Abstract:
:A fluorometric assay is described for the detection of red blood cell antibodies. The assay reveals as little as 600 molecules of bound, fluoresceinated rabbit anti-human IgG antibodies per erythrocyte. Eleven patients with possible autoimmune erythrocyte disorder and negative direct antiglobulin test were studied by the fluorometric assay. The outcome of the fluorometric assay was compared with that of the human allogeneic rosette test. Results obtained by the two methods were in complete agreement. Five of the patients were shown to possess unexpectedly high levels of erythrocyte-bound IgG in spite of a negative, direct antiglobulin test. These findings and the validity of the fluorometric assay are discussed.
journal_name
Transfusionjournal_title
Transfusionauthors
Schreiber AB,Lambermont M,Strosberg AD,Wybran Jdoi
10.1046/j.1537-2995.1981.21281178153.xsubject
Has Abstractpub_date
1981-03-01 00:00:00pages
178-83issue
2eissn
0041-1132issn
1537-2995journal_volume
21pub_type
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