Abstract：
:Lymphocytes isolated from Lewis rats immunised with protein antigen in adjuvant were stimulated to proliferate in vitro by splenic dendritic cells (DC) which had been pulsed with purified homologous myelin basic protein (MBP). By contrast, in parallel experiments, lymphocytes did not respond to ovalbumin unless the protein was first processed by macrophages by a chloroquine-sensitive mechanism. DC, pulsed with rat MBP at concentrations as low as 6 micrograms/ml, activated lymphocytes for transfer of severe experimental autoimmune encephalomyelitis (EAE). MBP dissociating from myelin membranes in physiological medium, as well as MBP purified from highly acidic extracts of myelin, was effective for pulsing DC; preincubating myelin with macrophages led to a reduction rather than an enhancement in the severity of the EAE transferred. It is concluded that macrophage-mediated antigen processing is not required for immunogenic presentation of the determinants of MBP which cause EAE in Lewis rats. Furthermore, MBP-pulsed DC may prove useful in experiments requiring activation of encephalitogenic T cells.
journal_name
J Neuroimmunoljournal_title
Journal of neuroimmunologyauthors
Gautam AM,Glynn Pdoi
10.1016/0165-5728(89)90041-6subject
Has Abstractpub_date
1989-04-01 00:00:00pages
113-21issue
2eissn
0165-5728issn
1872-8421pii
0165-5728(89)90041-6journal_volume
22pub_type
杂志文章abstract：:After an injury to the central nervous system (CNS), activated microglia have been shown to contribute to the ongoing destructive processes leading to secondary neuronal degeneration. They can, however, also express neuroprotective activity. Studies from our laboratory point to the existence of a physiological T cell-...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2003.10.049
更新日期：2004-01-01 00:00:00
abstract：:Active immunization with myelin basic protein (MBP) induces experimental allergic encephalomyelitis (EAE) in a variety of animal species, including rats and mice. We have previously described the ability of the newly described mouse lambda (lambda) variable (V) region V lambda x, to confer MBP reactivity to an Ab. In ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(96)00072-0
更新日期：1996-10-01 00:00:00
abstract：:Multiple sclerosis (MS) is an incurable inflammatory demyelinating disease. We investigated one calcitriol dose plus vitamin D3 (calcitriol/+D) as a demyelinating disease treatment in experimental autoimmune encephalomyelitis (EAE). Evidence that calcitriol-vitamin D receptor pathway deficits may promote MS, and data ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2013.07.016
更新日期：2013-10-15 00:00:00
abstract：:We have analyzed a CA repeat polymorphism localized 46-kb upstream of the Fas ligand gene in Spanish and American populations that include 139 healthy controls and a cohort of 177 unrelated relapsing and remitting multiple sclerosis (MS) patients. The MS patients consisted of two groups, one with a family history of M...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(01)00309-5
更新日期：2001-06-01 00:00:00
abstract：:A large body of immunologic, epidemiologic, and genetic data indicate that tissue injury in multiple sclerosis (MS) results from an abnormal immune response to one or more myelin antigens that develops in genetically susceptible individuals after exposure to an as-yet undefined causal agent. The genetic component of M...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章,评审
doi：10.1016/s0165-5728(00)00444-6
更新日期：2001-02-15 00:00:00
abstract：:Intercellular adhesion molecule-1 (ICAM-1) is a cell surface glycoprotein which can be induced on astrocytes, the major glial cell of the central nervous system (CNS). In this study, we examined the effect of three proinflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(94)90083-3
更新日期：1994-05-01 00:00:00
abstract：:Autotaxin (ATX) is an enzyme producing lysophosphatidic acid (LPA) from lysophosphatidyl choline (LPC) and it is up-regulated in inflammatory conditions such as various cancers, arthritis and multiple sclerosis (MS). Numerous studies have shown that the LPA signaling gives rise to angiogenesis, mitosis, cell prolifera...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2014.06.006
更新日期：2014-08-15 00:00:00
abstract：:Among the death ligands of the tumor necrosis factor/nerve growth factor (TNF/NGF) superfamily, TNF-related apoptosis-inducing ligand (TRAIL) is considered to play a unique role due to its binding to both apoptosis-inducing and -blocking membranous receptors, apoptosis-independent effects and distinct species differen...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(01)00450-7
