A fluorometric assay for red blood cell antibodies.

Abstract:

:A fluorometric assay is described for the detection of red blood cell antibodies. The assay reveals as little as 600 molecules of bound, fluoresceinated rabbit anti-human IgG antibodies per erythrocyte. Eleven patients with possible autoimmune erythrocyte disorder and negative direct antiglobulin test were studied by the fluorometric assay. The outcome of the fluorometric assay was compared with that of the human allogeneic rosette test. Results obtained by the two methods were in complete agreement. Five of the patients were shown to possess unexpectedly high levels of erythrocyte-bound IgG in spite of a negative, direct antiglobulin test. These findings and the validity of the fluorometric assay are discussed.

journal_name

Transfusion

journal_title

Transfusion

authors

Schreiber AB,Lambermont M,Strosberg AD,Wybran J

doi

10.1046/j.1537-2995.1981.21281178153.x

subject

Has Abstract

pub_date

1981-03-01 00:00:00

pages

178-83

issue

2

eissn

0041-1132

issn

1537-2995

journal_volume

21

pub_type

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