Mitomycin C test for diagnostic differentiation of idiopathic aplastic anemia and Fanconi anemia.

Abstract:

:A cytogenetic test was developed to enable the diagnostic differentiation between Fanconi anemia and the group of disorders classified as "idiopathic" aplastic anemia. The method is based on the effect of the bifunctional alkylating agent mitomycin C (MMC) on chromosomes of peripheral lymphocytes in culture 92 to 96 hours. At the highest concentration of 80 ng/ml of MMC, about a 50-fold increase of chromosomal breaks and a 200-fold increase of radial figures was observed in Fanconi anemia lymphocytes in comparison with the average values for aplastic anemia cells or cells of healthy control subjects. In addition, sister chromatoid exchange analysis of cells treated with MMC demonstrated a marked inhibition of mitotic activity of lymphocytes of patients with increased sensitivity to MMC. It is advocated that the MMC-stress test should be used in each case of pancytopenia, as part of the therapy planning. The cytoreduction regimens used prior to bone marrow transplantation may produce lethal complications in patients with high clastogenic response to MMC.

journal_name

Pediatrics

journal_title

Pediatrics

authors

Cervenka J,Arthur D,Yasis C

subject

Has Abstract

pub_date

1981-01-01 00:00:00

pages

119-27

issue

1

eissn

0031-4005

issn

1098-4275

journal_volume

67

pub_type

杂志文章
  • Surfactant replacement therapy for severe neonatal respiratory distress syndrome: an international randomized clinical trial. Collaborative European Multicenter Study Group.

    abstract::In a randomized multicenter trial, involving the collaboration of eight European neonatal intensive care units, the efficacy of replacement therapy with a new surfactant preparation (Curosurf) was tested in 146 patients with severe neonatal respiratory distress syndrome. Criteria for entry included birth weight 700 to...

    journal_title:Pediatrics

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:

    authors:

    更新日期:1988-11-01 00:00:00

  • Parental smoking and vascular damage in their 5-year-old children.

    abstract:BACKGROUND:The relation between smoke exposure in early life, the prenatal period in particular, and the vascular development of young children is largely unknown. METHODS:Data from the birth cohort participating in the WHISTLER-Cardio study were used to relate the smoking of parents during pregnancy to subsequent vas...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.2011-0249

    authors: Geerts CC,Bots ML,van der Ent CK,Grobbee DE,Uiterwaal CS

    更新日期:2012-01-01 00:00:00

  • First-week protein and energy intakes are associated with 18-month developmental outcomes in extremely low birth weight infants.

    abstract:OBJECTIVE:We sought to evaluate the association between early protein and energy intake and neurodevelopment and growth of extremely low birth weight (<1000 g) infants. STUDY DESIGN:Daily protein and energy intakes were collected by chart review for the first 4 weeks of life on 148 extremely low birth weight survivors...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.2008-0211

    authors: Stephens BE,Walden RV,Gargus RA,Tucker R,McKinley L,Mance M,Nye J,Vohr BR

    更新日期:2009-05-01 00:00:00

  • Maternal, neonatal, and placental features associated with diffuse chorioamniotic hemosiderosis, with special reference to neonatal morbidity and mortality.

    abstract:OBJECTIVE:Our purpose was to examine the significance of diffuse chorioamniotic hemosiderosis (DCH) on neonatal morbidity and mortality. METHODS:Using data from a retrospective case-control study, we analyzed 46 singleton placentas with DCH from infants who were delivered and/or admitted to the neonatal intensive care...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.113.4.800

    authors: Ohyama M,Itani Y,Yamanaka M,Goto A,Kato K,Ijiri R,Tanaka Y

    更新日期:2004-04-01 00:00:00

  • Carbohydrate absorption from fruit juice in young children.

    abstract:OBJECTIVE:To compare carbohydrate absorption following ingestion of apple juice and white grape juice in 28 healthy children. DESIGN:Randomized, double-blind crossover study. SETTING:Outpatient pediatric clinic at Maimonides Medical Center. PARTICIPANTS:A total of 18 healthy infants (mean age 6.3 months) and 10 todd...

    journal_title:Pediatrics

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Smith MM,Davis M,Chasalow FI,Lifshitz F

    更新日期:1995-03-01 00:00:00

  • The evaluation of sexual abuse in children.

    abstract::This clinical report serves to update the statement titled "Guidelines for the Evaluation of Sexual Abuse of Children," which was first published in 1991 and revised in 1999. The medical assessment of suspected sexual abuse is outlined with respect to obtaining a history, physical examination, and appropriate laborato...

    journal_title:Pediatrics

    pub_type: 杂志文章,实务指引

    doi:10.1542/peds.2005-1336

    authors: Kellogg N,American Academy of Pediatrics Committee on Child Abuse and Neglect.

