Abstract:
:In the present study, monoclonal antibodies were produced using porcine adipocyte extracts as the immunogen. Two of the monoclonal antibodies, designated CB6 and IB4, exhibited reactivity toward only cells containing lipid in stromal-vascular cell cultures. The antigens recognized by the CB6 and IB4 monoclonal antibodies were 50 kD and 55 kD proteins, respectively. In vivo, IB4 immunoreactivity was detected only in lipid-containing cells, whereas immunofluorescence using CB6 was also detectable around muscle fiber bundles underlying the subcutaneous mesenchyme. In fetal subcutaneous mesenchyme, CB6 and IB4 immunoreactivities toward lipid-containing cells increased with developmental age, but each was not detectable in cells containing the smallest lipid droplets. In stromal-vascular cultures containing adipocytes, 48 hour treatment with the anti-lipogenic agent, growth hormone, only slightly altered CB6 immunoreactivity, whereas IB4 immunoreactivity was reduced by more than sixfold. The exact identity of the CB6 and IB4 antigens was not determined, but each may be useful as markers for studying regulation of adipocyte metabolism.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Wright JT,Hausman GJdoi
10.1002/jcb.240450311subject
Has Abstractpub_date
1991-03-01 00:00:00pages
284-91issue
3eissn
0730-2312issn
1097-4644journal_volume
45pub_type
杂志文章abstract::Sea urchin CS histone variants are electrophoretically heterogeneous when analyzed in two dimensional polyacrylamide gels (2D-PAGE). Previous results suggested that this heterogeneity is due to the poly (ADP-ribosylation) of these proteins. Consequently, native CS histone variants were subjected to different treatment...
journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
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更新日期:2013-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-04-15 00:00:00
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journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240400315
更新日期:1989-07-01 00:00:00
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更新日期:2018-12-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,meta分析
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