Abstract：
BACKGROUND:Isoflurane has been reported to induce caspase-3 activation, which may induce neurotoxicity and contribute to the pathogenesis of Alzheimer's disease. However, the underlying mechanism is largely unknown, especially whether or not isoflurane can induce ryanodine receptors (RyRs)-associated endoplasmic reticulum (ER) stress, leading to caspase-3 activation. We therefore assessed the effects of isoflurane on RyRs-associated ER stress. METHODS:We treated primary neurones from wild-type (C57BL/6J) mice with 1% and 2% isoflurane for 1, 3, or 6 h. We then measured levels of C/EBP homologous protein (CHOP) and caspase-12, two ER stress markers, using immunocytochemistry staining and western blotting analysis. Dantrolene (5 μM), the antagonist of RyRs, was used to investigate the role of RyRs in the isoflurane-induced ER stress and caspase-3 activation. RESULTS:Isoflurane 2% for 6 h treatment increased the levels of CHOP (876% vs 100%, P=0.00009) and caspase-12 (276% vs 100%, P=0.006), and induced caspase-3 activation in the neurones. The administration of 2% isoflurane for 3 h (shorter duration), however, only increased the levels of CHOP (309% vs 100%, P=0.003) and caspase-12 (266% vs 100%, P=0.001), without causing caspase-3 activation. The isoflurane-induced ER stress (CHOP: F=16.64, P=0.0022; caspase-12: F=6.13, P=0.0383) and caspase-3 activation (F=32.06, P=0.0005) were attenuated by the dantrolene treatment. CONCLUSIONS:These data imply that isoflurane might induce caspase-3 activation by causing ER stress through RyRs, and dantrolene could attenuate the isoflurane-induced ER stress and caspase-3 activation. Further investigations of the potential neurotoxicity of isoflurane are needed.
journal_name
Br J Anaesthjournal_title
British journal of anaesthesiaauthors
Wang H,Dong Y,Zhang J,Xu Z,Wang G,Swain CA,Zhang Y,Xie Zdoi
10.1093/bja/aeu053subject
Has Abstractpub_date
2014-10-01 00:00:00pages
695-707issue
4eissn
0007-0912issn
1471-6771pii
S0007-0912(17)30762-6journal_volume
113pub_type
杂志文章abstract：:The induction dose requirements of propofol were compared in three age groups in 300 unpremedicated healthy Chinese children: group A, younger than 2 yr (n = 48); group B, 2-5 yr (n = 117); group C, 6-12 yr (n = 135). Patients in each group were allocated randomly to receive one of eight doses of propofol (1.2, 1.4, 1...
journal_title：British journal of anaesthesia
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1093/bja/68.1.64
更新日期：1992-01-01 00:00:00
abstract：BACKGROUND:Double-lumen tubes (DLTs) are frequently used to establish one-lung ventilation (OLV). Their correct placement is crucial. We hypothesized that electrical impedance tomography (EIT) reliably displays distribution of ventilation between left and right lung and may thus be used to verify correct DLT placement ...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1093/bja/aen166
更新日期：2008-09-01 00:00:00
abstract：BACKGROUND:Dexmedetomidine (DEX) has been shown to provide good perioperative haemodynamic stability with decreased intraoperative opioid requirements. It may have neural protective effects, and thus may be a suitable anaesthetic adjuvant to neurosurgical anaesthesia. METHODS:Fifty-four patients scheduled for elective...
journal_title：British journal of anaesthesia
pub_type： 杂志文章,随机对照试验
doi：10.1093/bja/ael220
更新日期：2006-11-01 00:00:00
abstract：:The study of rare families with inherited pain insensitivity can identify new human-validated analgesic drug targets. Here, a 66-yr-old female presented with nil requirement for postoperative analgesia after a normally painful orthopaedic hand surgery (trapeziectomy). Further investigations revealed a lifelong history...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1016/j.bja.2019.02.019
更新日期：2019-08-01 00:00:00
abstract：:We have compared the effects of dexmedetomidine and propofol on endocrine, metabolic, inflammatory and cardiovascular responses in patients in the intensive care unit (ICU) after major surgery. Twenty patients who were expected to require 8 h of post-operative sedation and ventilation were allocated randomly to receiv...
journal_title：British journal of anaesthesia
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1093/bja/86.5.650
更新日期：2001-05-01 00:00:00
abstract：BACKGROUND:Blood flow patterns are important modifiers of platelet interactions with plasma coagulation factors. However, it is not feasible to evaluate rheological effects of haemodilution on coagulation using conventional coagulation testing. METHODS:We evaluated thrombus formation with a microchip-based flow-chambe...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1093/bja/aet229
更新日期：2013-12-01 00:00:00
abstract：:Forty patients who had undergone coronary artery graft surgery and who required vasodilator therapy for postoperative hypertension were given infusions of either alfentanil or morphine together with bolus doses of midazolam for sedation and analgesia while ventilation was controlled artificially. Sodium nitroprusside ...
