Abstract：
:With its single cell sensitivity over volumes as large as or larger than a mouse, cryo-imaging enables imaging of stem cell biodistribution, homing, engraftment, and molecular mechanisms. We developed and evaluated a highly automated software tool to detect fluorescently labeled stem cells within very large ( ∼ 200 GB) cryo-imaging datasets. Cell detection steps are: preprocess, remove immaterial regions, spatially filter to create features, identify candidate pixels, classify pixels using bagging decision trees, segment cell patches, and perform 3D labeling. There are options for analysis and visualization. To train the classifier, we created synthetic images by placing realistic digital cell models onto cryo-images of control mice devoid of cells. Very good cell detection results were (precision=98.49%, recall=99.97%) for synthetic cryo-images, (precision=97.81%, recall=97.71%) for manually evaluated, actual cryo-images, and false positives in control mice. An α-multiplier applied to features allows one to correct for experimental variations in cell brightness due to labeling. On dim cells (37% of standard brightness), with correction, we improved recall (49.26%→ 99.36%) without a significant drop in precision (99.99%→ 99.75%) . With tail vein injection, multipotent adult progenitor cells in a graft-versus-host-disease model in the first days post injection were predominantly found in lung, liver, spleen, and bone marrow. Distribution was not simply related to blood flow. The lung contained clusters of cells while other tissues contained single cells. Our methods provided stem cell distribution anywhere in mouse with single cell sensitivity. Methods should provide a rational means of evaluating dosing, delivery methods, cell enhancements, and mechanisms for therapeutic cells.
journal_name
IEEE Trans Med Imagingjournal_title
IEEE transactions on medical imagingauthors
Wuttisarnwattana P,Gargesha M,van't Hof W,Cooke KR,Wilson DLdoi
10.1109/TMI.2015.2497285subject
Has Abstractpub_date
2016-03-01 00:00:00pages
819-29issue
3eissn
0278-0062issn
1558-254Xjournal_volume
35pub_type
杂志文章abstract：:The authors describe a technique to reduce truncation artifacts in chemical shift images taken for in vivo magnetic resonance spectroscopy by using boundary information available from anatomic images. In this approach, high spatial frequency components are extrapolated from the observed data by making use of the bound...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/42.97578
更新日期：1991-01-01 00:00:00
abstract：:We propose a novel, physics-based method for detecting multi-scale tubular features in ultrasound images. The detector is based on a Hessian-matrix eigenvalue method, but unlike previous work, our detector is guided by an optimal model of vessel-like structures with respect to the ultrasound-image formation process. O...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2014.2340912
更新日期：2015-01-01 00:00:00
abstract：:The development of an automatic telemedicine system for computer-aided screening and grading of diabetic retinopathy depends on reliable detection of retinal lesions in fundus images. In this paper, a novel method for automatic detection of both microaneurysms and hemorrhages in color fundus images is described and va...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2015.2509785
更新日期：2016-04-01 00:00:00
abstract：:To solve the problem that traditional image registration methods based on continuous optimization for large motion lung 4D CT image sequences are easy to fall into local optimal solutions and lead to serious misregistration, a novel image registration method based on high-order Markov Random Field (MRF) is proposed. B...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2019.2937458
更新日期：2020-04-01 00:00:00
abstract：:A direct (noniterative) reconstruction algorithm for electrical impedance tomography in the two-dimensional (2-D), cross-sectional geometry is reviewed. New results of a reconstruction of a numerically simulated phantom chest are presented. The algorithm is based on the mathematical uniqueness proof by A. I. Nachman [...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2002.800574
更新日期：2002-06-01 00:00:00
abstract：:Magnetic resonance (MR) tagging has been shown to be a useful technique for noninvasively measuring the deformation of an in vivo heart. An important step in analyzing tagged images is the identification of tag lines in each image of a cine sequence. Most existing tag identification algorithms require user defined myo...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/42.768842
更新日期：1999-04-01 00:00:00
abstract：:Atherosclerosis at the carotid bifurcation resulting in cerebral emboli is a major cause of ischemic stroke. Most strokes associated with carotid atherosclerosis can be prevented by lifestyle/dietary changes and pharmacological treatments if identified early by monitoring carotid plaque changes. Registration of 3-D ul...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2008.918326
更新日期：2008-10-01 00:00:00
abstract：:High-resolution X-ray computed tomography (CT) imaging is routinely used for clinical pulmonary applications. Since lung function varies regionally and because pulmonary disease is usually not uniformly distributed in the lungs, it is useful to study the lungs on a lobe-by-lobe basis. Thus, it is important to segment ...
