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Self-assembly formed by a short DNA probe pair: Application for highly sensitive mRNA species detection without reverse transcription.

Abstract：

:We describe a novel technology for detecting nucleic acids: Probe Alteration Link Self-Assembly Reactions (PALSAR). PALSAR comprises DNA self-assembly of pairs of short DNA probes formed by alternate hybridization of three complementary regions in a pair of honeycomb probes (HCPs). Self-assembly occurs at designated salt concentrations and reaction temperatures and requires no enzymes. We prepared pairs of HCPs to detect mRNAs encoded by the GAPDH gene β-actin (BA) gene, CD3D gene, CD4 gene, major vault protein (MV) gene and the signalling lymphocytic activation molecule-associated protein (SAP) gene, and succeeded in quantitatively detecting these mRNAs. PALSAR could detect mRNA directly without synthesizing cDNA. Moreover, multiple mRNAs could be detected simultaneously in a single reaction tube and there was a good correlation between the results obtained PALSAR and those by real-time PCR.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Usui M,Fujikawa T,Osawa M,Hakii C,Ikumi N,Nozaki T,Kitamura N,Hatta Y,Fujiwara S,Takei M

doi

10.1016/j.bbrc.2015.10.019

subject

Has Abstract

pub_date

2015-11-27 00:00:00

pages

1012-8

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(15)30723-3

journal_volume

467

pub_type

杂志文章

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