journal_title：British journal of anaesthesia
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1093/bja/61.6.669
更新日期：1988-12-01 00:00:00
abstract：BACKGROUND:The objective of this study was to determine whether assessment of stroke volume (SV) and measurement of exhaled end-tidal carbon dioxide [Formula: see text] during an end-expiratory occlusion (EEO) test can predict fluid responsiveness in the operating theatre. METHODS:Forty-two subjects monitored by oesop...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1093/bja/aet582
更新日期：2014-06-01 00:00:00
abstract：:Conventional extradural analgesia during labour with 0.25-0.375% bupivacaine may induce motor weakness and subjective sensory deficit, reducing maternal satisfaction. Even in a regimen for ambulatory extradural analgesia (0.1% bupivacaine-fentanyl 2 micrograms ml-1), a potential for proprioreception impairment exists,...
journal_title：British journal of anaesthesia
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1093/bja/76.2.319
更新日期：1996-02-01 00:00:00
abstract：:The effect of the administration of propranolol 0.15 mg per kg body weight i.v. on the metabolic response to pelvic surgery was compared with results in similar patients who had not received propranolol. The only significant difference between the two groups was a lower plasma insulin concentration in the beta-blockad...
journal_title：British journal of anaesthesia
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1093/bja/52.12.1231
更新日期：1980-12-01 00:00:00
abstract：:We studied the size 4 laryngeal mask airway (LMA) to test the hypothesis that oropharyngeal leak pressure and fibreoptic position improves with increasing cuff volume. After LMA insertion, 50 anaesthetized adult patients had the cuff inflated in 5-ml increments to 40 ml. Oropharyngeal leak pressure was optimal at 15 m...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1093/bja/81.2.186
更新日期：1998-08-01 00:00:00
abstract：BACKGROUND:There has been a renewed interest in nitric oxide donor drugs, such as nitroglycerin, delivered by the inhalational route for treatment of pulmonary arterial hypertension (PAH). We investigated the acute effects of inhaled nitroglycerin on pulmonary and systemic haemodynamics in children with PAH associated ...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1093/bja/ael112
更新日期：2006-08-01 00:00:00
abstract：BACKGROUND:The efficacy of opioids typically decreases after long-term use owing to the development of tolerance. Glial activation and the upregulation of proinflammatory cytokines are related to the induction of tolerance. We investigated the effect of leukemia inhibitory factor (LIF) on morphine analgesia and toleran...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1093/bja/aew247
更新日期：2016-10-01 00:00:00
abstract：BACKGROUND:Increasing fresh gas flow (FGF) to a circle breathing system reduces carbon dioxide (CO2) absorbent consumption. We assessed the environmental and economic impacts of this trade-off between gas flow and absorbent consumption when no inhalational anaesthetic agent is used. METHODS:A test lung with fixed CO2 ...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1016/j.bja.2020.07.043
更新日期：2020-11-01 00:00:00
abstract：:A recently installed anti-pollution system, initially designed to function in a passive mode, was evaluated, but found to be inconstant in its performance. A simple method of making the system active is described and evaluated. ...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1093/bja/49.5.439
更新日期：1977-05-01 00:00:00
abstract：BACKGROUND:Pain management in craniotomy patients is challenging, with mild-to-moderate pain intensity, moderate-to-high risk of postoperative nausea and vomiting (PONV), and potentially catastrophic consequences of analgesic-related side-effects. The aim of this study was to determine whether i.v. parecoxib administer...
journal_title：British journal of anaesthesia
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1093/bja/aer223
更新日期：2011-09-01 00:00:00
abstract：BACKGROUND:Circadian rhythms coordinate almost all physiological functions and are implicated in major disease development. Even though circadian rhythms have a major impact on human health, little is known about how they affect general anaesthesia. The purpose of this study was to understand if the time of day affects...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1016/j.bja.2018.06.027
更新日期：2018-10-01 00:00:00
abstract：:Effects of ageing were studied on the neural blockade and the pharmacokinetics after subarachnoid administration of 0.5% bupivacaine 3 ml in 8% glucose in two groups of 15 male patients, between 20 and 55 yr and older than 55 yr, respectively. The blockade was performed with the patient in the sitting position. Two mi...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1093/bja/60.2.187
更新日期：1988-02-01 00:00:00
abstract：BACKGROUND:Intraperitoneal insufflation of carbon dioxide (CO2) may promote collapse of dependent lung regions. The present study was undertaken to study the effects of CO2-pneumoperitoneum (CO2-PP) on atelectasis formation, arterial oxygenation, and arterial to end-tidal PCO2-gradient (Pa-E'(CO2)). METHODS:Fifteen an...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1093/bja/aep102
更新日期：2009-08-01 00:00:00
abstract：:Recommendations for resuscitation of patients in early haemorrhagic shock, with active ongoing bleeding, have evolved in recent years. This review covers current theories of the pathophysiology of shock and recommended treatments, including damage control surgery, deliberate hypotensive management, administration of a...
