A multiplexed screening method for pluripotency.

Abstract:

:Measurement of Alkaline Phosphatase (ALP) level is a widely used procedure in clinical and basic research. We present a simple and inexpensive luminescence-based method that allows multiplexed measurement and normalization of intracellular ALP levels in one sample well. The method comprises two commercially available reagents enabling quantification of ALP levels and cell number by two sequential luminescence readouts. Using this method we were able to detect and analyze somatic reprogramming into pluripotent stem cells. The method is highly applicable for High Throughput Screening (HTS) campaigns and analysis.

journal_name

Stem Cell Res

journal_title

Stem cell research

authors

Plotnikov A,Kozer N,Krupalnik V,Peles S,Mor N,Rais Y,Hanna JH,Barr HM

doi

10.1016/j.scr.2017.07.014

subject

Has Abstract

pub_date

2017-08-01 00:00:00

pages

158-162

eissn

1873-5061

issn

1876-7753

pii

S1873-5061(17)30138-1

journal_volume

23

pub_type

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