Abstract：
:Tumor cells display different bioenergetic profiles when compared to normal cells. In the present work we showed metabolic reprogramming by means of inhibitors of histone deacetylase (HDACis), sodium butyrate and trichostatin A in breast cancer cells representing different stages of aggressiveness and metabolic profile. When testing the effect of NaB and TSA on viability of cells, it was shown that non-tumorigenic MCF-10A cells were less affected by increasing doses of the drugs than the tumorigenic, hormone dependent, tightly cohesive MCF-7, T-47D and the highly metastatic triple-negative MDA-MB 231 cells. T-47D cells were the most sensitive to treatment with both, NaB and TSA. Experiments measuring anchorage- independent growth of tumor cells showed that MCF-7, T-47D, and MDA-MB-231 cells were equally sensitive to the treatment with NaB. The NaB induced an attenuation of glycolysis, reflected by a decrease in lactate release in MCF-7 and T47D lines. Pyruvate kinase activity was significantly enhanced by NaB in MDA-MB-231 cells only. In contrast, the inhibitor enhanced lactate dehydrogenase activity specifically in T-47 D cells. Glucose-6-phosphate dehydrogenase activity was shown to be differentially modulated by NaB in the cell lines investigated: the enzyme was inhibited in MCF-7 cells, whereas in T-47D and MDA-MB-231 cells, G6PDH was activated. NaB and TSA were able to significantly increase the oxygen consumption by MDA-MB-231 and T-47D cells. Collectively the results show that epigenetic changes associated to acetylation of proteins in general affect the energy metabolism in all cancer cell lines and that mitochondria may occupy a central role in metastasis.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Rodrigues MF,Carvalho É,Pezzuto P,Rumjanek FD,Amoêdo NDdoi
10.1002/jcb.25036subject
Has Abstractpub_date
2015-05-01 00:00:00pages
797-808issue
5eissn
0730-2312issn
1097-4644journal_volume
116pub_type
杂志文章abstract：:Nuclear receptors (NRs) are key regulators of cell function and differentiation. We examined NR expression during osteogenic versus adipogenic differentiation of primary mouse calvarial osteoblasts (MOBs). MOBs were cultured for 21 days in osteogenic or adipogenic differentiation media. von Kossa and Oil Red O stainin...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21931
更新日期：2008-12-01 00:00:00
abstract：:The present study aimed to investigate the long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) involved in the progression of gallbladder cancer and explore the potential physiopathologic mechanisms of gallbladder cancer in terms of competing endogenous RNAs (ceRNAs). The original lncRNA and mRNA expression profi...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28953
更新日期：2019-12-01 00:00:00
abstract：:Membrane receptors for steroid hormones affect signaling pathways that modulate nuclear function, influence neuronal activity, ion flow, and the circulatory system. Indeed, 'new' steroid hormones have been identified by their interaction with membrane-initiated signaling systems. A brief summary of the FASEB Summer Re...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.10409
更新日期：2003-02-15 00:00:00
abstract：:A wide variety of environmental stimuli induce the expression of stress response genes, including high temperatures, hypoxia, heavy metal ions, and amino acid analogs. Stress genes are also induced by low frequency magnetic fields. The cellular response to magnetic fields is activated by unusually weak stimuli, and in...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/(sici)1097-4644(19991201)75:3<369::aid-jcb
更新日期：1999-12-01 00:00:00
abstract：:Adipose tissue is critical in obesity and type II diabetes. Blocking of adipocyte differentiation is one of the anti-obesity strategies targeting on strong rise in fat storage and secretion of adipokine(s). However, the molecular basis of adipocyte differentiation and its regulation remains obscure. Therefore, we expo...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.23223
更新日期：2011-10-01 00:00:00
abstract：:Inflammation, elicited in the skin following tissue damage or pathogen invasion, may become chronic with deleterious consequences. Tumor necrosis factor (TNF) is a key mediator of cutaneous inflammation and the keratinocyte an important protagonist of skin immunity. Calcitriol, the hormonally active vitamin D metaboli...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21650
更新日期：2008-05-15 00:00:00
abstract：:Interleukin-1 beta-converting enzyme (ICE) is a cysteine protease responsible for proteolytic activation of the biologically inactive interleukin-1 beta precursor to the proinflammatory cytokine. ICE and homologous proteases also appear to mediate intracellular protein degradation during programmed cell death. Inhibit...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：1997-01-01 00:00:00
abstract：:Pristimerin is a natural product derived from the Celastraceae and Hippocrateaceae families that were used as folk medicines for anti inflammation in ancient times. Although it has been shown that pristimerin induces apoptosis in breast cancer cells, the involved mechanism of action is unknown. The purpose of the curr...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21399
更新日期：2008-01-01 00:00:00
abstract：:The P-glycoprotein (p170, P-gp) encoded by human MDR1 gene functions as a pump to extrude anticancer drugs from cancer cells. Over-expression of p170 is closely related to primary and induced drug resistance phenotype of tumor cells. Recent studies have demonstrated that expression of cyclooxygenase-2 (COX-2) is posit...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22239
更新日期：2009-09-01 00:00:00
