Abstract：
:This experiment's with aim was to study the pharmacokinetics of asperosaponin VI and its three metabolites (cauloside A, HN saponin F and hederagenin) via a sensitive high performance liquid chromatography connected with electrospray ionization triple quadrupole mass spectrum (HPLC-ESI-MS/MS). Chromatographic separation was achieved on a reverse phase C18 column with a gradient mobile phase of CH3CN-water with 0.1 % HCOOH at a flow rate of 0.3 mL/min. Sample analysis was simultaneously performed with a multiple reaction monitoring mode using target determination ions at m/z 927.5 → 603.4 for asperosaponin VI, m/z 811.1 → 603.4 for cauloside A, m/z 649.4 → 603.4 for HN saponin F, m/z 71.4 → 393.3 for hederagenin and m/z 307.0 → 161.1 for warfarin as the internal standard. The calibration curve was linear at the range of 0.25-500 ng/mL, and the lower limit of quantification was 0.25 ng/mL for each compound. While the precisely intra-assay and inter-assay variabilities were <9.5 and 7.8 %, respectively; accuracy was determined at the concentrations of 5, 25, 100 ng/mL for all the analytes with the relative standard deviation (%) no more than 15.0 %. Consequently, the validated method could be successfully and precisely applied to the pharmacokinetic study of asperosaponin VI and its metabolites. As a result, the pharmacokinetic parameters of cauloside A, HN saponin F and hederagenin such as T max were obtained at 9.33 ± 2.49, 7.33 ± 0.47 and 12.33 ± 2.36 h, respectively.
journal_name
J Nat Medjournal_title
Journal of natural medicinesauthors
Liu EW,Wang JL,Han LF,Chang YX,Wang T,Huo Y,Wang L,Gao XMdoi
10.1007/s11418-014-0821-4subject
Has Abstractpub_date
2014-07-01 00:00:00pages
488-97issue
3eissn
1340-3443issn
1861-0293journal_volume
68pub_type
杂志文章abstract：:Crude extract of Juniperus excelsa (JeExt), which tested positive for the presence of anthraquinone, flavonoids, saponins, sterols, terpenes and tannin, exhibited a protective effect against castor oil-induced diarrhoea in mice at 100-1000 mg/kg. In rabbit jejunum preparations, JeExt (0.01-1.0 mg/mL) caused relaxation...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-011-0605-z
更新日期：2012-04-01 00:00:00
abstract：:Activity-guided isolation of the n-butanol fraction of Chamaerhodos erecta and water soluble fraction of C. altaica resulted in the isolation of 39 compounds, including new compounds identified as 4,5-dihydroxybenzaldehyde-3-O-β-D-glucopyranoside (1) from C. erecta and quercetin-3-O-β-D-glucuronopyranosyl-4'-O-β-D-glu...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-013-0748-1
更新日期：2013-10-01 00:00:00
abstract：:Picrasma javanica Blume (Simaroubaceae) is a medium-sized tree that is distributed widely in tropical Asia. In our previous study, we isolated quassinoids from P. javanica bark collected in Myanmar, and reported their antiproliferative activities. In our ongoing research for the discovery of bioactive compounds from M...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-018-01279-z
更新日期：2019-06-01 00:00:00
abstract：:Mimosa pudica is used in traditional medicine for treating various disorders such as inflammatory conditions, diarrhoea, insomnia, alopecia, urogenital infections and wounds. The present study investigated the effect of M. pudica extract (MPE) on L-arginine-induced acute necrotising pancreatitis in rats. The ethanolic...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-016-0991-3
更新日期：2016-07-01 00:00:00
abstract：:Cancer cachexia is a complex and multifactorial syndrome that influences about 50-80% of cancer patients and may lead to 20% of cancer deaths and muscle atrophy is the key characteristic of the syndrome. Recent researches have shown that myostatin is a negative regulator in the growth and differentiation of skeletal m...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-020-01428-3
更新日期：2020-09-01 00:00:00
abstract：:Solvent partitioning followed by column chromatography of the MeOH extract of the seeds of Swietenia mahagoni afforded two limonoids, swietenolide (1) and 2-hydroxy-3-O-tigloylswietenolide (2). The compounds were identified by spectroscopic means. The antibacterial activity of these compounds was assessed against eigh...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-008-0287-3
更新日期：2009-01-01 00:00:00
abstract：:Two new limonoids, sanjecumins A (1) and B (2), have been isolated from the leaves of Sandoricum koetjape, together with sandoripins A (3) and B (4). Their structures and absolute configurations were elucidated on the basis of NMR and CD data. Sandoripins A (3) and B (4) moderately inhibited nitric oxide production in...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-012-0677-4
更新日期：2013-04-01 00:00:00