journal_title：IEEE transactions on medical imaging
pub_type： 临床试验,杂志文章
doi：10.1109/TMI.2005.859209
更新日期：2006-01-01 00:00:00
abstract：:This paper presents an algorithm to transform and reconstruct diffusion-weighted imaging (DWI) data for alignment of micro-structures in association with spatial transformations. The key idea is to decompose the diffusion signal profile, a function defined on a unit sphere, into a series of weighted diffusion basis fu...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2012.2204766
更新日期：2012-11-01 00:00:00
abstract：:In standard B-mode ultrasound (BMUS), segmentation of the lumen of atherosclerotic carotid arteries and studying the lumen geometry over time are difficult owing to irregular lumen shapes, noise, artifacts, and echolucent plaques. Contrast enhanced ultrasound (CEUS) improves lumen visualization, but lumen segmentation...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2014.2372784
更新日期：2015-04-01 00:00:00
abstract：:Deep tissue injuries are subcutaneous regions of tissue breakdown associated with excessive mechanical pressure for extended period of time. These wounds are currently clinically undetectable in their early stages and result in severe burdens on not only the patients who suffer from them, but the health care system as...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2014.2313082
更新日期：2014-07-01 00:00:00
abstract：:Tomographic reconstruction from positron emission tomography (PET) data is an ill-posed problem that requires regularization. An attractive approach is to impose an l(1) -regularization constraint, which favors sparse solutions in the wavelet domain. This can be achieved quite efficiently thanks to the iterative algor...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2008.923698
更新日期：2008-01-01 00:00:00
abstract：:This paper presents a statistical shape model for the automatic prostate segmentation in transrectal ultrasound images. A Gabor filter bank is first used to characterize the prostate boundaries in ultrasound images in both multiple scales and multiple orientations. The Gabor features are further reconstructed to be in...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2003.809057
更新日期：2003-04-01 00:00:00
abstract：:In magnetic resonance imaging (MRI), spatial resolution is limited by several factors such as acquisition time, short physiological phenomena, and organ motion. The acquired image usually has higher resolution in two dimensions (the acquisition plane) in comparison with the third dimension, resulting in highly anisotr...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2014.2329271
更新日期：2014-10-01 00:00:00
abstract：:An n-dimensional (n-D) filtered backprojection image reconstruction algorithm has been developed and used in the reconstruction of 4-D spectral-spatial magnetic resonance imaging (MRI) data. The algorithm uses n-1 successive stages of 2-D filtered backprojection to reconstruct an n-D image. This approach results in a ...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/42.387718
更新日期：1995-01-01 00:00:00
abstract：:A technique determines the optimal window width for orbiting rod transmission studies in positron emission tomography (PET). Windowing reduces noise in orbiting rod transmission studies. Lines-of-response (LOR) which intersect the rods generate primarily true coincidence events. LOR which pass far from the rods genera...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/42.387708
更新日期：1995-01-01 00:00:00
abstract：:The human cerebral cortex is topologically equivalent to a sphere when it is viewed as closed at the brain stem. Due to noise and/or resolution issues, magnetic resonance imaging may see "handles" that need to be eliminated to reflect the true spherical topology. Shattuck and Leahy present an algorithm to correct such...
