Abstract:
:Myofibroblasts orchestrate wound healing processes, and if they remain activated, they drive disease progression such as fibrosis and cancer. Besides growth factor signaling, the local extracellular matrix (ECM) and its mechanical properties are central regulators of these processes. It remains unknown whether transforming growth factor-β (TGF-β) and tensile forces work synergistically in up-regulating the transition of fibroblasts into myofibroblasts and whether myofibroblasts undergo apoptosis or become deactivated by other means once tissue homeostasis is reached. We used three-dimensional microtissues grown in vitro from fibroblasts in macroscopically engineered clefts for several weeks and found that fibroblasts transitioned into myofibroblasts at the highly tensed growth front as the microtissue progressively closed the cleft, in analogy to closing a wound site. Proliferation was up-regulated at the growth front, and new highly stretched fibronectin fibers were deposited, as revealed by fibronectin fluorescence resonance energy transfer probes. As the tissue was growing, the ECM underneath matured into a collagen-rich tissue containing mostly fibroblasts instead of myofibroblasts, and the fibronectin fibers were under reduced tension. This correlated with a progressive rounding of cells from the growth front inward, with decreased α-smooth muscle actin expression, YAP nuclear translocation, and cell proliferation. Together, this suggests that the myofibroblast phenotype is stabilized at the growth front by tensile forces, even in the absence of endogenously supplemented TGF-β, and reverts into a quiescent fibroblast phenotype already 10 μm behind the growth front, thus giving rise to a myofibroblast-to-fibroblast transition. This is the hallmark of reaching prohealing homeostasis.
journal_name
Sci Advjournal_title
Science advancesauthors
Kollmannsberger P,Bidan CM,Dunlop JWC,Fratzl P,Vogel Vdoi
10.1126/sciadv.aao4881subject
Has Abstractpub_date
2018-01-17 00:00:00pages
eaao4881issue
1issn
2375-2548pii
aao4881journal_volume
4pub_type
杂志文章相关文献
Science Advances文献大全abstract::Starvation is probably the most common stressful situation in nature. In vertebrates, elevation of the biogenic amine norepinephrine levels is common during starvation. However, the precise role of norepinephrine in nutrient deprivation remains largely unknown. We report that in the free-living nematode Caenorhabditis...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.1501372
更新日期:2016-05-06 00:00:00
abstract::Many animals understand numbers at a basic level for use in essential tasks such as foraging, shoaling, and resource management. However, complex arithmetic operations, such as addition and subtraction, using symbols and/or labeling have only been demonstrated in a limited number of nonhuman vertebrates. We show that ...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aav0961
更新日期:2019-02-06 00:00:00
abstract::About 40% of patients with non-small cell lung cancer (NSCLC) have stage IV cancer at the time of diagnosis. The only viable treatment options for metastatic disease are systemic chemotherapy and immunotherapy. Nonetheless, chemoresistance remains a major cause of chemotherapy failure. New immunotherapeutic modalities...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aba5542
更新日期:2020-06-17 00:00:00
abstract::We develop biohybrid magnetic microrobots by electrostatic self-assembly of nonmotile sperm cells and magnetic nanoparticles. Incorporating a biological entity into microrobots entails many functional advantages beyond shape templating, such as the facile uptake of chemotherapeutic agents to achieve targeted drug deli...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aba5855
更新日期:2020-07-08 00:00:00
abstract::Posthemorrhagic hydrocephalus (PHH) in premature infants is a common neurological disorder treated with invasive neurosurgical interventions. Patients with PHH lack effective therapeutic interventions and suffer chronic comorbidities. Here, we report a murine lysophosphatidic acid (LPA)-induced postnatal PHH model tha...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aax2011
更新日期:2019-10-09 00:00:00
abstract::Carbon deposits are well-known inhibitors of transition metal catalysts. In contrast to this undesirable behavior, here we show that epitaxial graphene grown on Ru(0001) promotes the reversible formation of a C-C bond between -CH2CN and 7,7,8,8-tetracyano-p-quinodimethane (TCNQ). The catalytic role of graphene is mult...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aau9366
更新日期:2018-12-14 00:00:00
abstract::Goat domestication was critical for agriculture and civilization, but its underlying genetic changes and selection regimes remain unclear. Here, we analyze the genomes of worldwide domestic goats, wild caprid species, and historical remains, providing evidence of an ancient introgression event from a West Caucasian tu...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaz5216
