Abstract：
:Hsp47, Grp78, and Grp94 have been implicated with procollagen maturation events. In particular, Hsp47 has been shown to nascent procollagen alpha 1(I) chains in the course of synthesis and/or translocation into the endoplasmic reticulum (ER). Although, Hsp47 binding to gelatin and collagen has previously been suggested to be independent of ATP. Grp78 and Grp94 are known to dissociate from its substrates by an ATP-dependent release mechanism. The early association of Hsp47 with procollagen and its relatively late release suggested that other chaperones, Grp78 and Grp94, interact successively or concurrently with Hsp47. Herein, we examined how these events occur in cells metabolically stressed by depletion of ATP. In cells depleted of ATP, the release of Hsp47, Grp78, and Grp94 from maturing procollagen is delayed. Thus, in cells experiencing metabolic stress, newly synthesized procollagen unable to properly fold became stably bound to a complex of molecular chaperones. In that Hsp47, Grp78, and Grp94 could be recovered with nascent procollagen and as oligomers in ATP depleted cells suggests that these chaperones function in a series of coupled or successive reactions.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Ferreira LR,Norris K,Smith T,Hebert C,Sauk JJdoi
10.1002/jcb.240560412subject
Has Abstractpub_date
1994-12-01 00:00:00pages
518-26issue
4eissn
0730-2312issn
1097-4644journal_volume
56pub_type
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doi：10.1002/jcb.25528
更新日期：2016-10-01 00:00:00
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更新日期：1995-03-01 00:00:00
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更新日期：2014-02-01 00:00:00
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pub_type： 杂志文章,随机对照试验
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