Choline and methionine regulate lipid metabolism via the AMPK signaling pathway in hepatocytes exposed to high concentrations of nonesterified fatty acids.

Abstract:

:High concentrations of nonesterified fatty acids (NEFAs) and β-hydroxybutyric acid (BHBA) induce lipid peroxidation, resulting in liver damage. Choline and methionine (Met) can promote energy balance and benefit liver health in transition dairy cows; however, the regulating mechanism remains unclear. In the present study, we established the hepatocyte damage model by 1.5 mM NEFAs or BHBA treatment, and examined lipid metabolism in hepatocytes. The results showed that 1.5 mM NEFAs and 1.5 mM BHBA significantly decreased the messenger RNA (mRNA) expression of AMP-activated protein kinase (AMPK)-α as well as its target genes carnitine palmitoyltransferase-1α (CPT-1α), acetyl-CoA carboxylase, fatty acid synthetase, and Apolipoprotein B100 (ApoB100). Choline and Met upregulated the phosphorylation level of AMPK-α, which was blocked by BML (an AMPK-α inhibitor). The mRNA expression level of peroxisome proliferator-activated receptor-α (PPAR-α), CPT-1α, and ApoB100 showed a similar trend. The expressions of liver X recptoer α (LXR-α) and sterol regulatory element-binding protein 1c (SREBP-1c) were decreased by choline and Met, while only the decrease of LXR-α was blocked by BML. These findings indicate that the high-level NEFAs and BHBA weaken the lipid metabolism by impairing the fatty acid oxidation, synthesis, and transport proteins. Choline and Met regulate PPAR-α and LXR-α transcriptional activity through AMPK-α phosphorylation and regulate SREBP-1c independently of AMPK-α to promote lipid oxidation and transport in NEFAs-treated hepatocytes.

journal_name

J Cell Biochem

authors

Shen J,Sun B,Yu C,Cao Y,Cai C,Yao J

doi

10.1002/jcb.29494

subject

Has Abstract

pub_date

2019-11-03 00:00:00

eissn

0730-2312

issn

1097-4644

pub_type

杂志文章
  • The role of regulatory components from resident T lymphocytes in polyclonal B cell activation.

    abstract::Resident T lymphocytes have been found to exert helper and suppressor regulatory influences with regard to polyclonal activation of murine splenic B lymphocytes elicited by lipopolysaccharide. In the normal adult spleen, only T cell helper influences are exercised over polyclonal B cell activation. This activity is a ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.1982.240180402

    authors: Goodman MG,Weigle WO

    更新日期:1982-01-01 00:00:00

  • Oxidative stress signalling in the apoptosis of Jurkat T-lymphocytes.

    abstract::The pathways of transduction of oxidative stress signals have been studied using the Jurkat T cell model. The oxidative stress was induced by exposure of the cells to 100 microM H(2)O(2). DNA damage was detected within 15 min after commencement of treatment. DNA damage repair occurred within about 1 h in cells exposed...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.1158

    authors: Chiaramonte R,Bartolini E,Riso P,Calzavara E,Erba D,Testolin G,Comi P,Sherbet GV

    更新日期:2001-01-01 00:00:00

  • DJ1 and microRNA-214 act synergistically to rescue myoblast cells after ischemia/reperfusion injury.

    abstract::Ischemia/reperfusion injury is a tissue injury occurring post-reperfusion of tissues with pre-existing ischemia. A good blood supply to tissues aids in the survival of ischemic tissue, however, due to prolonged ischemia the levels of ATP decrease and pH declines leading to acidosis. Reduced ATP leads to an increase in...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26842

    authors: Ghaderi S,Alidadiani N,SoleimaniRad J,Heidari HR,Dilaver N,Heim C,Ramsperger-Gleixner M,Baradaran B,Weyand M

    更新日期:2018-09-01 00:00:00

  • Membrane dynamics of γ-secretase with the anterior pharynx-defective 1B subunit.

    abstract::The four-subunit protease complex γ-secretase cleaves many single-pass transmembrane (TM) substrates, including Notch and β-amyloid precursor protein to generate amyloid-β (Aβ), central to Alzheimer's disease. Two of the subunits anterior pharynx-defective 1 (APH-1) and presenilin (PS) exist in two homologous forms AP...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29832

    authors: Dehury B,Kepp KP

    更新日期:2021-01-01 00:00:00

  • S6 kinase 2 is bound to chromatin-nuclear matrix cellular fractions and is able to phosphorylate histone H3 at threonine 45 in vitro and in vivo.