journal_title：IEEE transactions on medical imaging
pub_type： 评论,信件
doi：10.1109/TMI.2002.806590
更新日期：2002-12-01 00:00:00
abstract：:New biopsy techniques, increased life expectancy, and prostate-specific antigen (PSA) screening have contributed to an increase in the reported incidence of prostate cancer. Among several treatment options available to the patients, transperineal prostate brachytherapy has emerged as a medically successful, cost-effec...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/42.736032
更新日期：1998-10-01 00:00:00
abstract：:Over the years, medical image tracking has gained considerable attention from both medical and research communities due to its widespread utility in a multitude of clinical applications, from functional assessment during diagnosis and therapy planning to structure tracking or image fusion during image-guided intervent...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2018.2840820
更新日期：2018-11-01 00:00:00
abstract：:This paper addresses the long object problem in helical cone-beam computed tomography. We present the PHI-method, a new algorithm for the exact reconstruction of a region-of-interest (ROI) of a long object from axially truncated data extending only slightly beyond the ROI. The PHI-method is an extension of the Radon-m...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/42.870247
更新日期：2000-05-01 00:00:00
abstract：:Photon counting computed tomography (PCCT) has the ability to identify individual photons, resulting in quantitative material identification. Meanwhile, several technical challenges still exist in current PCCT imaging systems, including increased noise and suboptimal bin selection. These nonideal effects can substanti...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2020.3001616
更新日期：2020-11-01 00:00:00
abstract：:Visual estimation of coronary obstruction severity from angiograms suffers from poor inter- and intraobserver reproducibility and is often inaccurate. In spite of the widely recognized limitations of visual analysis, automated methods have not found widespread clinical use, in part because they too frequently fail to ...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/42.370412
更新日期：1995-01-01 00:00:00
abstract：:In magnetic resonance imaging (MRI), it is well-known that patient motion plays a significant role in the degradation of image quality. Although the case of translational in-plane motion (x-y-motion) has been studied by several researchers, the effect of rigid, translational out-of-plane motion (z-motion) has not yet ...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/42.414611
更新日期：1995-01-01 00:00:00
abstract：:In Soares et al. (2000), the ensemble statistical properties of the rescaled block-iterative expectation-maximization (RBI-EM) reconstruction algorithm and rescaled block-iterative simultaneous multiplicative algebraic reconstruction technique (RBI-SMART) were derived. Included in this analysis were the special cases ...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/tmi.2004.836876
更新日期：2005-01-01 00:00:00
abstract：:Certain diseases cause permanent changes to the shapes and densities of nailfold capillaries and, therefore, nailfold capillaroscopy is important as a tool for diagnosing and monitoring these diseases. The first aim of the project is to resolve differences in terminology that have developed over the years in previous ...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/42.921482
更新日期：2001-04-01 00:00:00
abstract：:This paper presents a novel image-guided robot-based system to assist orthopedic surgeons in performing distal locking of long bone intramedullary nails. The system consists of a bone-mounted miniature robot fitted with a drill guide that provides rigid mechanical guidance for hand-held drilling of the distal screws' ...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2005.844922
更新日期：2005-05-01 00:00:00
abstract：:Three-dimensional (3-D) reconstructions of coronary bypass grafts performed from X-ray angiographic images may become increasingly important for the investigation of damaging mechanical stresses imposed to these vessels by the cyclic movement of the heart. Contrary to what we had experienced with coronary arteries, ap...
journal_title：IEEE transactions on medical imaging
pub_type： 信件
doi：10.1109/42.875203
更新日期：2000-07-01 00:00:00
abstract：:Ultrasound molecular imaging (USMI) is accomplished by detecting microbubble (MB) contrast agents that have bound to specific biomarkers, and can be used for a variety of imaging applications, such as the early detection of cancer. USMI has been widely utilized in preclinical imaging in mice; however, USMI in humans c...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2017.2774814
更新日期：2018-01-01 00:00:00
abstract：:High-resolution functional imaging of small animals is often obtained by single pinhole SPECT with circular orbit acquisition. Multipinhole SPECT adds information due to its improved sampling, and can improve the trade-off between resolution and sensitivity. To evaluate different pinhole collimator designs an efficien...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2007.902802
更新日期：2008-01-01 00:00:00
abstract：:Accurate localization of anatomical landmarks is an important step in medical imaging, as it provides useful prior information for subsequent image analysis and acquisition methods. It is particularly useful for initialization of automatic image analysis tools (e.g. segmentation and registration) and detection of scan...
journal_title：IEEE transactions on medical imaging
pub_type： 杂志文章
doi：10.1109/TMI.2016.2597270
更新日期：2017-01-01 00:00:00
