Peptidomics for the discovery and characterization of neuropeptides and hormones.

Abstract:

:The discovery of neuropeptides as signaling molecules with paracrine or hormonal regulatory functions has led to trailblazing advances in physiology and fostered the characterization of numerous neuropeptide-binding G protein-coupled receptors (GPCRs) as potential drug targets. The impact on human health has been tremendous: approximately 30% of commercial drugs act via the GPCR pathway. However, about 25% of the GPCRs encoded by the mammalian genome still lack their pharmacological identity. Searching for the orphan GPCR endogenous ligands that are likely to be neuropeptides has proved to be a formidable task. Here we describe the mass spectrometry (MS)-based technologies and experimental strategies that have been successful in achieving high-throughput characterization of endogenous peptides in nervous and endocrine systems.

journal_name

Trends Pharmacol Sci

authors

Romanova EV,Sweedler JV

doi

10.1016/j.tips.2015.05.009

subject

Has Abstract

pub_date

2015-09-01 00:00:00

pages

579-86

issue

9

eissn

0165-6147

issn

1873-3735

pii

S0165-6147(15)00115-7

journal_volume

36

pub_type

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