Abstract:
:Genetic bottlenecks are important events in the genetic diversification of organisms and colonization of new ecological niches. Repeated bottlenecking of RNA viruses often leads to fitness losses due to the operation of Muller's ratchet. Herein we use vesicular stomatitis virus to determine the transmission population size which leads to fitness decreases of virus populations. Remarkably, the effective size of a genetic bottleneck associated with fitness loss is greater when the fitness of the parental population increases. For example, for starting virus populations with low fitness, population transfers of five-clone-to-five-clone passages resulted in a fitness increase. However, when a parental population with high fitness was transferred, 30-clone-to-30-clone passages were required simply to maintain fitness values.
journal_name
J Viroljournal_title
Journal of virologyauthors
Novella IS,Elena SF,Moya A,Domingo E,Holland JJdoi
10.1128/JVI.69.5.2869-2872.1995subject
Has Abstractpub_date
1995-05-01 00:00:00pages
2869-72issue
5eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
杂志文章abstract::Kaposi's sarcoma-associated herpesvirus (KSHV/human herpesvirus 8 [HHV-8]) is a gamma-2-herpesvirus responsible for Kaposi's sarcoma as well as primary effusion lymphoma (PEL). KSHV is a lymphotropic virus that has pirated many mammalian genes involved in inflammation, cell cycle control, and angiogenesis. Among these...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.57-67.2003
更新日期:2003-01-01 00:00:00
abstract:UNLABELLED:In vitro, infection of polarized human intestinal epithelial cells by coxsackievirus B3 (CVB3) depends on virus interaction with decay-accelerating factor (DAF), a receptor expressed on the apical cell surface. Although mice are highly susceptible to CVB3 infection when virus is delivered by intraperitoneal ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03468-14
更新日期:2015-04-01 00:00:00
abstract::Mucosal surfaces are the primary portals for human immunodeficiency virus (HIV) transmission. Because systemic immunization, in general, does not induce effective mucosal immune responses, a mucosal HIV vaccine is urgently needed. For this study, we developed papillomavirus pseudoviruses that express HIV-1 Gag. The ps...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10249-10257.2004
更新日期:2004-10-01 00:00:00
abstract::Because immunologic classification of human immunodeficiency virus type 1 (HIV) might be more relevant than genotypic classification for designing polyvalent vaccines, studies were undertaken to determine whether immunologically defined groups of HIV ("immunotypes") could be identified. For these experiments, the V3 r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.5.4042-4051.1999
更新日期:1999-05-01 00:00:00
abstract::The vaccinia virus Western Reserve A38L protein is a hydrophobic integral membrane glycoprotein with amino acid similarity to mammalian integrin-associated protein. The protein has an N-terminal immunoglobulin superfamily domain, followed by five membrane-spanning domains and a short cytoplasmic tail. Deletion of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.2.905-914.1996
更新日期:1996-02-01 00:00:00
abstract::Betapapillomavirus replication and transcription have not been studied in detail because of a lack of suitable cellular systems supporting human papillomavirus (HPV) genome replication. We have recently shown that the human osteosarcoma cell line U2OS provides a useful environment for the genome replication of many di...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01841-13
更新日期:2014-01-01 00:00:00
abstract::Several different forms of progeny viral DNA can be identified in polyoma virus (Py)-infected mouse L-cells. The majority comprise mature form I superhelical DNA and the circular, double-stranded "theta" replicating intermediates in which the progeny DNA strands never exceed the unit genome length of the template. The...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.3.768-777.1983
更新日期:1983-06-01 00:00:00
abstract::The human T-cell leukemia virus type 1 (HTLV-1) transforming protein, Tax, is a potent transactivator of both viral and cellular gene expression. The ability of Tax to transform cells is believed to depend on its transactivation of cellular-growth-regulatory genes. Expression of proliferating cell nuclear antigen (PCN...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1181-1190.1997
更新日期:1997-02-01 00:00:00
