A buprenorphine-validated rat model of opioid use disorder optimized to study sex differences in vulnerability to relapse.


RATIONALE:Opioid use disorder (OUD) is a major epidemic in the USA. Despite evidence indicating that OUD may be particularly severe for women, preclinical models have yet to establish sex as a major factor in OUD. OBJECTIVES:Here, we examined sex differences in vulnerability to relapse following intermittent access fentanyl self-administration and protracted abstinence and used buprenorphine, the FDA-approved treatment for OUD, to test the validity of our model. METHODS:Following acquisition of fentanyl self-administration under one of two training conditions, male and female rats were given extended, 24-h/day access to fentanyl (0.25 μg/kg/infusion, 10 days) using an intermittent access procedure. Vulnerability to relapse was assessed using an extinction/cue-induced reinstatement procedure following 14 days of abstinence; buprenorphine (0 or 3 mg/kg/day) was administered throughout abstinence. RESULTS:Levels of drug-seeking were high following extended-access fentanyl self-administration and abstinence; buprenorphine markedly decreased drug-seeking supporting the validity of our relapse model. Females self-administered more fentanyl and responded at higher levels during subsequent extinction testing. Buprenorphine was effective in both sexes and eliminated sex and estrous phase differences in drug-seeking. Interestingly, the inclusion of a time-out during training had a major impact on later fentanyl self-administration in females, but not males, indicating that the initial exposure conditions can persistently impact vulnerability in females. CONCLUSIONS:These findings demonstrate the utility of this rat model for determining sex and hormonal influences on the development and treatment of OUD.




Bakhti-Suroosh A,Towers EB,Lynch WJ




Has Abstract


2021-01-06 00:00:00








  • Differences in the effects of d-fenfluramine and morphine on various responses of rats to painful stimuli.

    abstract::The effects of d-fenfluramine and morphine on various nociceptive responses of rats were investigated. Unlike morphine, which inhibited all the responses examined, d-fenfluramine inhibited jumping and paw licking of rats on a hot plate, but did not increase the latency of tail withdrawal from hot water. The effects of...


    pub_type: 杂志文章


    authors: Rochat C,Cervo L,Romandini S,Samanin R

    更新日期:1982-01-01 00:00:00

  • Tolerance to the anti-pentylenetetrazole effects of diazepam in the mouse.

    abstract::The protective effects of acute and chronic diazepam administration (4 mg/kg) against seizures induced by pentylenetetrazole (PTZ) and picrotoxin were investigated. Considering the incidence of tonic-clonic convulsions, tolerance to the protective effects of diazepam was evident by day 5 if the mice were challenged wi...


    pub_type: 杂志文章


    authors: File SE

    更新日期:1983-01-01 00:00:00

  • Temporal factors affecting cocaine-opioid interactions: a cocaine drug discrimination study in rats.

    abstract:RATIONALE:Increasing concomitant abuse of cocaine and morphine-like opioids has prompted a number of studies aimed at understanding how these drugs interact. OBJECTIVE:The present study was designed to determine if variations in opioid pretreatment time would affect how mu opioid agonists interact with cocaine. METHO...


    pub_type: 杂志文章


    authors: Green-Jordan K,Warren L,Kantak KM

    更新日期:2001-08-01 00:00:00

  • Effects of caffeine on discrimination learning, consolidation, and learned behavior in mice.

    abstract::Three sets of experiments were carried out in a Y water maze in which mice had to swim toward the light or the dark, in two different procedures. The first procedure involved, orientation toward a light source (L procedure), corresponding to natural preference, the latter involved orientation toward the dark (D proced...


    pub_type: 杂志文章


    authors: Castellano C

    更新日期:1976-08-17 00:00:00

  • Effector antagonism by the regulators of G protein signalling (RGS) proteins causes desensitization of mu-opioid receptors in the CNS.

    abstract:RATIONALE:In cell culture systems, agonists can promote the phosphorylation and internalization of receptors coupled to G proteins (GPCR), leading to their desensitization. However, in the CNS opioid agonists promote a profound desensitization of their analgesic effects without diminishing the presence of their recepto...


    pub_type: 杂志文章,评审


    authors: Garzón J,Rodríguez-Muñoz M,de la Torre-Madrid E,Sánchez-Blázquez P

    更新日期:2005-06-01 00:00:00

  • Preclinical studies of stress, extinction, and prefrontal cortex: intriguing leads and pressing questions.

    abstract:BACKGROUND:Stress is associated with cognitive and emotional dysfunction, and increases risk for a variety of psychological disorders, including depression and posttraumatic stress disorder. Prefrontal cortex is critical for executive function and emotion regulation, is a target for stress hormones, and is implicated i...


