Upregulation of HIF-1α via activation of ERK and PI3K pathway mediated protective response to microwave-induced mitochondrial injury in neuron-like cells.


:Microwave-induced learning and memory deficits in animal models have been gaining attention in recent years, largely because of increasing public concerns on growing environmental influences. The data from our group and others have showed that the injury of mitochondria, the major source of cellular adenosine triphosphate (ATP) in primary neurons, could be detected in the neuron cells of microwave-exposed rats. In this study, we provided some insights into the cellular and molecular mechanisms behind mitochondrial injury in PC12 cell-derived neuron-like cells. PC12 cell-derived neuron-like cells were exposed to 30 mW/cm(2) microwave for 5 min, and damages of mitochondrial ultrastructure could be observed by using transmission electron microscopy. Impairments of mitochondrial function, indicated by decrease of ATP content, reduction of succinate dehydrogenase (SDH) and cytochrome c oxidase (COX) activities, decrease of mitochondrial membrane potential (MMP), and increase of reactive oxygen species (ROS) production, could be detected. We also found that hypoxia-inducible factor-1 (HIF-1α), a key regulator responsible for hypoxic response of the mammalian cells, was upregulated in microwave-exposed neuron-like cells. Furthermore, HIF-1α overexpression protected mitochondria from injury by increasing the ATP contents and MMP, while HIF-1α silence promoted microwave-induced mitochondrial damage. Finally, we demonstrated that both ERK and PI3K signaling activation are required in microwave-induced HIF-1α activation and protective response. In conclusion, we elucidated a regulatory connection between impairments of mitochondrial function and HIF-1α activation in microwave-exposed neuron-like cells. By modulating mitochondrial function and protecting neuron-like cells against microwave-induced mitochondrial injury, HIF-1α represents a promising therapeutic target for microwave radiation injury.


Mol Neurobiol


Molecular neurobiology


Zhao L,Yang YF,Gao YB,Wang SM,Wang LF,Zuo HY,Dong J,Xu XP,Su ZT,Zhou HM,Zhu LL,Peng RY




Has Abstract


2014-12-01 00:00:00












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    abstract::Docosahexaenoic acid (DHA) is enriched in membrane phospholipids of the central nervous system (CNS) and has a role in aging and neuropsychiatric disorders. DHA is metabolized by the enzyme Alox15 to 17S-hydroxy-DHA, which is then converted to 7S-hydroperoxy,17S-hydroxy-DHA by a 5-lipoxygenase, and thence via epoxy in...

    journal_title:Molecular neurobiology

    pub_type: 杂志文章


    authors: Shalini SM,Ho CF,Ng YK,Tong JX,Ong ES,Herr DR,Dawe GS,Ong WY

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  • Deficiency of Tenascin-C Alleviates Neuronal Apoptosis and Neuroinflammation After Experimental Subarachnoid Hemorrhage in Mice.

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    journal_title:Molecular neurobiology

    pub_type: 杂志文章


    authors: Liu L,Fujimoto M,Nakano F,Nishikawa H,Okada T,Kawakita F,Imanaka-Yoshida K,Yoshida T,Suzuki H

    更新日期:2018-11-01 00:00:00

  • Calpain-Dependent ErbB4 Cleavage Is Involved in Brain Ischemia-Induced Neuronal Death.

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    journal_title:Molecular neurobiology

    pub_type: 杂志文章


    authors: Lu YM,Gao YP,Tao RR,Liao MH,Huang JY,Wu G,Han F,Li XM

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    journal_title:Molecular neurobiology

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    authors: Matthys A,Haegeman G,Van Craenenbroeck K,Vanhoenacker P

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    journal_title:Molecular neurobiology

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    authors: Zhang XS,Lu Y,Tao T,Wang H,Liu GJ,Liu XZ,Liu C,Xia DY,Hang CH,Li W

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    journal_title:Molecular neurobiology

    pub_type: 杂志文章


    authors: Li Z,Jagadapillai R,Gozal E,Barnes G

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    authors: Portela LV,Brochier AW,Haas CB,de Carvalho AK,Gnoato JA,Zimmer ER,Kalinine E,Pellerin L,Muller AP

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  • Maternal Separation-Induced Histone Acetylation Correlates with BDNF-Programmed Synaptic Changes in an Animal Model of PTSD with Sex Differences.

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    authors: Sun H,Zhang X,Kong Y,Gou L,Lian B,Wang Y,Jiang L,Li Q,Sun H,Sun L

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  • BAX-Depleted Retinal Ganglion Cells Survive and Become Quiescent Following Optic Nerve Damage.