    abstract::The activity of S6 kinases (S6K) is highly induced in cancer cells highlighting an essential role in carcinogenesis. The S6K family has two members: S6K1 and S6K2 which bear common as well as distinct features. In an attempt to identify S6K2 unique sequence features compared to S6K1, we applied extensive bioinformatic...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24566

    authors: Ismail HM,Hurd PJ,Khalil MI,Kouzarides T,Bannister A,Gout I

    更新日期:2014-06-01 00:00:00

  • Identification of novel inhibitors against UDP-galactopyranose mutase to combat leishmaniasis.

    abstract::Leishmania, a protozoan parasite that causes leishmaniasis, affects 1-2 million people every year worldwide. Leishmaniasis is a vector born disease and characterized by a diverse group of clinical syndromes. Current treatment is limited because of drug resistance, high cost, poor safety, and low efficacy. The urgent n...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26433

    authors: Kashif M,Tabrez S,Husein A,Arish M,Kalaiarasan P,Manna PP,Subbarao N,Akhter Y,Rub A

    更新日期:2018-03-01 00:00:00

  • Protein kinase Cε targets respiratory chain and mitochondrial membrane potential but not F0 F1 -ATPase in renal cells injured by oxidant.

    abstract::We have previously shown that protein kinase Cε (PKCε) is involved in mitochondrial dysfunction in renal proximal tubular cells (RPTC). This study examined mitochondrial targets of active PKCε in RPTC injured by the model oxidant tert-butyl hydroperoxide (TBHP). TBHP exposure augmented the levels of phosphorylated (ac...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27256

    authors: Nowak G,Bakajsova-Takacsova D

    更新日期:2018-11-01 00:00:00

  • In vitro toxicology evaluation of pharmaceuticals using Raman micro-spectroscopy.

    abstract::Raman micro-spectroscopy combined with multivariate analysis was employed to monitor real-time biochemical changes induced in living cells in vitro following exposure to a pharmaceutical. The cancer drug etoposide (topoisomerase II inhibitor) was used to induce double-strand DNA breaks in human type II pneumocyte-like...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20884

    authors: Owen CA,Selvakumaran J,Notingher I,Jell G,Hench LL,Stevens MM

    更新日期:2006-09-01 00:00:00

  • Signaling function of PSGL-1 in neutrophil: tyrosine-phosphorylation-dependent and c-Abl-involved alteration in the F-actin-based cytoskeleton.

    abstract::P-selectin glycoprotein ligand-1 (PSGL-1) is the best-characterized selectin ligand that has been demonstrated to mediate leukocytes rolling on endothelium and leukocytes recruitment into inflamed tissue in vivo. In addition to its direct role in leukocyte capturing, PSGL-1 also functions as a signal-transducing recep...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20213

    authors: Ba X,Chen C,Gao Y,Zeng X

    更新日期:2005-02-01 00:00:00

  • LncRNA Erbb4-IR promotes esophageal squamous cell carcinoma (ESCC) by downregulating miR-145.

    abstract::Erbb4-IR is a recently identified lncRNA with pivotal functions in renal injury. The present study investigated the roles of Erbb4-IR in esophageal squamous cell carcinoma (ESCC). It was observed that Erbb4-IR was upregulated in tumor tissues of patients with ESCC. Plasma levels of Erbb4-IR in patients with ESCC were ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29023

    authors: Zhang Y,Zhang L,Wang R,Wang B,Hua P,Li J

    更新日期:2019-10-01 00:00:00

  • Moringa oleifera Gold Nanoparticles Modulate Oncogenes, Tumor Suppressor Genes, and Caspase-9 Splice Variants in A549 Cells.

    abstract::Gold nanoparticles (AuNP's) facilitate cancer cell recognition and can be manufactured by green synthesis using nutrient rich medicinal plants such as Moringa oleifera (MO). Targeting dysregulated oncogenes and tumor suppressor genes is crucial for cancer therapeutics. We investigated the antiproliferative effects of ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25528

    authors: Tiloke C,Phulukdaree A,Anand K,Gengan RM,Chuturgoon AA

    更新日期:2016-10-01 00:00:00

  • Inhibitory effect of uremic solutions on protein-DNA-complex formation of the vitamin D receptor and other members of the nuclear receptor superfamily.

    abstract::Chronic renal failure is often associated with a resistance to the biologically active form of vitamin D(3), the nuclear hormone 1alpha, 25-dihydroxyvitamin D(3) (VD). The actions of VD are mediated by the vitamin D receptor (VDR), a ligand-dependent transcription factor that binds as a dimeric complex with the retino...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4644(19990901)74:3<386::aid-jcb

    authors: Toell A,Degenhardt S,Grabensee B,Carlberg C

    更新日期:1999-09-01 00:00:00

  • What are the characteristics of cycling cells in the adult central nervous system?