    pub_type: 杂志文章,评审


    authors: Wellman CL,Moench KM

    更新日期:2019-01-01 00:00:00

  • Early effects of mirtazapine on emotional processing.

    abstract:BACKGROUND:Acute administration of selective serotonin and noradrenaline re-uptake blockers to healthy volunteers affects the processing of emotional information but it is not known if similar effects occur with antidepressants acting through other pharmacological mechanisms. Mirtazapine is a clinically established ant...


    pub_type: 杂志文章,随机对照试验


    authors: Arnone D,Horder J,Cowen PJ,Harmer CJ

    更新日期:2009-05-01 00:00:00

  • High-dose methadone produces superior opioid blockade and comparable withdrawal suppression to lower doses in opioid-dependent humans.

    abstract:RATIONALE:The efficacy of methadone for treating heroin dependence derives, in part, from suppression of opiate withdrawal and attenuation of the effects of heroin. OBJECTIVES:The purpose of this double-blind, within-subject, inpatient study was to determine whether larger doses of methadone, which are more effective ...


    pub_type: 杂志文章


    authors: Donny EC,Walsh SL,Bigelow GE,Eissenberg T,Stitzer ML

    更新日期:2002-05-01 00:00:00

  • Pentagastrin has panic-inducing properties in obsessive compulsive disorder.

    abstract::The effects of the CCKB-receptor agonist pentagastrin, a synthetic analogue of the cholecystokinin tetrapeptide (CCK-4), were studied in seven patients suffering from obsessive compulsive disorder (OCD) and seven healthy controls. All subjects were challenged with an IV dose of 0.6 micrograms/kg pentagastrin or placeb...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: de Leeuw AS,Den Boer JA,Slaap BR,Westenberg HG

    更新日期:1996-08-01 00:00:00

  • Interactions between social stress and morphine in the periaqueductal gray: effects on affective vocal and reflexive pain responses in rats.

    abstract:RATIONALE:Endogenous opioid systems within the mesencephalic periaqueductal gray matter (PAG) appear to be intricately involved in many affective, defensive, submissive, and reflexive responses, and these systems are activated by aversive stimuli. OBJECTIVES:The present experiments evaluated the influence of opioid re...


    pub_type: 杂志文章


    authors: Vivian JA,Miczek KA

    更新日期:1999-09-01 00:00:00

  • Effects of chlordiazepoxide and diazepam on feeding performance in a food-preference test.

    abstract::Chlordiazepoxide (5 and 10 mg/kg) and diazepam (2.5 mg/kg) reduced the latency to eat and enhanced feeding response to familiar food in a food-preference test. The increased feeding response resulted from an increased frequency of individual eating episodes (bouts) without significant change in the episode duration. E...


    pub_type: 杂志文章


    authors: Cooper SJ

    更新日期:1980-01-01 00:00:00

  • Effects of acute selective 5-HT1, 5-HT2, 5-HT3 receptor and alpha 2 adrenoceptor blockade on naloxone-induced antinociception.

    abstract::Several studies have demonstrated a paradoxical form of antinociception induced by the repeated administration of opioid antagonists accompanied by exposure to a painful stimulus. The underlying mechanism of this naloxone-induced antinociception (NIA) is still unknown, but the results of several studies suggest that i...


    pub_type: 杂志文章


    authors: Walker MJ,Poulos CX,Le AD

    更新日期:1994-01-01 00:00:00

  • Neuroleptic properties of cis-N-(1-benzyl-2-methylpyrrolidin-3-yl)-5-chloro-2-methoxy-4-methylaminobenzamide (YM-09151-2) with selective antidopaminergic activity.

    abstract::A new benzamide, cis-N-(1-benzyl-2-methylpyrrolidin - 3 - yl) - 5 - chloro - 2 - methoxy - 4 - methylaminobenzamide (YM-09151-2) exhibited more potent and longer-lasting inhibitory effects on apomorphine-induced behaviours (stereotyped behaviour, emesis and hypothermia), and methamphetamine-induced stereotyped behavio...


    pub_type: 杂志文章


    authors: Usuda S,Nishikori K,Noshiro O,Maeno H

    更新日期:1981-01-01 00:00:00

  • The dopamine D2 receptor antagonist sulpiride modulates striatal BOLD signal during the manipulation of information in working memory.

    abstract:RATIONALE:Dopamine (DA) plays an important role in working memory. However, the precise functions supported by different DA receptor subtypes in different neural regions remain unclear. OBJECTIVE:The present study used pharmacological, event-related fMRI to test the hypothesis that striatal dopamine is important for t...


