Abstract:
:Numerous studies have demonstrated the role of A3 adenosine receptor (A3AR) and signaling pathways in the multiple aspects of the tumor. However, there is a little study about the function of A3AR in the biological processes of cancer stem cells (CSCs). CSCs have a critical role in the maintenance and survival of breast cancer. The aim of current study was to investigate the effect of A3AR agonist on breast cancer stem cells (BCSCs). XTT assay showed antiproliferative effect of A3AR agonist (Cl-IB-MECA) on BCSCs. Our results also demonstrated that A3AR agonist reduces mammosphere formation in a dose-dependent manner. Flow cytometry analysis showed that A3AR agonist induces G1 cell cycle arrest and apoptosis in BCSCs. Western blot assay showed that A3AR agonist inhibits the expression of cell cycle and apoptotic regulatory proteins as well as the expression of ERK1/2 and GLI-1 proteins. Finally, these findings propose that A3AR agonist induces cell cycle arrest and apoptosis in BCSCs by inhibition of ERK1/2 and GLI-1 cascade. J. Cell. Biochem. 118: 2909-2920, 2017. © 2017 Wiley Periodicals, Inc.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Jafari SM,Panjehpour M,Aghaei M,Joshaghani HR,Enderami SEdoi
10.1002/jcb.25945subject
Has Abstractpub_date
2017-09-01 00:00:00pages
2909-2920issue
9eissn
0730-2312issn
1097-4644journal_volume
118pub_type
杂志文章abstract::The growth of capillary endothelial cells (BCE) is an important regulatory step in the formation of capillary blood vessels. In vivo, the proliferation of these cells is stringently controlled. In vitro they can be stimulated by polypeptide growth factors, such as acidic fibroblast growth factor (aFGF) and basic fibro...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240470209
更新日期:1991-10-01 00:00:00
abstract::MicroRNAs (miRNAs) play an important role in the development and progression of non-small cell lung cancer (NSCLC). Recently, several studies have shown that miR-99a is downregulated in various cancers, which can affect tumor initiation and maintenance. Herein, we found that miR-99a was downregulated in NSCLC tissues ...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.24965
更新日期:2015-02-01 00:00:00
abstract::Relatively little information exists on the ultimate molecular mechanisms by which the lipogenic enzyme Fatty Acid Synthase (FAS) is differentially overexpressed in a biologically aggressive subset of human malignancies. Since the microenvironment of solid tumors contains regions of poor oxygenation and high acidity, ...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.20310
更新日期:2005-01-01 00:00:00
abstract::Epithelial-to-mesenchymal transition (EMT) is a major process to regulate cell migration and invasion. Inhibition of epidermal growth factor receptor (EGFR)-mediated EMT by tyrosine kinase inhibitors (TKIs) is a strategy to prevent lung cancer invasion. However, drug resistance is emerged and accelerated invasion thro...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.26128
更新日期:2017-12-01 00:00:00
abstract::In the present study, we have investigated dexamethasone and insulin regulation of the expression of adipose-specific mRNA, namely, glycerophosphate dehydrogenase (G3PDH) and adipsin, at different stages of differentiation. During adipose conversion, insulin promotes an accumulation of G3PDH mRNA which is linked to ce...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240420407
更新日期:1990-04-01 00:00:00
abstract::Recent ultrastructural, immunoelectron, and confocal microscopy observations done in our laboratory [Barboro et al. [2002] Exp. Cell. Res. 279:202-218] have confirmed that lamins and the nuclear mitotic apparatus protein (NuMA) are localized inside the interphase nucleus in a polymerized form. This provided evidence o...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1002/jcb.10378
更新日期:2003-01-01 00:00:00
abstract::The P-glycoprotein (p170, P-gp) encoded by human MDR1 gene functions as a pump to extrude anticancer drugs from cancer cells. Over-expression of p170 is closely related to primary and induced drug resistance phenotype of tumor cells. Recent studies have demonstrated that expression of cyclooxygenase-2 (COX-2) is posit...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.22239
更新日期:2009-09-01 00:00:00