    abstract::Many regions of the adult central nervous system contain cycling cells. Such cells comprise a relatively small fraction of the total population of the CNS. Work over decades has attempted to determine the normal fates of these cells and their fates under pathological conditions. The recent interest in "stem" cells and...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.10381

    authors: Goldman JE

    更新日期:2003-01-01 00:00:00

  • Nucleocytoplasmic shuttling of phospholipase C-delta1: a link to Ca2+.

    abstract::Phosphoinositides (PIs) and proteins involved in the PI signaling pathway are distributed in the nucleus as well as at the plasma membrane and in the cytoplasm, although their nuclear localization mechanisms have not been clarified in detail. Generally, proteins that shuttle between the cytoplasm and nucleus contain n...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.20677

    authors: Yagisawa H

    更新日期:2006-02-01 00:00:00

  • Oxidation and nuclear localization of thioredoxin-1 in sparse cell cultures.

    abstract::Reactive oxygen species (ROS) were once viewed only as mediators of toxicity, but it is now recognized that they also contribute to redox signaling through oxidation of specific cysteine thiols on regulatory proteins. Cells in sparse cultures have increased ROS relative to confluent cultures, but it is not known wheth...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21762

    authors: Spielberger JC,Moody AD,Watson WH

    更新日期:2008-08-01 00:00:00

  • Association of vanadate-sensitive Mg(2+)-ATPase and shape change in intact red blood cells.

    abstract::Intact human erythrocytes, initially depleted of Mg2+ by EDTA incubation in the presence of A23187, exhibit Mg(2+)-dependent phosphate production of around 1.5 mmol per liter cells.h, half-maximally activated at around 0.4 mM added free Mg2+. This appears to correspond to Mg(2+)-stimulated adenosine triphosphatase (Mg...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240460403

    authors: Xu YH,Lu ZY,Conigrave AD,Auland ME,Roufogalis BD

    更新日期:1991-08-01 00:00:00

  • Simultaneous contraction and buckling of stress fibers in individual cells.

    abstract::It has been proposed that buckling of actin stress fibers (SFs) may be associated with their disassembly. However, much of the detail remains unknown partly because the use of an elastic membrane sheet, conventionally necessary for inducing SF buckling with a mechanical compression to adherent cells, may limit high qu...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.23410

    authors: Deguchi S,Matsui TS,Sato M

    更新日期:2012-03-01 00:00:00

  • Retraction: "Over-expression of FoxM1 leads to epithelial-mesenchymal transition and cancer stem cell phenotype in pancreatic cancer cells" by Bao et al.

    abstract::The above article, published online on April 18, 2011 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor in Chief, Gary S. Stein, and Wiley Periodicals, Inc. The retraction has been agreed following an investigation from Wayne State University involving the sec...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,撤回出版物

    doi:10.1002/jcb.25588

    authors:

    更新日期:2016-08-01 00:00:00

  • Alpha 4 integrin increases anoikis of human osteosarcoma cells.

    abstract::Cell motility, growth, and proliferation are regulated by adhesion to the extracellular matrix. Detachment of adherent cells from extracellular matrix results in induction of apoptosis ("anoikis"). Transformed cells often show an anchorage-independent growth that enables them to acquire a motile, invasive phenotype. T...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10465

    authors: Marco RA,Díaz-Montero CM,Wygant JN,Kleinerman ES,McIntyre BW

    更新日期:2003-04-01 00:00:00

  • Adsorption of glycosaminoglycans to the cell surface is responsible for cellular donnan effects.

    abstract::In previous publications, we showed that extracellular glycosaminoglycans reduced the membrane potential, caused cell blebbing and swelling and decreased the intracellular pH independently of cell surface receptors. These phenomena were explained by Donnan effects. The effects were so large that they could not be attr...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24791

    authors: Hagenfeld D,Kathagen N,Prehm P

    更新日期:2014-07-01 00:00:00

  • Differentiation in Acanthamoeba castellanii is induced by specific monoclonal antibodies.

    abstract::Monoclonal antibodies that bind a large molecular weight plasma membrane protein of Acanthamoeba castellanii cause the cells to differentiate. A different monoclonal antibody that binds specifically to the major plasma membrane protein has no effect upon cell division or differentiation. The induction of differentiati...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240290410

    authors: Villemez CL,Carlo PL,Russell MA

    更新日期:1985-01-01 00:00:00

  • Lysyl oxidase: properties, specificity, and biological roles inside and outside of the cell.