abstract::Although recombinant human adenovirus (HAV)-based vectors offer several advantages for somatic gene therapy and vaccination over other viral vectors, it would be desirable to develop alternative vectors with prolonged expression and decreased toxicity. Toward this objective, a replication-defective bovine adenovirus t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.11.9137-9144.1999
更新日期:1999-11-01 00:00:00
abstract::A number of cDNA clones complementary to measles virus mRNA and 50S genome RNA have been generated. These clones have been mapped by restriction enzyme analysis and were subsequently sequenced by the method of Maxam and Gilbert (A. M. Maxam and W. Gilbert, Methods Enzymol. 65:499-560, 1980). Computer analysis of these...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.1.186-193.1985
更新日期:1985-04-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) encodes specific trafficking signals within its long cytoplasmic tail (CT) that regulate incorporation into HIV-1 particles. Rab11-family interacting protein 1C (FIP1C) and Rab14 are host trafficking factors required for Env particle incorpora...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01893-17
更新日期:2018-02-12 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) UL25 gene contains a 580-amino-acid open reading frame that codes for an essential protein. Previous studies have shown that the UL25 gene product is a virion component (M. A. Ali et al., Virology 216:278-283, 1996) involved in virus penetration and capsid assembly (C. Addison e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1060-1070.1998
更新日期:1998-02-01 00:00:00
abstract::Both exponentially growing and serum-arrested subcloned CV-1 cell cultures were infected with simian virus 40 (SV40). By 24 h after infection 96% of the nuclei of these permissive cells contained SV40 T-antigen. Analysis of the average DNA content per cell at various times after infection indicated that by 24 h most o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.1.76-83.1979
更新日期:1979-04-01 00:00:00
abstract::The utility of the present generation of adenovirus (Ad) vectors for gene therapy applications could be improved by restricting native viral tropism to selected cell types. In order to achieve modification of Ad tropism, we proposed to exploit a minor component of viral capsid, protein IX (pIX), for genetic incorporat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.14.6893-6899.2002
更新日期:2002-07-01 00:00:00
abstract::Interactions between the oncogenic retrovirus human T-cell leukemia virus type 1 (HTLV-1) and dendritic cells (DCs) are poorly characterized. We show here that monocyte-derived DCs form syncytia and are infected upon coculture with HTLV-1-infected lymphocytes. We examined the role of DC-specific ICAM-3-grabbing nonint...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.10.4771-4780.2006
更新日期:2006-05-01 00:00:00
abstract::The immune response to bovine herpesvirus 1 (BHV-1) infection can protect cattle from subsequent challenge with the virus. This protection involves a variety of defensive strategies, and the activation of most of these defenses requires the recognition of viral proteins by the cellular immune system. To identify some ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.10.5114-5122.1990
更新日期:1990-10-01 00:00:00
abstract::Tomato bushy stunt virus (TBSV), a tombusvirus with a nonsegmented, plus-stranded RNA genome, codes for two essential replicase proteins. The sequence of one of the replicase proteins, namely p33, overlaps with the N-terminal domain of p92, which contains the signature motifs of RNA-dependent RNA polymerases (RdRps) i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.17.9244-9258.2003
更新日期:2003-09-01 00:00:00
abstract::The hepatitis C virus (HCV) encodes a large polyprotein; therefore, all viral proteins are produced in equimolar amounts regardless of their function. The aim of our study was to determine the ratio of nonstructural proteins to RNA that is required for HCV RNA replication. We analyzed Huh-7 cells harboring full-length...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.21.13594-13605.2005
更新日期:2005-11-01 00:00:00
abstract::Marburg virus, a filovirus, causes severe hemorrhagic fever with hitherto poorly understood molecular pathogenesis. We have investigated here the vectorial transport of the surface protein GP of Marburg virus in polarized epithelial cells. To this end, we established an MDCKII cell line that was able to express GP per...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.3.1274-1283.2001
更新日期:2001-02-01 00:00:00