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    pub_type: 杂志文章


    authors: Donahue RJ,Maes ME,Grosser JA,Nickells RW

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    authors: Gasparini S,Del Vecchio G,Gioiosa S,Flati T,Castrignano T,Legnini I,Licursi V,Ricceri L,Scattoni ML,Rinaldi A,Presutti C,Mannironi C

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    journal_title:Molecular neurobiology

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    更新日期:1998-02-01 00:00:00

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    journal_title:Molecular neurobiology

    pub_type: 杂志文章


    authors: Schönhofen P,de Medeiros LM,Bristot IJ,Lopes FM,De Bastiani MA,Kapczinski F,Crippa JA,Castro MA,Parsons RB,Klamt F

    更新日期:2015-08-01 00:00:00

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    authors: Henn L,Zanta NC,Girardi CEN,Suchecki D

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    journal_title:Molecular neurobiology

    pub_type: 杂志文章,评审


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    更新日期:1996-06-01 00:00:00

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    pub_type: 杂志文章


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    journal_title:Molecular neurobiology

    pub_type: 杂志文章


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    journal_title:Molecular neurobiology

    pub_type: 杂志文章,评审


    authors: Chatonnet F,Domínguez del Toro E,Thoby-Brisson M,Champagnat J,Fortin G,Rijli FM,Thaëron-Antôno C

    更新日期:2003-12-01 00:00:00

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    pub_type: 杂志文章


    authors: Zhang Y,Dong H,Seeburg DP,Wojtkiewicz GR,Waterman P,Pulli B,Forghani R,Ali M,Iwamoto Y,Swirski FK,Chen JW

    更新日期:2019-02-01 00:00:00

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    journal_title:Molecular neurobiology

    pub_type: 杂志文章


    authors: Caffino L,Piva A,Mottarlini F,Di Chio M,Giannotti G,Chiamulera C,Fumagalli F

    更新日期:2018-07-01 00:00:00

  • Prognostic Value of YKL-40 in Patients with Glioblastoma: a Systematic Review and Meta-analysis.

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    journal_title:Molecular neurobiology

    pub_type: 杂志文章,meta分析,评审


    authors: Qin G,Li X,Chen Z,Liao G,Su Y,Chen Y,Zhang W

    更新日期:2017-07-01 00:00:00

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    journal_title:Molecular neurobiology

    pub_type: 杂志文章,评审


    authors: De Blas AL

    更新日期:1996-02-01 00:00:00

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    journal_title:Molecular neurobiology

    pub_type: 杂志文章


    authors: Oliveira SR,Figueiredo-Pereira C,Duarte CB,Vieira HLA

    更新日期:2019-05-01 00:00:00

  • Immunomodulatory Effect of Toll-Like Receptor-3 Ligand Poly I:C on Cortical Spreading Depression.

    abstract::The release of inflammatory mediators following cortical spreading depression (CSD) is suggested to play a role in pathophysiology of CSD-related neurological disorders. Toll-like receptors (TLR) are master regulators of innate immune function and involved in the activation of inflammatory responses in the brain. TLR3...

    journal_title:Molecular neurobiology

    pub_type: 杂志文章


    authors: Ghaemi A,Sajadian A,Khodaie B,Lotfinia AA,Lotfinia M,Aghabarari A,Khaleghi Ghadiri M,Meuth S,Gorji A

    更新日期:2016-01-01 00:00:00

  • Sphingosine Kinase-1 Is Essential for Maintaining External/Outer Limiting Membrane and Associated Adherens Junctions in the Aging Retina.

    abstract::Sphingosine-1-phosphate (S1P) produced by sphingosine kinases (SPHK1 and SPHK2) is a signaling molecule involved in cell proliferation and formation of cellular junctions. In this study, we characterized the retinas of Sphk1 knockout (KO) mice by electron microscopy and immunocytochemistry. We also tested cultured Mül...

    journal_title:Molecular neurobiology

    pub_type: 杂志文章


    authors: Wilkerson JL,Stiles MA,Gurley JM,Grambergs RC,Gu X,Elliott MH,Proia RL,Mandal NA

    更新日期:2019-10-01 00:00:00

  • Oxytocin Neurons Enable Melanocortin Regulation of Male Sexual Function in Mice.

    abstract::The melanocortin pathway has been implicated in both metabolism and sexual function. When the melanocortin 4 receptor (MC4R) is knocked out globally, male mice display obesity, low sexual desire, and copulatory difficulties; however, it is unclear whether these phenotypes are interdependent. To elucidate the neuronal ...

    journal_title:Molecular neurobiology

    pub_type: 杂志文章


    authors: Semple E,Shalabi F,Hill JW

    更新日期:2019-09-01 00:00:00

  • Antidepressant-Like Effect of Ropren® in β-Amyloid-(25-35) Rat Model of Alzheimer's Disease with Altered Levels of Androgens.

    abstract::This study elucidated the potential antidepressant-like effect of prolonged Ropren® administration (8.6 mg/kg, orally, once daily for 28 days) using a β-amyloid (25-35) rat model of Alzheimer's disease following gonadectomy. The experimental model was created by intracerebroventricular injection of β-amyloid (25-35) i...

    journal_title:Molecular neurobiology

    pub_type: 杂志文章


    authors: Soultanov V,Fedotova J,Nikitina T,Roschin V,Ordyan N,Hritcu L

    更新日期:2017-05-01 00:00:00

  • Effects of prenatal cocaine exposure in the photoreceptor cells of the rat retina.

    abstract::Despite the increasing evidence of eye abnormalities, the effects of prenatal exposure to cocaine on the visual system are still poorly understood. This study was aimed at analyzing the qualitative and quantitative organization of the retinal photoreceptor cells (PR) and outer nuclear layer (ONL) after prenatal exposu...

    journal_title:Molecular neurobiology

    pub_type: 杂志文章


    authors: Silva-Araújo A,Abreu-Dias P,Silva MC,Tavares MA

    更新日期:1995-08-01 00:00:00