journal_title：British journal of anaesthesia
pub_type： 杂志文章,评审
doi：10.1093/bja/aes389
更新日期：2012-12-01 00:00:00
abstract：:We studied 133 women given a combined spinal-epidural for analgesia in labour. The initial intrathecal dose contained bupivacaine 2.5 mg with fentanyl 25 micrograms. When the mothers were comfortable, they were allocated randomly to one of three groups: continuous infusion (group Cl, n = 46), midwife top-ups (group MW...
journal_title：British journal of anaesthesia
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1093/bja/82.2.233
更新日期：1999-02-01 00:00:00
abstract：BACKGROUND:Ultrasound-guided internal jugular venous access increases the rate of successful cannulation and reduces the incidence of complications, compared with the landmark technique. Three transducer orientation approaches have been proposed for this procedure: short-axis (SAX), long-axis (LAX) and oblique-axis (OA...
journal_title：British journal of anaesthesia
pub_type： 杂志文章,随机对照试验
doi：10.1093/bja/aev399
更新日期：2016-03-01 00:00:00
abstract：BACKGROUND:Anaesthetic drugs act at sites within the brain that undergo profound changes during typical ageing. We postulated that anaesthesia-induced brain dynamics observed in the EEG change with age. METHODS:We analysed the EEG in 155 patients aged 18-90 yr who received propofol (n=60) or sevoflurane (n=95) as the ...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1093/bja/aev213
更新日期：2015-07-01 00:00:00
abstract：:We have studied the efficacy of preoxygenation using the Triservice Anaesthetic Apparatus (TSA) in healthy patients and found it to be unsatisfactory. An alternative technique suitable for field use was explored. The Mapleson D system (Bain) was found to be suitable for preoxygenation with a fresh gas flow of only 4 l...
journal_title：British journal of anaesthesia
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1093/bja/66.2.196
更新日期：1991-02-01 00:00:00
abstract：:In 1849 John Snow, already the leading anaesthetic practitioner and innovator of his day, made a historic contribution to the epidemiology of infectious disease by his famous study of the distribution of cholera around the area of Broad Street in London. We report on our experience as anaesthetists in a field hospital...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1093/bja/75.6.810
更新日期：1995-12-01 00:00:00
abstract：:The haemodynamic responses to isovolumic anaemia (dextran-for-blood exchange) were studied in seven dogs pretreated with propranolol 20 mg/kg body weight for 3 weeks. Adrenergic beta-receptor blockade did not prevent significant increases in heart rate (up to 19%). Cardiac output increased by about 20% while systemic ...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：BACKGROUND:Intubation of a patient with limited cervical spine movement or in whom movement of the cervical spine is not desirable is always a challenge even to the most experienced anaesthesiologist. The intubating laryngeal mask airway (ILMA) and the Bullard laryngoscope (BL) are two instruments recommended for endot...
journal_title：British journal of anaesthesia
pub_type： 杂志文章,随机对照试验
doi：10.1093/bja/aem127
更新日期：2007-08-01 00:00:00
abstract：BACKGROUND:Fresh-frozen plasma (FFP) is widely used in critically ill patients, despite a weak evidence base. Factors that influence the decision to transfuse FFP before intravascular catheter insertion are poorly described. METHODS:We undertook a case-controlled study based on a prospective cohort study of 1923 admis...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1093/bja/aes337
更新日期：2012-12-01 00:00:00
abstract：:We have investigated whether nitrous oxide antagonizes or augments the CNS stimulant action of laudanosine in mice by comparing the mean convulsive doses (CD50 (SE] of a control group and those following pretreatment with 65% nitrous oxide in oxygen for 20 and 180 min. Nitrous oxide significantly increased CD50 from 4...
journal_title：British journal of anaesthesia
pub_type： 杂志文章
doi：10.1093/bja/65.5.704
更新日期：1990-11-01 00:00:00
abstract：:Dose-response relationships for the antagonism of intermediate-acting neuromuscular blocking agents have not been evaluated previously in children. We have examined the dose-response relationships for neostigmine antagonism of 90% rocuronium-induced neuromuscular block in children and adults, during nitrous oxide-1 MA...
journal_title：British journal of anaesthesia
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1093/bja/77.6.710
更新日期：1996-12-01 00:00:00