    更新日期:2005-08-01 00:00:00

  • Hypothalamic-pituitary-adrenal axis suppression in asthmatic school children.

    abstract:BACKGROUND AND OBJECTIVE:Hypothalamic-pituitary-adrenal axis suppression (HPAS) when treating children with corticosteroids is thought to be rare. Our objective was to determine the prevalence of and predictive factors for various degrees of HPAS. METHODS:Clinical features of HPAS, doses, adherence, asthma score, and ...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.2012-1147

    authors: Zöllner EW,Lombard CJ,Galal U,Hough FS,Irusen EM,Weinberg E

    更新日期:2012-12-01 00:00:00

  • Use of visual training for reading disabilities: a review.

    abstract::Visual training continues to be used as a remediation technique for reading disabilities despite consistent evidence that it does not provide effective treatment. A review of the ophthalmologic, optometric, and psychological literature relating to the assumption that a relationship exists between reading failure and p...

    journal_title:Pediatrics

    pub_type: 杂志文章,评审

    doi:

    authors: Metzger RL,Werner DB

    更新日期:1984-06-01 00:00:00

  • Omega-3 Long-chain Polyunsaturated Fatty Acids for Bronchopulmonary Dysplasia: A Meta-analysis.

    abstract:CONTEXT:Previous studies have suggested that intervention with omega-3 long-chain polyunsaturated fatty acids (N-3 LCPUFAs), especially docosahexaenoic acid, can reduce the incidence of bronchopulmonary dysplasia (BPD) in preterm infants. However, conflicting results have been reported. OBJECTIVE:We conducted this met...

    journal_title:Pediatrics

    pub_type: 杂志文章,meta分析

    doi:10.1542/peds.2019-0181

    authors: Wang Q,Zhou B,Cui Q,Chen C

    更新日期:2019-07-01 00:00:00

  • Overcrowding crisis in our nation's emergency departments: is our safety net unraveling?

    abstract::Emergency departments (EDs) are a vital component in our health care safety net, available 24 hours a day, 7 days a week, for all who require care. There has been a steady increase in the volume and acuity of patient visits to EDs, now with well over 100 million Americans (30 million children) receiving emergency care...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.2004-1287

    authors: American Academy of Pediatrics Committee on Pediatric Emergency Medicine.

    更新日期:2004-09-01 00:00:00

  • Dietary fiber in weaning foods of young children.

    abstract:OBJECTIVE:An evaluation of the opportunity to introduce fiber in the diet of weaning infants. METHODS:A descriptive review of the literature to assess the role of fiber in the following dietary interventions: (1) definition of the nutrient amounts that meet the weaning infant's physiologic needs; (2) modulation of dig...

    journal_title:Pediatrics

    pub_type: 杂志文章,评审

    doi:

    authors: Agostoni C,Riva E,Giovannini M

    更新日期:1995-11-01 00:00:00

  • Sodium space and intravascular volume: dietary sodium effects in cystic fibrosis and healthy adolescent subjects.

    abstract:OBJECTIVE:To assess the physiologic response to salt depletion in subjects with cystic fibrosis (CF) and control male adolescents for sodium balance, sodium space, and stimulation of the renin-angiotensin-aldosterone axis. DESIGN:Seven subjects with CF and six controls received a salt-replete (150 or 290 mmol NaCl per...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.101.1.48

    authors: Legris GJ,Dearborn D,Stern RC,Geiss CL,Hopfer U,Douglas JG,Doershuk CF

    更新日期:1998-01-01 00:00:00

  • Considering complementary and alternative medicine alternatives in cases of life-threatening illness: applying the best-interests test.

    abstract::In this article we explore decision-making about treatment when a child faces a life-threatening illness but conventional treatment presents substantial risk and uncertain benefit. When is it acceptable for parents to decide to use complementary and alternative medicine as an alternative, rather than a complement, to ...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.2010-2720F

    authors: Gilmour J,Harrison C,Asadi L,Cohen MH,Aung S,Vohra S

    更新日期:2011-11-01 00:00:00

  • Effect of intramuscular vitamin E on mortality and intracranial hemorrhage in neonates of 1000 grams or less.

    abstract::A randomized, double-blind study to determine the effect of intramuscular vitamin E on mortality and intracranial hemorrhage (ICH) was performed. One hundred forty-nine neonates with birth weights less than or equal to 1000 g and less than or equal to 24 hours of age were grouped by weight (501 to 750 g and 751 to 100...

    journal_title:Pediatrics

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Fish WH,Cohen M,Franzek D,Williams JM,Lemons JA

    更新日期:1990-04-01 00:00:00

  • Infection prevention and control in pediatric ambulatory settings.

    abstract::Since the American Academy of Pediatrics published a statement titled "Infection Control in Physicians' Offices" (Pediatrics. 2000;105[6]:1361-1369), there have been significant changes that prompted this updated statement. Infection prevention and control is an integral part of pediatric practice in ambulatory medica...

    journal_title:Pediatrics

    pub_type: 杂志文章,实务指引

    doi:10.1542/peds.2007-1734

    authors: American Academy of Pediatrics Committee on Infectious Diseases.