abstract::We recently demonstrated the activation of extracellular signal- regulated protein kinase 1 and 2 (ERK1 and ERK2) by IGF-1, FGF-2, and PDGF-BB in normal human osteoblastic (HOB) cells as well as in rat and mouse osteoblastic cells. In this report, we have examined whether c-Jun NH2-Terminal Kinase (JNK) pathway is act...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::Melanoma is a disease which has been shown to be responsive to immune intervention. This has been suggested by reports of spontaneous responses of metastatic disease with strong immune infiltrates, and supported by recent data correlating clinical response after IFNalpha treatment with development of generalized autoi...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1002/jcb.21473
更新日期:2007-10-01 00:00:00
abstract::Membrane vesicles were prepared by incubation of human erythrocytes with dimyristoylphosphatidylcholine [3] and isolated by isopycnic centrifugation on Dextran density gradients. Protein analyses were carried out with crossed immunoelectrophoresis and dodecylsulfate polyacrylamide gel electrophoresis. The right-side-o...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240190208
更新日期:1982-01-01 00:00:00
abstract::Homocysteine-thiolactone (HcyT) is a toxic product whose synthesis is directly proportional to plasma homocysteine (Hcy) levels. Previous studies demonstrated that the interaction between HcyT and low density lipoproteins (LDL) induces the formation of homocystamide-LDL adducts (Hcy-LDL). Structural and functional alt...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.20069
更新日期:2004-05-15 00:00:00
abstract::We have partially purified and characterized an 87 kDa gelatinase activity expressed in later stage sea urchin embryos. Cleavage activity was specific for gelatin and no cleavage of sea urchin peristome type I collagen, bovine serum albumin or casein was detected. Magnesium and Zn2+ inhibited the gelatinase and Ca2+ p...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract::The three ingredients for the successful tissue engineering of bone and cartilage are regulatory signals, cells, and extracellular matrix. Recent advances in cellular and molecular biology of the growth and differentiation factors have set the stage for a symbiosis of biotechnology and biomaterials. Recent advances pe...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1002/jcb.240560213
更新日期:1994-10-01 00:00:00
abstract::The present study aimed to investigate the long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) involved in the progression of gallbladder cancer and explore the potential physiopathologic mechanisms of gallbladder cancer in terms of competing endogenous RNAs (ceRNAs). The original lncRNA and mRNA expression profi...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.28953
更新日期:2019-12-01 00:00:00
abstract::Transforming growth factor-beta (TGF-beta) increases steady-state mRNA levels of several extracellular matrix proteins in mineralized connective tissues. Bone sialoprotein (BSP) is a major constituent of the bone matrix, thought to initiate and regulate the formation of mineral crystals. To determine the molecular pat...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/(sici)1097-4644(19970615)65:4<501::aid-jcb
更新日期:1997-06-15 00:00:00
abstract::The mass and function of bones depend on the maintenance of a complicated balance between osteoclast-mediated bone resorption and osteoblast-mediated bone formation. An inhibitor of osteoclast differentiation and/or function is expected to be useful for treatment of bone lytic diseases such as osteoporosis, rheumatoid...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.23213
更新日期:2012-02-01 00:00:00
abstract::An important aspect of the cellular differentiation in the intestine is the migration of epithelial cells from the crypt to the villus tip. As homeodomaine transcription factor CDX2 has been suggested to influence cell migration, we performed a genome-wide promoter analysis for CDX2 binding in the differentiated human...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.22490
更新日期:2010-04-15 00:00:00
abstract::The active vitamin D metabolite 1,25-dihydroxyvitamin D3 [1,25-D3] is thought to promote many of its actions through interaction with a specific intracellular receptor. The discovery of such receptors in monocytes and activated lymphocytes has led investigators to evaluate the role of the hormone on the immune system....