    pub_type: 临床试验,杂志文章


    authors: Dodds CM,Clark L,Dove A,Regenthal R,Baumann F,Bullmore E,Robbins TW,Müller U

    更新日期:2009-11-01 00:00:00

  • Serotonin and tolerance to delay of reward in rats.

    abstract:RATIONALE:Tolerance to delay of gratification, taken to reflect impulsiveness, has been proposed to be under the preferential control of central serotonin (5-HT) processes. OBJECTIVE:The present study further examined the effects of drugs which directly or indirectly alter 5-HT transmission, on behaviour controlled by...


    pub_type: 杂志文章


    authors: Bizot J,Le Bihan C,Puech AJ,Hamon M,Thiébot M

    更新日期:1999-10-01 00:00:00

  • Noradrenergic receptor mechanisms in neophobia.

    abstract::We have previously demonstrated that depletion of forebrain norepinephrine (NE) led to an attenuation of neophobia in a novel environment, as defined by a greater preference for novel food over familiar food. To study further the role of forebrain NE in neophobia we chronically infused noradrenergic receptor ligands o...


    pub_type: 杂志文章


    authors: Steketee JD,Silverman PB,Swann AC

    更新日期:1992-01-01 00:00:00

  • Specific oculomotor deficit after acute methadone. II. Smooth pursuit eye movements.

    abstract::Changes in smooth pursuit eye tracking of targets moving sinusoidally in horizontal and vertical planes before and after up to 10 mg oral methadone were measured electrooculographically in nontolerant nondependent humans. Methadone depressed the gain of horizontal tracking movements at most frequencies tested (0.2 - 1...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Rothenberg S,Schottenfeld S,Selkoe D,Gross K

    更新日期:1980-01-01 00:00:00

  • Hypnotic susceptibility to various depressants in rats selected for differential ethanol sensitivity.

    abstract::MA rats, bred for greater motor impairment following subhypnotic doses of ethanol, were found to be more sensitive to the hypnotic effects of phenobarbital and chloral hydrate than were LA rats. In addition, the previously reported finding of a difference between the two lines of rats in duration of loss of righting r...


    pub_type: 杂志文章


    authors: Riley EP,Shapiro NR,Lochry EA

    更新日期:1979-02-28 00:00:00

  • Differential effects of dopamine D1 and D 2/3 receptor antagonism on motor responses.

    abstract:RATIONALE:The zebrafish dopaminergic system is thought to be evolutionarily conserved and may be amenable to pharmacological manipulation using drugs developed for mammalian receptors. However, only few studies have examined the role of specific receptor subtypes in behaviour of adult zebrafish. OBJECTIVES:The objecti...


    pub_type: 杂志文章


    authors: Tran S,Nowicki M,Muraleetharan A,Gerlai R

    更新日期:2015-02-01 00:00:00

  • Effects of naloxone and naltrexone on the increased water intake and drinking duration in phenobarbitone-treated rats.

    abstract::In two experiments, phenobarbitone sodium (30 mg/kg) reliably enhanced water consumption and extended the duration of drinking in 24h water-deprived male rats. The opiate receptor antagonists naloxone (0.1-10 mg/kg) and naltrexone (0.1-10 mg/kg) both decreased water intake and reduced the duration of drinking. When th...


    pub_type: 杂志文章


    authors: Cooper SJ,McGivern H

    更新日期:1983-01-01 00:00:00

  • Tolerance to morphine stimulus control: role of morphine maintenance dose.

    abstract::Experiments assessed the development of tolerance to morphine stimulus control during treatment with selected maintenance doses of morphine. Separate groups of rats were trained to discriminate saline and either 3.2 mg/kg or 5.6 mg/kg morphine under fixed-ratio schedules of food delivery. Dose-response functions for g...


    pub_type: 杂志文章


    authors: Young AM,Sannerud CA,Steigerwald ES,Doty MD,Lipinski WJ,Tetrick LE

    更新日期:1990-01-01 00:00:00

  • CD-1 and Balb/cJ mice do not show enduring antidepressant-like effects of ketamine in tests of acute antidepressant efficacy.

    abstract:RATIONALE:In patients, ketamine is a fast-acting antidepressant that can induce long-lasting symptom relief. Similar rapid effects have been reported in rodents, but reports of lasting effects are limited. OBJECTIVES:We sought to extend past findings by examining dose-response curves that overlap with the individual d...


    pub_type: 杂志文章


    authors: Bechtholt-Gompf AJ,Smith KL,John CS,Kang HH,Carlezon WA Jr,Cohen BM,Ongür D