更新日期：2002-01-01 00:00:00
abstract：:We evaluated the concentration of antineuronal antibodies in paired cerebrospinal fluid (CSF) and serum samples from 19 patients with central and peripheral paraneoplastic neurological syndromes (PNS), using an enzyme linked immunosorbent assay (ELISA) employing recombinant antineuronal antigens (HuD, Yo, Ri, CV2/CRMP...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2006.11.008
更新日期：2007-02-01 00:00:00
abstract：:The effects of acute or chronic in vivo or in vitro exposures to supra-physiologic doses of isoproterenol, insulin or dexamethasone on rat splenic lymphocyte proliferation were investigated. Acutely, all in vivo challenges inhibited mitogen-induced lymphocyte proliferation, and correlated to increased corticosterone l...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(87)90079-8
更新日期：1987-10-01 00:00:00
abstract：:The in vivo relationship of interleukin-2 (IL-2) to the local humoral immune response within the central nervous system (CNS) in patients with multiple sclerosis (MS) is hitherto largely unknown. Intrathecal levels of IL-2 and soluble IL-2 receptors (sIL-2R) were correlated to the local CNS synthesis of immunoglobulin...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(91)90070-n
更新日期：1991-04-01 00:00:00
abstract：:HIV-associated cognitive neurological disorders (HAND) prevail in the antiretroviral therapy era. Proteomics analysis of CSF revealed expression of Cu/Zn superoxide dismutase (Cu/Zn SOD) in Hispanic women with cognitive impairment (CI). We tested the hypothesis that there is reduced capacity of antioxidant enzymes in ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2008.10.013
更新日期：2009-01-03 00:00:00
abstract：:Longitudinal brain atrophy quantification is a critical efficacy measurement in multiple sclerosis (MS) clinical trials and the determination of No Evidence of Disease Activity (NEDA). Utilising fingolimod as a clinically validated therapy we evaluated the use of repeated brain tissue volume measures during chronic ex...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2018.02.016
更新日期：2018-05-15 00:00:00
abstract：:In the present work we investigated on rat splenocytes long-term interactions between opioid and cannabinoid drugs in terms of a common regulation of cAMP intracellular pathway. Both morphine and the synthetic cannabinoid compound CP-55,940 inhibited in a concentration-dependent manner the intracellular cAMP level in ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2003.09.006
更新日期：2003-12-01 00:00:00
abstract：:beta-Endorphin 1-31 and several structurally related peptides were tested for their ability to alter mitogen-induced T cell proliferation. Rat beta-endorphin 1-31 and human beta-endorphin 1-27 increased phytohemagglutinin (PHA)-induced [3H]thymidine incorporation into rat lymph node cells. However, when PHA-induced pr...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(90)90167-l
更新日期：1990-09-01 00:00:00
abstract：:Rat thymocytes incubated in RPMI medium for 120-300 min release a soluble factor of molecular weight below 10,000 Da which inhibits the binding of 3H-labeled muscarinic antagonists in an uncompetitive manner, i.e. it reduces maximal specific binding (Bmax) without changing the affinity of the ligand (KD). This factor ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(88)90119-1
更新日期：1988-03-01 00:00:00
abstract：:The B7/CD28:CTLA-4 costimulatory pathway plays a critical role in determining the fate of immune responses (activation vs. down-regulation) and is a highly promising therapeutic target for treating autoimmune diseases. In this review, we highlight the mechanisms by which this costimulatory pathway operates emphasizing...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章,评审
doi：10.1016/s0165-5728(98)00058-7
更新日期：1998-08-14 00:00:00
abstract：:Th17 cells play an important role in multiple sclerosis (MS) and its autoimmune model, experimental autoimmune encephalomyelitis (EAE). However, studies have not addressed how enhanced Th17 immune responses can affect demyelinating diseases. We induced EAE with MOG in RORγt transgenic C57BL/6 mice that overexpress a T...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2014.09.006
更新日期：2014-11-15 00:00:00
abstract：:Gangliosides modulate various T cell effector functions through poorly defined mechanisms. To begin to understand one of their effects, the present study examined how normal brain-derived gangliosides suppress T cell proliferation using the murine T cell line, EL4, as a model. Gangliosides inhibited EL4 cell growth by...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(98)00038-1
更新日期：1998-07-01 00:00:00
