Abstract:
:A simple procedure has been developed for the large scale purification of fetal bovine serum acetylcholinesterase (AChE) (EC 3.1.1.7). The procedure involves two steps: batch adsorption of the AChE from 250 L of serum onto a procainamide affinity Sepharose 4B gel; and analytical procainamide affinity chromatography of the step-1 product. Over 100 mg of AChE was purified in 10 days to apparent homogeneity with this procedure.
journal_name
Life Scijournal_title
Life sciencesauthors
De la Hoz D,Doctor BP,Ralston JS,Rush RS,Wolfe ADdoi
10.1016/0024-3205(86)90530-8subject
Has Abstractpub_date
1986-07-21 00:00:00pages
195-9issue
3eissn
0024-3205issn
1879-0631pii
0024-3205(86)90530-8journal_volume
39pub_type
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