Abstract:
:Self-propelling micromotors are emerging as a promising micro- and nanoscale tool for single-cell analysis. We have recently shown that the field gradients necessary to manipulate matter via dielectrophoresis can be induced at the surface of a polarizable active ("self-propelling") metallodielectric Janus particle (JP) under an externally applied electric field, acting essentially as a mobile floating microelectrode. Here, we successfully demonstrated that the application of an external electric field can singularly trap and transport bacteria and can selectively electroporate the trapped bacteria. Selective electroporation, enabled by the local intensification of the electric field induced by the JP, was obtained under both continuous alternating current and pulsed signal conditions. This approach is generic and applicable to bacteria and JP, as well as a wide range of cell types and micromotor designs. Hence, it constitutes an important and novel experimental tool for single-cell analysis and targeted delivery.
journal_name
Sci Advjournal_title
Science advancesauthors
Wu Y,Fu A,Yossifon Gdoi
10.1126/sciadv.aay4412subject
Has Abstractpub_date
2020-01-29 00:00:00pages
eaay4412issue
5issn
2375-2548pii
aay4412journal_volume
6pub_type
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