abstract：:Antihypertensive treatment is highly important to prevent the progression of chronic kidney disease. Shichimotsukokato (SKT), a traditional Japanese medicine (i.e., Kampo formula), lowered systolic blood pressure (SBP) in experimental animal models of hypertension. However, its mechanism of action has not been fully e...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-015-0945-1
更新日期：2016-04-01 00:00:00
abstract：:To date, there are very few effective drugs for liver fibrosis treatment; therefore, it is urgent to develop novel therapeutic targets and approaches. In the present research, we sought to study the protective effect of sweroside contained in Lonicera japonica or blue honeysuckle berries in a mouse model of liver fibr...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-019-01334-3
更新日期：2020-01-01 00:00:00
abstract：:Recruitment of immune cells to adipose tissue is altered dramatically in obesity, which results in chronic inflammation of the adipose tissue that leads to metabolic disorders, such as insulin resistance and type 2 diabetes mellitus. The regulation of immune cell infiltration into adipose tissue has prophylactic and t...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-019-01332-5
更新日期：2020-01-01 00:00:00
abstract：:Agarwood oil and spikenard extract were examined for their sedative activity using a spontaneous vapor administration system. It was shown that inhalation of agarwood oil vapor sedated mice. The main volatile constituents of the oil were found to be benzylacetone [agarwood oil from a Hong Kong market (1)], or alpha-gu...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-007-0177-0
更新日期：2008-01-01 00:00:00
abstract：:The root of Angelica acutiloba Kitagawa is an important crude drug in Kampo medicines (traditional Japanese medicine). Chemical evaluation of crude drugs is crucial to ensuring the safety and efficacy of herbal medicine; however, there is currently no chemical standard for the A. acutiloba crude drug in Japanese pharm...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-018-1190-1
更新日期：2018-06-01 00:00:00
abstract：:The article Comparison of glycyrrhizin content in 25 major kinds of Kampo extracts containing Glycyrrhizae Radix used clinically in Japan, written by Mitsuhiko Nose, Momoka Tada, Rika Kojima, Kumiko Nagata, Shinsuke Hisaka, Sayaka Masada, Masato Homma and Takashi Hakamatsuka, was originally published Online First with...
journal_title：Journal of natural medicines
pub_type： 杂志文章,已发布勘误
doi：10.1007/s11418-018-1195-9
更新日期：2018-03-01 00:00:00
abstract：:Nardostachys jatamansi (NJ) has been used in the treatment of inflammatory diseases. However, it is not clear how NJ produces anti-inflammatory effects. In the present study, using an experimental model of lipopolysaccharide (LPS)-induced endotoxin shock, the protective effects and mechanisms of action of NJ were inve...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-010-0458-x
更新日期：2011-01-01 00:00:00
abstract：:The present study aimed at investigating the effect of carvacrol on hepatic mitochondrial enzyme activities and DNA damage in D: -galactosamine (D: -GalN)-induced hepatotoxicity in male albino Wistar rats. The activities of hepatic mitochondrial enzymes such as isocitrate dehydrogenase, α-ketoglutarate dehydrogenase, ...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-011-0544-8
更新日期：2011-07-01 00:00:00
abstract：:Several studies have indicated the involvement of oxidative stress and high glucose-induced cell death in the development of diabetic neuropathy. Satureja khuzestanica has been recommended in the literature as a remedy for the treatment of diabetes, and also contains antioxidant agents. Here, we investigated the possi...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-012-0646-y
更新日期：2013-01-01 00:00:00
abstract：:Five polyphenolic compounds were identified from the diethyl ether and ethyl acetate extracts of the herb of ragged robin, Lychnis flos-cuculi L. (Caryophyllaceae). The identified and isolated compounds were established as a caffeic acid, luteolin, apigenin, apigenin 8-C-beta-D-glucopyranoside (vitexin) and luteolin 8...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-008-0261-0
更新日期：2008-10-01 00:00:00
abstract：:In a previous study, we found that both synthetic 3-O-methylquercetin (3MQ) and 3,4',7-O-trimethylquercetin (34'7TMQ) increased extracellular melanin content. 34'7TMQ increased the activity of melanogenic enzymes by stimulating the p38 pathway and the expression of microphthalmia-associated transcription factor (MITF)...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-018-1175-0
更新日期：2018-03-01 00:00:00
abstract：:An acyclic diterpene (plaunotol; 1) and two furanoditerpenes (plaunolide, 2 and plaunol E, 3), were isolated from Croton stellatopilosus leaves, and assessed for their inhibitory activity on nitric oxide (NO) production by lipopolysaccharide (LPS)-induced RAW264.7 cells. Plaunotol, plaunolide and plaunol E exhibited i...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-012-0668-5
