Abstract:
:Lysis of human culture-derived macrophages by extracellular ATP has recently been described, and treatment of macrophages with interferon-gamma rendered those cells significantly more sensitive to lysis. In addition, cell death occurred more rapidly in interferon (IFN)-treated cells than in untreated macrophages. In an attempt to identify the mechanism by which extracellular ATP affects macrophages, as well as to explore the differences between interferon-gamma-treated and untreated macrophages, selected metabolic inhibitors were included in the lytic assays. Of the compounds tested, three antagonists of calmodulin-linked pathways (trifluoperazine, KN-62, and calmidazolium) blocked the ATP-mediated lysis of both interferon-gamma-treated and colony-stimulating factor-treated macrophages in a dose-dependent manner. Early signals of the ATP ligation of the P2Z purinoceptors of human macrophages included increases in cytosolic [Ca2+] and depolarization of the plasma membrane. However, the inclusion of calmodulin antagonists in these assays did not abrogate either effect. These results suggest that the mechanism which mediates the efflux of 51Cr-labeled proteins from ATP-lysed macrophages is distinct from calcium mobilization and membrane depolarization, and may involve the generation of secondary pores/channels in the plasma membrane via a calmodulin-linked pathway.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Blanchard DK,Hoffman SL,Djeu JYdoi
10.1002/jcb.240570311subject
Has Abstractpub_date
1995-03-01 00:00:00pages
452-64issue
3eissn
0730-2312issn
1097-4644journal_volume
57pub_type
杂志文章abstract:OBJECTIVE:The current study was designed to investigate the inhibitory effects of ginsenoside Rd (Gs-Rd) on human glioma U251 cells in vitro and its possible underlying mechanisms. METHODS:The groups included blank control group, low concentration Gs-Rd treatment group (20 μM), mid concentration Gs-Rd treatment group ...
journal_title:Journal of cellular biochemistry
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更新日期:2019-03-01 00:00:00
abstract::Cervical cancer is one of the leading killers for female worldwide. Nevertheless, the less knowledge of molecular mechanism for cervical cancer limited the improvement of treatment effects. High-mobility group box 2 (HMGB2) belongs to the HMGB family, which could play diverse roles in cell proliferation. This work mai...
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abstract::Growing evidence has shown that a large number of miRNAs are abnormally expressed in cervical cancer (CC) tissues and play irreplaceable roles in tumorigenesis, progression, and metastasis. This study aimed to identify new biomarkers and pivotal genes associated with CC prognosis through comprehensive bioinformatics a...
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journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
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abstract::Bone marrow macrophages fuse on the bone surface to form multinucleated osteoclasts that then organize to efficiently resorb bone. Many, if not all, of the stages of macrophage fusion involve cytoskeletal components that reorganize the cells. Recruitment may involve chemotactic responses to bone matrix protein and cal...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.22640
更新日期:2010-08-01 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,随机对照试验
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更新日期:2018-11-01 00:00:00
abstract::Anoikis is a form of programmed cell death induced by loss of contact from neighboring cells or from their extracellular matrix (ECM). Many tumorigenic cells are anoikis resistant, facilitating cancer progression and metastasis. Trastuzumab is a monoclonal antibody used for the treatment of breast and gastric cell can...
journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240570410
更新日期:1995-04-01 00:00:00
abstract::Butyrate inhibits growth of lung cancer. However, the molecular mechanism is still unclear. Here we profiled miRNAs that responded to sodium butyrate(NaB) stimulation in A549 cells, a non-small cell lung cancer cell line, using microarray. We found 33 up-regulated microRNAs and 22 down-regulated microRNAs (log2 ≥1.5 f...
journal_title:Journal of cellular biochemistry
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doi:10.1002/jcb.26547
更新日期:2018-04-01 00:00:00
abstract::Wedelolactone is an herbal medicine that is used to treat septic shock, hepatitis and venom poisoning. Although in differentiated and cancer cells, wedelolactone has been identified as anti-inflammatory, growth inhibitory, and pro-apoptotic, the effects of wedelolactone on stem cell differentiation remain largely unkn...
journal_title:Journal of cellular biochemistry
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更新日期:2012-11-01 00:00:00
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journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
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更新日期:2001-04-02 00:00:00
abstract::Ebselen (2-phenyl-1,2-benzisoselenazol-3(2H)-one) is an organoselenium radical scavenger compound, which has strong antioxidant and anti-inflammatory effects. However, evidence suggests that this compound could exert deleterious actions on cell physiology. In this study, we have analyzed the effect of ebselen on rat p...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
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更新日期:2018-01-01 00:00:00
abstract::Adult human mesenchymal stem cells (MSCs) hold promise for an increasing list of therapeutic uses due to their ease of isolation, expansion, and multi-lineage differentiation potential. To maximize the clinical potential of MSCs, the underlying mechanisms by which MSC functionality is controlled must be understood. We...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
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更新日期:2009-10-15 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
abstract::This study aimed to elucidate the precise mechanisms underlying the protective effects of phosphoseryl-tRNA kinase (PSTK) against cisplatin-induced podocyte injury. PSTK overexpression and knockdown vectors were generated and transfected into murine podocyte cells-5. PSTK levels were measured, and transcriptome sequen...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.26076
更新日期:2019-06-01 00:00:00
abstract::Inhibition of metabolic features which distinguish cancer cells from their non-malignant counterparts is a promising approach to cancer treatment. Energy support for drug extrusion in multidrug resistance (MDR) is a potential target for metabolic inhibition. Two major sources of ATP-based metabolic energy are partial ...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.25247
更新日期:2016-01-01 00:00:00
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journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,评审
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更新日期:2008-02-01 00:00:00
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journal_title:Journal of cellular biochemistry
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更新日期:2019-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-09-01 00:00:00