Abstract:
:N6-methyladenine (6mdA) is a widespread DNA modification in bacteria. More recently, 6mdA has also been characterized in mammalian DNA. However, measurements of 6mdA abundance and profiles are often very dissimilar between studies, even when performed on DNA from identical mammalian cell types. Using comprehensive bioinformatics analyses of published data and novel experimental approaches, we reveal that efforts to assay 6mdA in mammals have been severely compromised by bacterial contamination, RNA contamination, technological limitations, and antibody nonspecificity. These complications render 6mdA an exceptionally problematic DNA modification to study and have resulted in erroneous detection of 6mdA in several mammalian systems. Together, our results strongly imply that the evidence published to date is not sufficient to support the presence of 6mdA in mammals.
journal_name
Sci Advjournal_title
Science advancesauthors
Douvlataniotis K,Bensberg M,Lentini A,Gylemo B,Nestor CEdoi
10.1126/sciadv.aay3335subject
Has Abstractpub_date
2020-03-18 00:00:00pages
eaay3335issue
12issn
2375-2548pii
aay3335journal_volume
6pub_type
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