更新日期:2020-05-20 00:00:00
abstract::Triple-negative breast cancer (TNBC) is life-threatening because of limited therapies and lack of effective therapeutic targets. Here, we found that moesin (MSN) was significantly overexpressed in TNBC compared with other subtypes of breast cancer and was positively correlated with poor overall survival. However, litt...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaw9960
更新日期:2020-02-19 00:00:00
abstract::We investigate the emergence of global alignment in colonies of dividing rod-shaped cells under confinement. Using molecular dynamics simulations and continuous modeling, we demonstrate that geometrical anisotropies in the confining environment give rise to an imbalance in the normal stresses, which, in turn, drives a...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.abc8685
更新日期:2021-01-22 00:00:00
abstract::It has been proposed that Mars' moons formed from a disk produced by a large impact with the planet. However, whether such an event could produce tiny Phobos and Deimos remains unclear. Using a hybrid N-body model of moon accumulation that includes a full treatment of moon-moon dynamical interactions, we first identif...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aar6887
更新日期:2018-04-18 00:00:00
abstract::Moisture delivery to western North America is closely linked to variability in the westerly storm tracks of midlatitude cyclones, which are, in turn, modified by larger-scale features such as the El Niño-Southern Oscillation system. Instrumental and modeling data suggest that extratropical storm tracks may be intensif...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.1602263
更新日期:2017-06-07 00:00:00
abstract::A key problem in the study of the senses is to describe how sense organs extract perceptual information from the physics of the environment. We previously observed that dynamic touch elicits mechanical waves that propagate throughout the hand. Here, we show that these waves produce an efficient encoding of tactile inf...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaz1158
更新日期:2020-04-15 00:00:00
abstract::The Wiedemann-Franz (WF) law has been tested in numerous solids, but the extent of its relevance to the anomalous transverse transport and the topological nature of the wave function, remains an open question. Here, we present a study of anomalous transverse response in the noncollinear antiferromagnet Mn3Ge extended ...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaz3522
更新日期:2020-04-24 00:00:00
abstract::Sustainable structural materials with light weight, great thermal dimensional stability, and superb mechanical properties are vitally important for engineering application, but the intrinsic conflict among some material properties (e.g., strength and toughness) makes it challenging to realize these performance indexes...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaz1114
更新日期:2020-05-01 00:00:00
abstract::Wearable sweat analysis devices for monitoring of multiple health-related biomarkers with high sensitivity are highly desired for noninvasive and real-time monitoring of human health. Here, we report a flexible sweat analysis patch based on a silk fabric-derived carbon textile for simultaneous detection of six health-...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aax0649
更新日期:2019-11-08 00:00:00
abstract::Predator-prey interactions revealed by vertebrate trace fossils are extremely rare. We present footprint evidence from White Sands National Monument in New Mexico for the association of sloth and human trackways. Geologically, the sloth and human trackways were made contemporaneously, and the sloth trackways show evid...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aar7621
更新日期:2018-04-25 00:00:00
abstract::Cell lines are commonly used as cancer models. The tissue of origin provides context for understanding biological mechanisms and predicting therapy response. We therefore systematically examined whether cancer cell lines exhibit features matching the presumed cancer type of origin. Gene expression and DNA methylation ...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aba1862
更新日期:2020-07-01 00:00:00
abstract::Present-day dermatological diagnostic tools are expensive, time-consuming, require substantial operational expertise, and typically probe only the superficial layers of skin (~15 μm). We introduce a soft, battery-free, noninvasive, reusable skin hydration sensor (SHS) adherable to most of the body surface. The platfor...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.abd7146
更新日期:2020-12-04 00:00:00