更新日期：2013-01-01 00:00:00
abstract：:Six cycloartane glycosides, named tareciliosides N-S, and a known iridoid glucoside, ixoroside, were isolated in further extensive studies on the 1-BuOH-soluble fraction of a MeOH extract of the leaves of Tarenna gracilipes. From the EtOAc-soluble fraction, three known triterpenes were isolated. Their toxicities towar...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-012-0707-2
更新日期：2013-07-01 00:00:00
abstract：:Visceral obesity induces insulin resistance and is recognized as an important risk factor for metabolic syndrome (MS). Therefore, inhibition of lipid absorption from the intestine is regarded as an effective way of preventing MS. Terminalia bellirica is extensively used in Ayurvedic medicine in India and neighboring c...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-011-0606-y
更新日期：2012-07-01 00:00:00
abstract：:A new oxygenated lanostane-type triterpene, named lucidumol C, together with six known compounds, was isolated from the chloroform extract of the fruiting bodies of Ganoderma lingzhi. Structures were established based on extensive spectroscopic and chemical studies. Potential cytotoxic activities of the isolated compo...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-016-0976-2
更新日期：2016-07-01 00:00:00
abstract：:Acanthopanax senticosus HARMS (AS) is used as a Chinese herbal medicine and as a health supplement in Japan. However, little is known about the interaction between AS and other drugs. In this study, we investigated the effect of AS extract on intestinal drug transporter (P-glycoprotein, or P-gp) and peptide transporte...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-009-0371-3
更新日期：2010-01-01 00:00:00
abstract：:Fufang Xueshuantong Capsule (FXST) can reduce urinary albumin and whole blood viscosity in early diabetic nephropathy (DN) patients. This research aimed to investigate the effect of FXST on kidney function in DN rats and to identify the underlying molecular mechanisms. We performed Illumina RatRef-12 Expression BeadCh...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-012-0654-y
更新日期：2013-01-01 00:00:00
abstract：:In recent years, Spirulina has gained more and more attention from medical scientists as a nutraceutical and a source of potential pharmaceuticals. The present study was conducted to elucidate the immunomodulatory effect of Spirulina fusiformis (a cyanobacterium of the family Oscillatoriaceae) in vivo and in vitro. Th...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-008-0308-2
更新日期：2009-04-01 00:00:00
abstract：:(E)-methyl-4-hydroxy-3,5-dimethoxycinnamate, (E)-methyl-4-hydroxy-3-methoxycinnamate, (E)-methyl-4-hydroxycinnamate, (Z)-methyl-4-hydroxycinnamate, calycanthine, ursolic acid, 2-hydroxy-3-methylanthraquinone, 4-methoxybenzoic acid, 3,4,5-trimethoxybenzoic acid, methyl 4-hydroxybenzoate and allantoin were isolated from...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-008-0227-2
更新日期：2008-07-01 00:00:00
abstract：:Evodiae fructus (EF), a fruit of Evodia rutaecarpa Bentham, has long been used as an analgesic drug in traditional Chinese and Japanese medicine. However, the underlying molecular mechanism of its pharmacological action is unclear. Here, using calcium imaging, whole-cell patch-clamp recording, and behavioral analysis,...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-015-0929-1
更新日期：2016-01-01 00:00:00
abstract：:An immunoassay system was established for the estimation of the quantity of an antitumor cyclic hexapeptide RA-VII (1) from Rubia cordifolia L. and R. akane Nakai (Rubiaceae). First, 1 was converted into its hapten, which was then conjugated with a carrier protein to be used as an effective antigen to obtain its monoc...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-011-0508-z
更新日期：2011-07-01 00:00:00
abstract：:Two tetrahydroxanthones, 1,3,5S,8S-tetrahydroxy-5,6,7,8-tetrahydroxanthone (1) and 1,3,5R,8S-tetrahydroxy-5,6,7,8-tetrahydroxanthone (2), and six new tetrahydroxanthone glycosides, amarellins A-F (3-8), were isolated from the aerial parts of a Mongolian medicinal plant Gentianella amarella ssp. acuta (Gentianaceae). T...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-016-1015-z
更新日期：2016-10-01 00:00:00
abstract：:We aimed to establish a methodology for identifying Paeonia samples based on metallomic analysis. We prepared 66 batches of samples (16 batches of crude drugs and 50 batches of cultivars, comprising 64 batches of Paeonia lactiflora and 2 batches of P. veitchii) collected from Japan and China (Inner Mongolia and elsewh...
journal_title：Journal of natural medicines
pub_type： 杂志文章
doi：10.1007/s11418-013-0795-7
更新日期：2014-04-01 00:00:00
