devR PCR for the diagnosis of intraocular tuberculosis.

Abstract:

PURPOSE:To compare the efficacy of devR and MPB64 PCR in the diagnosis of intraocular tuberculosis. METHODS:Prospective, nonrandomized study. Seventy-five patients were enrolled in 3 groups. Group A had 25 patients with presumed intraocular tubercular uveitis, group B had 25 controls with specific uveitis other than tubercular uveitis, and group C included 25 non-uveitic negative controls. The undiluted vitreous/aqueous samples were collected and subjected to PCR assay for devR and MPB64 gene sequence of Mycobacterium tuberculosis (MTB) to detect sensitivity and specificity. RESULTS:devR PCR was positive in 16 (64%) out of 25 patients with presumed tubercular uveitis. MPB64 PCR was positive in 18 (72%) out of 25 patients with presumed tubercular uveitis. The sensitivity and specificity of devR were 64 and 100%, respectively. The sensitivity and specificity of MPB64 PCR were 72 and 100%, respectively. CONCLUSION:devR PCR is not a better tool than MPB64 PCR for diagnosing intraocular tuberculosis.

journal_name

Ocul Immunol Inflamm

authors

Kataria P,Kumar A,Bansal R,Sharma A,Gupta V,Gupta A,Singh R,Sharma K

doi

10.3109/09273948.2014.981550

subject

Has Abstract

pub_date

2015-02-01 00:00:00

pages

47-52

issue

1

eissn

0927-3948

issn

1744-5078

journal_volume

23

pub_type

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