abstract：:We reviewed the records of 391 patients who had presented with a Clinically Isolated Syndrome and selected 205 who had performed a baseline spinal tap and MRI scan. We studied cerebrospinal fluid (CSF) and serum IgM oligoclonal bands (IgMOB) using agarose gel isoelectric focusing and analyzed the impact of baseline cl...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2013.01.011
更新日期：2013-04-15 00:00:00
abstract：:Predisposing factors before the onset of neuromyelitis optica spectrum disorders (NMOSD) have not been systematically evaluated by now. We investigated the detailed pre-onset history in consecutive NMOSD patients. Thirteen of the enrolled 53 NMOSD patients (24.5%) had accompanying autoimmune diseases, such as Sjögren'...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2020.577236
更新日期：2020-06-15 00:00:00
abstract：:The present investigations sought to determine whether the ventral tegmental area (VTA), basolateral amygdala (BLA), and nucleus accumbens shell (NAC) comprise a circuitry that mediates heroin-induced conditioned immunomodulation. Rats were given conditioning trials in which they received an injection of heroin upon p...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2011.06.002
更新日期：2011-08-15 00:00:00
abstract：:To elucidate the possible involvement of the inducible nitric oxide synthase (iNOS) and NO in the development of lymphocytic choriomeningitis (LCM), the consequences of inhibition of iNOS by the inhibitor aminoguanidine was examined in mice following intracerebral infection with LCM virus (LCMV). Aminoguanidine admini...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(96)00129-4
更新日期：1996-12-01 00:00:00
abstract：:We investigated T cell immunity to Epstein-Barr virus (EBV) in multiple sclerosis (MS) and assessed cross-reactivity of the anti-EBV cellular response with brain antigens. We measured the proliferation, cytotoxicity, and number of interferon-γ secreting cells following stimulation with autologous EBV-infected lymphobl...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2010.08.009
更新日期：2010-12-15 00:00:00
abstract：:Bacterial meningitis is - despite therapeutical progress during the last decades - still characterized by high mortality and severe permanent neurogical sequelae. The brain is protected from penetrating pathogens by both the blood-brain barrier and the innate immune system. Invading pathogens are recognized by so-call...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2016.09.003
更新日期：2016-10-15 00:00:00
abstract：:Elevated titers of serum antibodies against GM(1)-ganglioside are associated with a variety of autoimmune neuropathies. Although much evidence indicates that these autoantibodies play a primary role in the disease processes, the mechanism of their appearance is unclear. Low-affinity anti-GM(1) antibodies of the IgM is...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(01)00355-1
更新日期：2001-09-03 00:00:00
abstract：:To determine whether TNF and TRAIL death receptors (DR), and decoy receptors (DcR), play a role in oligodendrocyte depletion in the lesions of chronic multiple sclerosis (MS), we investigated the presence and functionality of these molecules on oligodendrocytes in MS and non-MS brain tissue and on human oligodendrocyt...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2007.05.018
更新日期：2007-08-01 00:00:00
abstract：:Following hypoglossal nerve transection in adult rats, immunoreactivity for complement factor C3 and one of its degradation products C3d as well as C4d and immunoglobulin G (IgG) was observed in the ipsilateral hypoglossal nucleus. Double-labelling experiments indicated that these antigens were present in perineuronal...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(92)90217-9
更新日期：1992-09-01 00:00:00
abstract：:Neuromyelitis optica (NMO) is a rare demyelinating disease, affecting selectively the optic nerve and the spinal cord. It was previously considered to be a severe variant of multiple sclerosis (MS) due to the similar pathological features and its resemblance to optico-spinal, or Japanese, MS, typical of Asian populati...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章,评审
doi：10.1016/j.jneuroim.2010.10.012
更新日期：2011-02-01 00:00:00
abstract：:Sprague-Dawley rats were injected intraperitoneally with a suspension of Trypanosoma brucei brucei. An early induction of major histocompatibility complex (MHC) class I antigens as well as an infiltration of macrophage-like cells and cytotoxic T-cells was detected with immunohistochemical techniques in circumventricul...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(89)90104-5
更新日期：1989-09-01 00:00:00