    更新日期:2007-09-01 00:00:00

  • Developmental Outcomes of Preterm Infants With Neonatal Hypoglycemia.

    abstract:BACKGROUND AND OBJECTIVES:Neonatal hypoglycemia has been associated with abnormalities on brain imaging and a spectrum of developmental delays, although historical and recent studies show conflicting results. We compared the cognitive, academic, and behavioral outcomes of preterm infants with neonatal hypoglycemia with...

    journal_title:Pediatrics

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1542/peds.2016-1424

    authors: Goode RH,Rettiganti M,Li J,Lyle RE,Whiteside-Mansell L,Barrett KW,Casey PH

    更新日期:2016-12-01 00:00:00

  • Long-term follow-up study of serum immunoglobulin G and immunoglobulin A antibodies after Helicobacter pylori eradication.

    abstract:OBJECTIVE:There have been few studies concerning serum titers of anti-Helicobacter pylori immunoglobulin G (IgG) antibody >12 months after eradication of the original infection. Moreover, clinical usefulness of immunoglobulin A (IgA) antibody levels remains to be established. The purpose of this study was to investigat...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.104.2.e22

    authors: Kato S,Furuyama N,Ozawa K,Ohnuma K,Iinuma K

    更新日期:1999-08-01 00:00:00

  • Pacifying effects of nonnutritive sucking on term and preterm neonates during heelstick procedures.

    abstract::Behavioral state, heart rate, and respiration were monitored during heelstick procedures in samples of healthy, term neonates (N = 48) and preterm neonates treated in minimal care (N = 48) and intensive care (N = 48) nurseries. The treated infants who were given pacifiers spent significantly less time fussing and cryi...

    journal_title:Pediatrics

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Field T,Goldson E

    更新日期:1984-12-01 00:00:00

  • Chromosomal integration of human herpesvirus 6 is the major mode of congenital human herpesvirus 6 infection.

    abstract:OBJECTIVE:We examined the frequency and characteristics of chromosomally integrated human herpesvirus 6 among congenitally infected children. METHODS:Infants with and without congenital human herpesvirus 6 infection were prospectively monitored. Cord blood mononuclear cell, peripheral blood mononuclear cell, saliva, u...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.2007-2838

    authors: Hall CB,Caserta MT,Schnabel K,Shelley LM,Marino AS,Carnahan JA,Yoo C,Lofthus GK,McDermott MP

    更新日期:2008-09-01 00:00:00

  • Local anesthetic and stylet styles: factors associated with resident lumbar puncture success.

    abstract:OBJECTIVE:To assess the effects of procedural techniques, local anesthetic use, and postgraduate training level on lumbar puncture (LP) success rates. METHODS:In this prospective observational study, medical students and residents ("trainees") reported techniques used for infant LPs in an urban teaching emergency depa...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.2005-0519

    authors: Baxter AL,Fisher RG,Burke BL,Goldblatt SS,Isaacman DJ,Lawson ML

    更新日期:2006-03-01 00:00:00

  • A review of five years' experience with rubella vaccine in the United States.

    abstract::National morbidity figures show a decline in reported rubella and congenital rubella syndrome since 1969, concurrent with widespread use of rubella vaccine. In addition, no nationwide outbreak, such as the 1963-1964 epidemic, has occurred, though on the basis of long-term secular trends, one would be expected between ...

    journal_title:Pediatrics

    pub_type: 杂志文章,评审

    doi:

    authors: Modlin JF,Brandling-Bennett AD,Witte JJ,Campbell CC,Meyers JD

    更新日期:1975-01-01 00:00:00

  • Increased rates of morbidity, mortality, and charges for hospitalized children with public or no health insurance as compared with children with private insurance in Colorado and the United States.

    abstract:BACKGROUND:There has been a gradual decrease in the proportion of children covered by private health insurance in Colorado and the United States with a commensurate increase in those with public insurance or having no insurance which may impact access to care and outcomes. OBJECTIVE:The purpose of this work was to det...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.2006-0162

    authors: Todd J,Armon C,Griggs A,Poole S,Berman S

    更新日期:2006-08-01 00:00:00

  • Chemical-biological terrorism and its impact on children: a subject review. American Academy of Pediatrics. Committee on Environmental Health and Committee on Infectious Diseases.