    abstract::Lysyl oxidase (LO) plays a critical role in the formation and repair of the extracellular matrix (ECM) by oxidizing lysine residues in elastin and collagen, thereby initiating the formation of covalent crosslinkages which stabilize these fibrous proteins. Its catalytic activity depends upon both its copper cofactor an...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.10413

    authors: Kagan HM,Li W

    更新日期:2003-03-01 00:00:00

  • Role of cell signalling involved in induction of apoptosis by benzo[a]pyrene and cyclopenta[c,d]pyrene in Hepa1c1c7 cells.

    abstract::The reactive metabolites of benzo[a]pyrene (B[a]P) and cyclopenta[c,d]pyrene (CPP) induced an accumulation/phosphorylation of p53 in Hepa1c1c7 cells, whereas inhibition of p53 reduced the apoptosis. Judged by the inhibiting effect of wortmannin, phosphatidyl-inositol-3 (PI-3) kinases such as DNA-dependent protein kina...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20251

    authors: Solhaug A,Refsnes M,Holme JA

    更新日期:2004-12-15 00:00:00

  • The alpha1 subunit of GABAA receptor is repressed by c-myc and is pro-apoptotic.

    abstract::The c-myc oncoprotein plays a critical role in the regulation of cellular proliferation and apoptosis. To mediate these biological functions, a variety of target genes are activated or repressed by c-myc, but few genes have yet been identified that directly mediate c-myc's role in proliferation or apoptosis. During a ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20708

    authors: Vaknin UA,Hann SR

    更新日期:2006-04-01 00:00:00

  • Novel leptin receptor signaling mutants identify location and sex-dependent modulation of bone density, adiposity, and growth.

    abstract::Leptin, a hormone primarily produced by adipocytes, contributes to the regulation of bone health by modulating bone density, growth and adiposity. Upon leptin binding, multiple sites of the long form of the leptin receptor (LepRb) are phosphorylated to trigger activation of downstream signaling pathways. To address th...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27726

    authors: McCabe IC,Fedorko A,Myers MG Jr,Leinninger G,Scheller E,McCabe LR

    更新日期:2019-03-01 00:00:00

  • Immature and mature megakaryocytes enhance osteoblast proliferation and inhibit osteoclast formation.

    abstract::Recent data suggest that megakaryocytes (MKs) play a role in skeletal homeostasis. In vitro and in vivo data show that MKs stimulate osteoblast (OB) proliferation and inhibit osteoclast (OC) formation, thus favoring net bone deposition. There are several mouse models with dysregulated megakaryopoiesis and resultant hi...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22456

    authors: Ciovacco WA,Cheng YH,Horowitz MC,Kacena MA

    更新日期:2010-03-01 00:00:00

  • Importance of autophosphorylation at Ser186 in the A-loop of salt inducible kinase 1 for its sustained kinase activity.

    abstract::Autophosphorylation is an important mechanism by which protein kinases regulate their own biological activities. Salt inducible kinase 1 (SIK1) is a regulator in the feedback cascades of cAMP-mediated gene expression, while its kinase domain also features autophosphorylation activity. We provide evidence that Ser186 i...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21737

    authors: Hashimoto YK,Satoh T,Okamoto M,Takemori H

    更新日期:2008-08-01 00:00:00

  • Effect of LPS on basal and induced apo E secretion by 25-OH chol and 9cRA in differentiated CaCo-2.

    abstract::The infection and inflammation process is associated with disturbances in lipid and lipoprotein metabolism. The apolipoprotein E (apo E) plays an important role in the lipoprotein metabolism and has been linked to inflammatory disease such as atherosclerosis and Alzheimer disease. An anti-inflammatory effect has also ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10786

    authors: Ripollés Piquer B,Nazih H,Neunlist M,Huvelin JM,Bard JM

    更新日期:2004-03-01 00:00:00

  • Sex-specific effects of estrogen and androgen on gene expression in human monocyte-derived osteoclasts.

    abstract::Estrogen and androgen are both critical for the maintenance of bone, but the target cells, mechanisms, and responses could be sex-specific. To compare sex-specific actions of estrogen and androgen on osteoclasts, human peripheral blood mononuclear precursor cells from adult Caucasian males (n = 3) and females (n = 3) ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.23297

    authors: Wang J,Stern PH

    更新日期:2011-12-01 00:00:00

  • Identification of the chick neural retina cell surface N-acetylgalactosaminyltransferase using monoclonal antibodies.

    abstract::Intact embryonic chick neural retina cells have at their surface an N-acetylgalactosaminyltransferase which catalyzes the incorporation of N-acetylgalactosamine from UDP-N-acetylgalactosamine into endogenous macromolecular acceptors. The enzyme along with its endogenous acceptors can be isolated as a particulate compl...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240320205

    authors: Balsamo J,Pratt RS,Emmerling MR,Grunwald GB,Lilien J

    更新日期:1986-01-01 00:00:00