日期:2013-11-01 00:00:00
abstract::Characterisation of mouse pluripotent stem cells has revealed two distinct pluripotent states, naive and primed, that maintain characteristics of the pre and post implanted epiblast respectively. Recent studies have developed several culture systems that seek to recapitulate the naive phenomenon in human pluripotent s...
journal_title:Stem cell research
pub_type: 杂志文章,评审
doi:10.1016/j.scr.2017.11.017
更新日期:2018-01-01 00:00:00
abstract::We generated an induced human pluripotent stem cell line from a child with microcephaly carrying compound heterozygous mutations of TYW1 inherited from healthy parents. This iPS cell line showed typical embryonic stem cell-like morphology, expressed pluripotent markers that comparable to human embryonic stem cells. Mo...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.101783
更新日期:2020-05-01 00:00:00
abstract::Expanded human T cells from a Japanese female with recessive dystrophic epidermolysis bullosa (RDBE) were used to generate integration-free induced pluripotent stem cells (iPSCs) by exogenous expression of four reprogramming factors, OCT3/4, SOX2, cMYC, KLF4, using Sendai virus vector (SeVdp). The authenticity of esta...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2016.05.003
更新日期:2016-07-01 00:00:00
abstract::Little is known about the functions of downstream regulatory element antagonist modulator (DREAM) in embryonic stem cells (ESCs). However, DREAM interacts with cAMP response element-binding protein (CREB) in a Ca(2+)-dependent manner, preventing CREB binding protein (CBP) recruitment. Furthermore, CREB and CBP are inv...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2016.03.001
更新日期:2016-05-01 00:00:00
abstract::Optimization of pluripotent stem cell expansion and differentiation is facilitated by biological tools that permit non-invasive and dynamic monitoring of pluripotency, and the ability to select for an undifferentiated input cell population. Here we report on the generation and characterisation of clonal human embryoni...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2014.05.006
更新日期:2014-09-01 00:00:00
abstract::Neural stem cells (NSCs) have been considered as potential therapy in Alzheimer's disease (AD) but their use is hampered by the poor survival of grafted cells. Supply of neurotrophic factors to the grafted cells has been proposed as a way to augment survival of the stem cells. In this context, we investigated the util...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2015.04.008
更新日期:2015-07-01 00:00:00
abstract::The Mesenchymal-to-Epithelial Transition (MET) has been recognized as a crucial step for successful reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs). Thus, it has been demonstrated, that the efficiency of reprogramming can be enhanced by promoting an epithelial expression program in cells, with a...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2014.11.003
更新日期:2015-01-01 00:00:00
abstract::Maintenance of hematopoietic stem cells and their potential to give rise to progenitors of differentiated lymphoid and myeloid cells are accomplished by a network of regulatory processes. As a part of this network, the heteromeric transcription factor GA-binding protein (GABP) plays a crucial role in self-renewal of m...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2016.04.007
更新日期:2016-05-01 00:00:00
abstract::By means of retroviral transduction using the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC primary human amniotic fluid cells (AFCs) were reprogrammed into several iPSC lines. Pluripotency was confirmed both in vitro and in vivo. A comparative transcriptome analysis of the AF-derived iPSC line 41 and the human emb...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2015.11.003
更新日期:2015-11-01 00:00:00
abstract::Derivation of induced pluripotent stem (iPS) cells is mainly an epigenetic reprogramming process. It is still quite controversial how genomic imprinting is reprogrammed in iPS cells. Thus, we derived multiple iPS clones from genetically identical mouse somatic cells. We found that parentally inherited imprint was vari...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2013.05.011
更新日期:2013-09-01 00:00:00
abstract::Asparagine synthetase (ASNS) deficiency (ASNSD; MIM #615574) is a rare neurodevelopmental disorder caused by mutations in the ASNS gene. The ASNS gene maps to cytogenetic band 7q21.3 and is 35 kb long. ASNSD is characterised by congenital microcephaly, severely delayed psychomotor development, seizures, and hyperekple...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101583
更新日期:2019-12-01 00:00:00
abstract::The aim of this study was to determine whether the growth and differentiation of limbal epithelial stem cell cultures could be controlled through manipulation of the oxygen tension. Limbal epithelial cells were isolated from corneoscleral disks, and cultured using either feeder cells in a growth medium supplemented wi...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2013.01.004
更新日期:2013-05-01 00:00:00
abstract::FOS is component of the AP-1 complex and has been reported to be involved in many cellular functions, including cell proliferation, differentiation, survival, angiogenesis, hematopoiesis and cancer progress. To further understand the exact role of FOS in these processes, here we created two FOS knockout human embryoni...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101479
更新日期:2019-08-01 00:00:00
abstract::ATOX1 is a copper chaperone involved in intracellular copper homeostasis, cell proliferation, and tumor progression. To investigate the physiologically relevant molecular mechanism of ATOX1 by using imaging-based approaches, we genetically modified ATOX1 in H1 hESCs to express mCherry-ATOX1 fusion protein under endoge...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101631
更新日期:2019-12-01 00:00:00
abstract::Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) isolated from the peripheral blood of a five months-old boy with glycogen storage disease type II(GSD II, also known as Pompe disease, PD) carries compound mutations R608X E888X in GAA gene. PBMCs were reprogrammed us...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2019.101621
更新日期:2019-12-01 00:00:00
abstract::Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal multipotent stromal cells carrying two mutations in plakophilin-2 (PKP2) gene using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2, CMYC were delivered using Sendai viruses. Pluripotency was confirmed i...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2017.08.014
更新日期:2017-10-01 00:00:00
abstract::Human pluripotent cells such as human embryonic stem cells (hESC) are a great potential source of cells for cell-based therapies; however, directing their differentiation into the desired cell types with high purity remains a challenge. The stem cell microenvironment plays a vital role in directing hESC fate and we ha...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2008.11.004
更新日期:2009-03-01 00:00:00
abstract::As one of the most essential genome guardians, p53 and its mutants have been suggested associated with many types of cancers. Many p53 mutants function induce unique phenotypes, including carcinogenesis, metastasis, and drug resistance. The p53(R249S) mutation is the most prevalent and specific mutation associated wit...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2018.101360
更新日期:2019-01-01 00:00:00
abstract::Dominant deafness-onychodystrophy (DDOD) syndrome is a rare, autosomal dominant inherited disorder with no concrete therapies in human. We previously identified c.1516 C > T (p.Arg506*) in ATP6V1B2 as cause of DDOD syndrome, accounting for all cases of this genetic disorder. The induced pluripotent stem cell (iPSC) li...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2020.101986
更新日期:2020-10-01 00:00:00
abstract::In the present study, we investigated the effect of simultaneous downregulation of uPAR and cathepsin B (pUC), alone or in combination with radiation, on JNK-MAPK signaling pathway in regulating the migration of non-GICs (glioma-initiating cells) and GICs. The increase in the expression of p-JNK with pUC treatment was...
journal_title:Stem cell research
pub_type: 杂志文章
doi:10.1016/j.scr.2014.02.008
更新日期:2014-05-01 00:00:00