    abstract::There is an increasing threat that chemical and biological weapons will be used on a civilian population in an act of domestic terrorism. Casualties among adults and children could be significant in such an event. Federal, state, and local authorities have begun extensive planning to meet a chemical-biological inciden...

    journal_title:Pediatrics

    pub_type: 杂志文章,评审

    doi:10.1542/peds.105.3.662

    authors:

    更新日期:2000-03-01 00:00:00

  • Revised guidelines for prevention of early-onset group B streptococcal (GBS) infection. American Academy of Pediatrics Committee on Infectious Diseases and Committee on Fetus and Newborn.

    abstract::In 1992, the Committee on Infectious Diseases and Committee on Fetus and Newborn of the American Academy of Pediatrics provided guidelines for prevention of early-onset group B streptococcal (GBS) disease through intrapartum chemoprophylaxis of selected maternal GBS carriers. The guidelines were based on demonstrated ...

    journal_title:Pediatrics

    pub_type: 指南,杂志文章,实务指引

    doi:10.1542/peds.99.3.489

    authors:

    更新日期:1997-03-01 00:00:00

  • The best-interest standard is not applied for neonatal resuscitation decisions.

    abstract:OBJECTIVE:Legal and ethical standards require resuscitation when it is considered to be in the patient's best interest. We hypothesized that newborn infants might be dealt with according to different standards, compared with older patients. METHODS:An anonymous questionnaire describing 8 currently incompetent patients...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.2007-1520

    authors: Janvier A,Leblanc I,Barrington KJ

    更新日期:2008-05-01 00:00:00

  • Oxygen Saturation Targets in Preterm Infants and Outcomes at 18-24 Months: A Systematic Review.

    abstract:CONTEXT:The optimal oxygen saturation target for extremely preterm infants remains unclear. OBJECTIVE:To systematically review evidence evaluating the effect of lower (85%-89%) versus higher (91%-95%) pulse oxygen saturation (Spo2) target on mortality and neurodevelopmental impairment (NDI) at 18 to 24 months. DATA S...

    journal_title:Pediatrics

    pub_type: 杂志文章,评审

    doi:10.1542/peds.2016-1609

    authors: Manja V,Saugstad OD,Lakshminrusimha S

    更新日期:2017-01-01 00:00:00

  • High-deductible health plans.

    abstract::High-deductible health plans (HDHPs) are insurance policies with higher deductibles than conventional plans. The Medicare Prescription Drug Improvement and Modernization Act of 2003 linked many HDHPs with tax-advantaged spending accounts. The 2010 Patient Protection and Affordable Care Act continues to provide for HDH...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.2014-0555

    authors: Committee on Child Health Financing.

    更新日期:2014-05-01 00:00:00

  • Recurrent infections, hypotonia, and mental retardation caused by duplication of MECP2 and adjacent region in Xq28.

    abstract:OBJECTIVE:Our goal was to describe the neurologic and clinical features of affected males from families with X-linked patterns of severe mental retardation, hypotonia, recurrent respiratory infection, and microduplication of Xq28 that consistently includes the MECP2 (methyl-CpG binding protein 2) gene. STUDY DESIGN:To...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.2006-0395

    authors: Friez MJ,Jones JR,Clarkson K,Lubs H,Abuelo D,Bier JA,Pai S,Simensen R,Williams C,Giampietro PF,Schwartz CE,Stevenson RE

    更新日期:2006-12-01 00:00:00

  • Identification of children at risk for lead poisoning: an evaluation of routine pediatric blood lead screening in an HMO-insured population.

    abstract:OBJECTIVES:To estimate the prevalence of elevated blood lead levels in children receiving well-care checkups; and to evaluate the effectiveness of certain key risk factors in detecting children at higher risk for elevated blood lead levels. DESIGN:Cross-sectional study. SETTING:Two facilities of the Kaiser Permanente...

    journal_title:Pediatrics

    pub_type: 杂志文章,多中心研究

    doi:

    authors: Haan MN,Gerson M,Zishka BA

    更新日期:1996-01-01 00:00:00

  • Pepsin, a marker of gastric contents, is increased in tracheal aspirates from preterm infants who develop bronchopulmonary dysplasia.

    abstract:OBJECTIVE:The objective of this study was to study the association between pepsin in tracheal aspirate samples and the development of bronchopulmonary dysplasia in preterm infants. METHODS:Serial tracheal aspirate samples were collected during the first 28 days from mechanically ventilated preterm neonates. Bronchopul...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.2007-0056

    authors: Farhath S,He Z,Nakhla T,Saslow J,Soundar S,Camacho J,Stahl G,Shaffer S,Mehta DI,Aghai ZH

    更新日期:2008-02-01 00:00:00