Abstract:
:Biological properties of homogeneous solutions of chicken (c) and mammalian (m) LHRH were compared by their ability to release LH, in vitro, from a rooster pituitary cell incubation system. Homogeneity of the two LHRH species was confirmed by High Performance Liquid Chromatography (HPLC) using linear gradients of acetonitrile and phosphate buffer. A clear HPLC separation of [Gln8]-LHRH ( cLHRH ) and [Arg8]-LHRH ( mLHRH ) was obtained, with the former having a consistently longer retention time than the latter. cLHRH cause a greater (p less than .025) in vitro release of LH at low doses (less than 1 ng/2 X 10(5) live pituitary cells), but not at high doses (greater than 10 ng/2 X 10(5) live pituitary cells), than that caused by mLHRH . Our results indicate that rooster pituitary cells are significantly more sensitive to low doses of cLHRH than to similar doses of mLHRH , when assessed by their ability to release LH in vitro.
journal_name
Life Scijournal_title
Life sciencesauthors
Johnson AL,Dickerman RW,Advis JPdoi
10.1016/0024-3205(84)90678-7subject
Has Abstractpub_date
1984-05-07 00:00:00pages
1847-51issue
19eissn
0024-3205issn
1879-0631pii
0024-3205(84)90678-7journal_volume
34pub_type
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