abstract::Ebola virus (EBOV) infections result in aggressive hemorrhagic fever in humans, with fatality rates reaching 90% and with no licensed specific therapeutics to treat ill patients. Advances over the past 5 years have firmly established monoclonal antibody (MAb)-based products as the most promising therapeutics for treat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01548-18
更新日期:2019-02-19 00:00:00
abstract::Herpes simplex virus type 1 glycoprotein H (HSV-1 gH) was synthesized in an inducible mammalian cell expression system, and its properties were examined. The gH coding sequence, together with the stable 5' untranslated leader sequence from xenopus beta-globin, was placed under control of the strong promoter from the h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4744-4755.1989
更新日期:1989-11-01 00:00:00
abstract::PM2 DNA was prepared with different superhelical densities (sigma) in order to examine the relationship betweenn supercoiling and the occurrence of a region(s) of unpaired bases in this DNA. A previous study showed that CH3HgOH reacts with native superhelical PM2 DNA more rapidly than the nicked form II. This evaluati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.1.195-204.1976
更新日期:1976-04-01 00:00:00
abstract::Protective immunity to rotavirus (RV) is primarily mediated by antibodies produced by RV-specific memory B cells (RV-mBc). Of note, most of these cells express IgM, but the function of this subset is poorly understood. Here, using limiting dilution assays of highly sort-purified human IgM(+) mBc, we found that 62% and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01466-12
更新日期:2012-10-01 00:00:00
abstract::Kaposi sarcoma-associated herpesvirus (KSHV) is an emerging pathogen and is the causative infectious agent of Kaposi sarcoma and two malignancies of B cell origin. To date, there is no licensed KSHV vaccine. Development of an effective vaccine against KSHV continues to be limited by a poor understanding of how the vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00630-19
更新日期:2019-07-30 00:00:00
abstract::DNA sequences which have structural features suggestive of their functioning as an origin of lytic-phase DNA replication were previously identified in both human herpesvirus 6B strain Z29 [HHV-6B (Z29)] and in HHV-6A (U1102). Plasmid constructs containing the putative HHV-6B (Z29) oriLyt element were replicated after ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7680-7683.1993
更新日期:1993-12-01 00:00:00
abstract::Cellular inhibitor of apoptosis protein 2 (cIAP2) is a potent suppressor of apoptotic cell death. We have shown previously that cIAP2 is involved in the tumor necrosis factor alpha (TNF-α)-induced anti-hepatitis B virus (HBV) response; however, the mechanism for this antiviral effect remains unclear. In the present st...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00879-11
更新日期:2011-11-01 00:00:00
abstract::The murine polyomavirus (Py) enters mouse fibroblasts and kidney epithelial cells via an endocytic pathway that is caveola-independent (as well as clathrin-independent). In contrast, uptake of simian virus 40 into the same cells is dependent on caveola. Following the initial uptake of Py, both microtubules and microfi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2615-2622.2003
更新日期:2003-02-01 00:00:00
abstract::The human airway epithelium (HAE) represents the entry port of many human respiratory viruses, including human coronaviruses (HCoVs). Nowadays, four HCoVs, HCoV-229E, HCoV-OC43, HCoV-HKU1, and HCoV-NL63, are known to be circulating worldwide, causing upper and lower respiratory tract infections in nonhospitalized and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03368-12
更新日期:2013-06-01 00:00:00
abstract::Herpes simplex virus virions contain one or more functions which mediate shutoff of host protein synthesis, disaggregation of host polyribosomes, and degradation of host mRNA. We studied aspects of the host shutoff mechanism by using herpes simplex virus type 1 mutants deficient in virion-induced shutoff of host prote...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.7.2198-2207.1987
更新日期:1987-07-01 00:00:00
abstract::Virion protein 16 (VP16) of herpes simplex virus type 1 (HSV-1) is a potent transcriptional activator of viral immediate-early (IE) genes. The VP16 activation domain can recruit various transcriptional coactivators to target gene promoters. However, the role of transcriptional coactivators in HSV-1 IE gene expression ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02349-08
更新日期:2009-04-01 00:00:00