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1002/jcb.240490106
更新日期:1992-05-01 00:00:00
abstract::As an important tumor suppressor, programmed cell death 4 (PDCD4) influences transcription and translation of multiple genes, and modulates different signal transduction pathways. However, the upstream regulation of this gene is largely unknown. In this study, we found that microRNA-182 (miRNA-182, miR-182) was upregu...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.24488
更新日期:2013-07-01 00:00:00
abstract::Curcumin is a dietary polyphenol and a bioactive phytochemical that possesses anti-inflammatory, antioxidant, anticancer, and chemopreventive properties, which make it capable of affecting multiple sites along the stem cell pathways to induce apoptosis in these cells. Curcumin's function is through suppression of cyto...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,评审
doi:10.1002/jcb.27757
更新日期:2019-04-01 00:00:00
abstract::The study was designed to elucidate the regulatory mechanism of long noncoding RNA human metastasis associated lung adenocarcinoma transcript 1 (MALAT1) in lipopolysaccharides (LPS)-caused inflammation injury in human lung fibroblasts WI-38. WI-38 cells were stimulated with LPS to construct acute pneumonia cell model....
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章,收录出版
doi:10.1002/jcb.29429
更新日期:2020-02-01 00:00:00
abstract::Hsp47, Grp78, and Grp94 have been implicated with procollagen maturation events. In particular, Hsp47 has been shown to nascent procollagen alpha 1(I) chains in the course of synthesis and/or translocation into the endoplasmic reticulum (ER). Although, Hsp47 binding to gelatin and collagen has previously been suggeste...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240560412
更新日期:1994-12-01 00:00:00
abstract::Atherosclerosis is a chronic immuno-inflammatory disease associated with blood lipids disorder. Many studies have demonstrated that caloric restriction (CR) can prevent atherosclerosis and extend lifespan. Sir2 protein, mammal's SIRT1, has been reported to at least partly contribute to the protective effect of CR. Hen...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.22240
更新日期:2009-09-01 00:00:00
abstract::The stimulation of cultured guinea pig alveolar macrophages by the chemotactic peptide N-formyl-L-methionyl-L-leucyl-L-phenylalanine, or by the phospholipid inflammatory mediator platelet activating factor (PAF) induced an increase in arachidonic acid release and its cyclooxygenase products. This release, which was mi...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240400204
更新日期:1989-06-01 00:00:00
abstract::Insulin receptor was examined as a substrate for the multipotential protein kinase casein kinase I. Casein kinase I phosphorylated partially purified insulin receptor from human placenta as shown by immunoprecipitation of the complex with antiserum to the insulin receptor. Analysis of the phosphorylated complex by pol...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240280208
更新日期:1985-01-01 00:00:00
abstract::1alpha,25-dihydroxyvitamin D(3) (1alpha,25(OH)(2)D(3)), the active metabolite of vitamin D, mediates many of its effects through the intranuclear vitamin D receptor (VDR, NR1I1), that belongs to the large superfamily of nuclear receptors. Vitamin D receptor can directly regulate gene expression by binding to vitamin D...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::We have incorporated antibodies against fibronectin or laminin into liposomes and studied their interaction with insoluble forms of these antigens. The antibodies, after modification by palmitoylchloride, were incorporated into the lipid bilayer by the cholate dialysis method. The antibodies in the liposomes recognize...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.240280105
更新日期:1985-01-01 00:00:00
abstract::One of the most frequent chromosomal translocation found in patients with acute myeloid leukemia (AML) is the t(8;21). This translocation involves the RUNX1 and ETO genes. The breakpoints regions for t(8;21) are located at intron 5 and intron 1 of the RUNX1 and ETO gene respectively. To date, no homologous sequences h...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.24605
更新日期:2013-11-01 00:00:00
abstract::Oxoglutarate receptor 1 (OXGR1), as one of the intermediates in G protein-coupled receptors (GPCRs), plays a crucial role in the citric acid cycle receptor of α-ketoglutarate and metabolism. GPCR can control the cell proliferation by regulating the downstream signaling of G protein signaling pathways. The PI3K/AKT pat...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.27970
更新日期:2019-04-01 00:00:00
abstract::Alkaptonuria (AKU) is a rare genetic disease associated with the accumulation of homogentisic acid (HGA) and its oxidized/polymerized products in connective tissues up to the deposition of melanin-like pigments (ochronosis). Since little is known on the effects of HGA and its metabolites on articular cells, we carried...
journal_title:Journal of cellular biochemistry
pub_type: 杂志文章
doi:10.1002/jcb.22780
更新日期:2010-11-01 00:00:00