abstract::Lymphatic filariasis and onchocerciasis are two important neglected tropical diseases (NTDs) that cause severe disability. Control efforts are hindered by the lack of a safe macrofilaricidal drug. Targeting the Wolbachia bacterial endosymbionts in these parasites with doxycycline leads to a macrofilaricidal outcome, b...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aao1551
更新日期:2017-09-27 00:00:00
abstract::Recent research on piezoelectric materials is predominantly devoted to enhancing the piezoelectric coefficient, but overlooks its sign, largely because almost all of them exhibit positive longitudinal piezoelectricity. The only experimentally known exception is ferroelectric polymer poly(vinylidene fluoride) and its c...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aav3780
更新日期:2019-04-19 00:00:00
abstract::Fluctuations in biodiversity, large and small, pervade the fossil record, yet we do not understand the processes generating them. Here, we extend theory from nonequilibrium statistical physics to describe the fat-tailed form of fluctuations in Phanerozoic marine invertebrate richness. Using this theory, known as super...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aat0122
更新日期:2019-06-26 00:00:00
abstract::Materials with a stochastic microstructure, like foams, typically exhibit low mechanical stiffness, whereas lattices with a designed microarchitecture often show notably improved stiffness. These periodic architected materials have previously been designed by rule, using the Maxwell criterion to ensure that their defo...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaw1937
更新日期:2019-09-27 00:00:00
abstract::Electrochemical analysis of sweat using soft bioelectronics on human skin provides a new route for noninvasive glucose monitoring without painful blood collection. However, sweat-based glucose sensing still faces many challenges, such as difficulty in sweat collection, activity variation of glucose oxidase due to lact...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.1601314
更新日期:2017-03-08 00:00:00
abstract::Photoresponsive liquid crystalline elastomers (LCEs) constitute ideal actuators for soft robots because their light-induced macroscopic shape changes can be harnessed to perform specific articulated motions. Conventional LCEs, however, do not typically exhibit complex modes of bending and twisting necessary to perform...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aay5349
更新日期:2020-03-27 00:00:00
abstract::Behavioral and clinical studies suggest a critical role of substance P (SP)/neurokinin-1 receptor (NK-1R) signaling in alcohol dependence. Here, we examined regulation of GABA transmission in the medial subdivision of the central amygdala (CeM) by the SP/NK-1R system, and its neuroadaptation following chronic alcohol ...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaz1050
更新日期:2020-03-18 00:00:00
abstract::By developing new computational models, we examine how enzymatic reactions on an underlying surface can be harnessed to direct the motion and organization of reagent-laden microcapsules in a fluid-filled microchannel. In the presence of appropriate reagents, surface-bound enzymes can act as pumps, which drive large-sc...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.1501835
更新日期:2016-03-18 00:00:00
abstract::Forecasting fields of oceanic phenomena has long been dependent on physical equation-based numerical models. The challenge is that many natural processes need to be considered for understanding complicated phenomena. In contrast, rules of the processes are already embedded in the time-series observation itself. Thus, ...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aba1482
更新日期:2020-07-15 00:00:00
abstract::One of the most plausible scenarios of the origin of life assumes the preceding prebiotic autotrophic metabolism in sulfide-rich hydrothermal vent environments. However, geochemical mechanisms to harness the reductive power provided by hydrothermal systems remain to be elucidated. Here, we show that, under a geoelectr...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aav7848
更新日期:2019-06-19 00:00:00
abstract::In addition to their conventional uses, metal-organic frameworks (MOFs) have recently emerged as an interesting class of functional materials and precursors of inorganic materials for electrochemical energy storage and conversion technologies. This class of MOF-related materials can be broadly categorized into two gro...
journal_title:Science advances
pub_type: 杂志文章,评审
doi:10.1126/sciadv.aap9252
更新日期:2017-12-01 00:00:00
abstract::The retinoic acid-inducible gene I (RIG-I) innate immune receptor is an important immunotherapeutic target, but we lack approaches for monitoring the physical basis for its activation in vitro. This gap in our understanding has led to confusion about mechanisms of RIG-I activation and difficulty discovering agonists a...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aax3641
更新日期:2019-10-02 00:00:00