Abstract：
:The engineered ascorbate peroxidase (APEX) is a powerful tool for the proximity-dependent labeling of proteins and RNAs in live cells. Although widely use in mammalian cells, APEX applications in microorganisms have been hampered by the poor labeling efficiency of its biotin-phenol (BP) substrate. In this study, we sought to address this challenge by designing and screening a panel of alkyne-functionalized substrates. Our best probe, Alk-Ph, substantially improves APEX-labeling efficiency in intact yeast cells, as it is more cell wall-permeant than BP. Through a combination of protein-centric and peptide-centric chemoproteomic experiments, we have identified 165 proteins with a specificity of 94% in the yeast mitochondrial matrix. In addition, we have demonstrated that Alk-Ph is useful for proximity-dependent RNA labeling in yeast, thus expanding the scope of APEX-seq. We envision that this improved APEX-labeling strategy would set the stage for the large-scale mapping of spatial proteome and transcriptome in yeast.
journal_name
Cell Chem Bioljournal_title
Cell chemical biologyauthors
Li Y,Tian C,Liu K,Zhou Y,Yang J,Zou Pdoi
10.1016/j.chembiol.2020.05.006subject
Has Abstractpub_date
2020-07-16 00:00:00pages
858-865.e8issue
7eissn
2451-9456issn
2451-9448pii
S2451-9456(20)30152-5journal_volume
27pub_type
杂志文章abstract：:A protein-fragment complementation assay (PCA) for detecting and localizing intracellular protein-protein interactions (PPIs) was built by bisection of miniSOG, a fluorescent flavoprotein derived from the light, oxygen, voltage (LOV)-2 domain of Arabidopsis phototropin. When brought together by interacting proteins, t...
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journal_title：Cell chemical biology
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journal_title：Cell chemical biology
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pub_type： 杂志文章,评审
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journal_title：Cell chemical biology
pub_type： 杂志文章
doi：10.1016/j.chembiol.2018.06.013
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journal_title：Cell chemical biology
pub_type： 杂志文章
doi：10.1016/j.chembiol.2017.09.007
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journal_title：Cell chemical biology
pub_type： 杂志文章
doi：10.1016/j.chembiol.2019.07.004
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journal_title：Cell chemical biology
pub_type： 评论,杂志文章
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journal_title：Cell chemical biology
pub_type： 杂志文章
doi：10.1016/j.chembiol.2019.04.009
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journal_title：Cell chemical biology
pub_type： 杂志文章
doi：10.1016/j.chembiol.2016.11.007
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journal_title：Cell chemical biology
pub_type： 评论,杂志文章
doi：10.1016/j.chembiol.2016.05.003
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journal_title：Cell chemical biology
pub_type： 杂志文章,评审
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journal_title：Cell chemical biology
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journal_title：Cell chemical biology
pub_type： 杂志文章
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journal_title：Cell chemical biology
pub_type： 杂志文章
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journal_title：Cell chemical biology
pub_type： 杂志文章
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journal_title：Cell chemical biology
pub_type： 杂志文章
doi：10.1016/j.chembiol.2019.04.008
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journal_title：Cell chemical biology
pub_type： 杂志文章
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journal_title：Cell chemical biology
pub_type： 杂志文章
doi：10.1016/j.chembiol.2016.10.006
更新日期：2016-11-17 00:00:00
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journal_title：Cell chemical biology
pub_type： 杂志文章
doi：10.1016/j.chembiol.2018.07.009
更新日期：2018-11-15 00:00:00
abstract：:Pyridoxal 5'-phosphate (PLP) is a versatile cofactor that catalyzes a plethora of chemical transformations within a cell. Although many human PLP-dependent enzymes (PLP-DEs) with crucial physiological and pathological roles are known, a global method enabling their cellular profiling is lacking. Here, we demonstrate t...
journal_title：Cell chemical biology
pub_type： 杂志文章
doi：10.1016/j.chembiol.2019.08.003
更新日期：2019-10-17 00:00:00