    更新日期:2011-06-01 00:00:00

  • Comparison of measured and FTC-predicted nicotine uptake in smokers.

    abstract::Cigarette smokers have a wide variety of "tar" and nicotine yields to choose from in the current market, ranging from 0.5 mg "tar" and less than 0.05 mg nicotine to 27 mg "tar" and 1.8 mg nicotine by the Federal Trade Commission (FTC) method. To understand better the relationship between FTC nicotine yields and actual...


    pub_type: 临床试验,杂志文章


    authors: Byrd GD,Robinson JH,Caldwell WS,deBethizy JD

    更新日期:1995-11-01 00:00:00

  • Mood responses of remitted schizophrenics to methylphenidate infusion.

    abstract::To investigate the mood response of schizophrenic subjects to psychostimulant challenge, 29 neuroleptic-treated subjects (22 with schizophrenia and 7 with schizoaffective disorder) in clinical remission received two infusions, one with methylphenidate 0.5 mg/kg and the other with normal saline, under double-blind cond...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Robinson D,Mayerhoff D,Alvir J,Cooper T,Lieberman J

    更新日期:1991-01-01 00:00:00

  • Hold-down as an alternative to unit dose in cocaine self-administration experiments: Characterization using a progressive ratio schedule.

    abstract:RATIONALE:Virtually all cocaine self-administration studies have used a "unit dose" as a reinforcing stimulus; the subject is a passive recipient of an experimenter-selected dose. OBJECTIVES:The present experiments examined the consequence of requiring the subject to actively determine the dose and speed of each injec...


    pub_type: 杂志文章


    authors: Roberts DCS,Zimmer BA

    更新日期:2020-09-01 00:00:00

  • The effect of reboxetine co-administration with olanzapine on metabolic and endocrine profile in schizophrenia patients.

    abstract:RATIONALE:We previously demonstrated that the addition of the selective norepinephrine reuptake inhibitor reboxetine attenuates olanzapine-induced weight gain. Using the same study sample, we also sought to determine whether reboxetine's weight-attenuating effect was accompanied by a beneficial effect on metabolic and ...


    pub_type: 杂志文章,随机对照试验


    authors: Amrami-Weizman A,Maayan R,Gil-Ad I,Pashinian A,Fuchs C,Kotler M,Poyurovsky M

    更新日期:2013-11-01 00:00:00

  • Effects of venlafaxine in the sleep architecture of rats.

    abstract::Different venlafaxine doses (1, 5, and 10 mg/kg) and saline solution were administered to ten male Wistar rats (Latin-Square design). Compared with saline, venlafaxine produced a dose-related suppression of REM sleep and an increase in wake time while slow wave sleep was reduced. This effect is similar to the one that...


    pub_type: 杂志文章


    authors: Salin-Pascual RJ,Moro-Lopez ML

    更新日期:1997-02-01 00:00:00

  • Behavioural and biochemical effects of tryptophan, tyrosine and phenylalanine in mice.

    abstract::Behaviour was observed in mice given L-tryptophan, L-tyrosine or L-phenylalanine and then placed either in an open field or in water in a narrow cylinder. Animals placed in water (swim test) soon assume a characteristic immobile posture. Most antidepressants, including pharmacologically atypical ones, decrease immobil...


    pub_type: 杂志文章


    authors: Gibson CJ,Deikel SM,Young SN,Binik YM

    更新日期:1982-01-01 00:00:00

  • Opioid receptor subtype-specific cross-tolerance to the effects of morphine on schedule-controlled behavior in mice.

    abstract::Key-press responding of mice was maintained under a fixed-ratio (FR) 30-response schedule of food presentation. Successive 3-min periods during which the experimental chamber was illuminated and the schedule was in effect were preceded by 10-min time-out (TO) periods during which all lights were out and responses had ...


    pub_type: 杂志文章


    authors: Solomon RE,Goodrich JE,Katz JL

    更新日期:1988-01-01 00:00:00

  • A novel 2- and 3-choice touchscreen-based continuous trial-unique nonmatching-to-location task (cTUNL) sensitive to functional differences between dentate gyrus and CA3 subregions of the hippocampus.

    abstract:RATIONALE:The touchscreen continuous trial-unique non-matching-to-location task (cTUNL) has been developed to optimise a battery of tasks under NEWMEDS (Novel Methods leading to New Medication in Depression and Schizophrenia, http://www.newmeds-europe.com ). It offers novel task features of both a practical and a theor...


    pub_type: 杂志文章


    authors: Oomen CA,Hvoslef-Eide M,Kofink D,Preusser F,Mar AC,Saksida LM,Bussey TJ

    更新日期:2015-11-01 00:00:00