abstract：:Accumulating studies indicates that circular RNAs (circRNAs) play an imperative role in modulating cancer progression and metastasis. In the previous study, elevated circ_0029426 was first observed in glioblastoma (GBM) tissues compared with normal tissues by circRNA microarray. Our aim is to study the function and me...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28313
更新日期：2019-06-01 00:00:00
abstract：:Long noncoding RNAs (lncRNAs) areinvolvedin a variety of biological processes. In silkworm, numerous lncRNAs have been predicted through deep transcriptome sequencing, but no functional role has been experimentally validated yet. Here, we characterized a new lncRNA iab-1 that was mainly encoded by the intergenic regio...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28990
更新日期：2019-10-01 00:00:00
abstract：:Induction of definitive endoderm (DE) cells is a prerequisite for the whole process of embryonic stem (ES) cells differentiating into hepatic or pancreatic progenitor cells. We have established an efficient method to induce mouse ES cell-derived DE cells in suspension embryonic body (EB) culture. Similar to previous s...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22962
更新日期：2011-04-01 00:00:00
abstract：:Recycled iron from reticuloendothelial macrophages to erythroid precursors is important to maintain the iron homeostasis. However, the molecular mechanisms underlying iron homeostasis in macrophages are poorly understood. In this study, male Sprague-Dawley rats were treated with recombinant human erythropoietin (rHuEp...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21654
更新日期：2008-05-15 00:00:00
abstract：:Strong activation of the ERK signal is required for hepatocyte growth factor (HGF) to inhibit proliferation of the human hepatocellular carcinoma cell line HepG2. However, it is still to be elucidated whether the activation alone is sufficient to induce the inhibitory effect. In this study, we constructed HepG2 cell c...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21614
更新日期：2008-05-01 00:00:00
abstract：:Arsenic is the first metal to be identified as a human carcinogen. Arsenite, one inorganic form of arsenic, has been found to induce sister chromatid exchange, chromosome aberrations, and gene amplification in a variety of in vitro systems. In this study of arsenite-induced genotoxicity represented a micronuclei produ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：:The cholesterol hydroxylase/lyase (CHL) system, consisting of cytochrome P450scc, adrenodoxin (Adx) and adrenodoxin reductase (AdR), initiates mammalian steroidogenesis, converting cholesterol to pregnenolone. The foot-and-mouth disease virus 2A-based method allows to express multiple proteins from a single transcript...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.27577
更新日期：2019-03-01 00:00:00
abstract：:Beta-dystroglycan (beta-DG) is a widely expressed transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton, and thereby contributing to plasma membrane integrity and signal transduction. We previously observed nuclear localization of beta-DG in cultured cell lines, im...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22581
更新日期：2010-06-01 00:00:00
abstract：:Formyl peptide receptors (FPRs) are a family of classical chemoattractant receptors. Although FPRs are mainly expressed in phagocytic innate immune cells including monocytes/macrophages and neutrophils, recent reports demonstrated that additional different cell types such as T-lymphocytes and several non-immune cells ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.25877
更新日期：2017-06-01 00:00:00
abstract：:Garlic is known as a potent spice and a medicine with broad therapeutic properties ranging from antibacterial to anticancer, antidiabetic, and anticoagulant. Two major proteins of 40 KD and 14 KD constituting approximately 96% of total garlic proteins have been recently purified at our Institute. This immunocytochemic...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240580411
更新日期：1995-08-01 00:00:00
abstract：:Integrins are heterodimeric transmembrane cellular receptors that link the cell to its underlying substratum. Alterations in integrin expression and signaling have been implicated in many aspects of tumorigenesis and metastasis including cell survival, migration, and invasion. In prostate cancer, the progression from ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.10665
更新日期：2004-01-01 00:00:00
abstract：:In the present study, monoclonal antibodies were produced using porcine adipocyte extracts as the immunogen. Two of the monoclonal antibodies, designated CB6 and IB4, exhibited reactivity toward only cells containing lipid in stromal-vascular cell cultures. The antigens recognized by the CB6 and IB4 monoclonal antibod...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240450311
更新日期：1991-03-01 00:00:00
abstract：:Evidence indicates that agonists of neuronal nicotinic receptors (nAChRs), including nicotine, can induce neuroprotective and anti-apoptotic effects in the CNS. To study these mechanisms, the present study focused on nicotine-mediated modulation of the extracellular regulated kinase 1 and 2 (ERK1/2) pathway in culture...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21013
更新日期：2007-02-01 00:00:00
abstract：:Matrix metalloproteinases (MMPs), a family of zinc-dependent endopeptidases, are involved in a variety of physiological and pathological processes. We analyzed 11 data sets from Gene Expression Omnibus Database and found that MMP7 and MMP15 were highly expressed in multiple carcinomas. GSE13204 showed that MMP7 and MM...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.28351
更新日期：2019-06-01 00:00:00
abstract：:Previously, we reported that anti-apoptotic Bfl-1 is converted to a pro-apoptotic protein following fusion at its N-terminus with green fluorescent protein (GFP) (GFP-Bfl-1). In this study, we performed a Bfl-1 deletion study in order to elucidate the underlying mechanism of GFP-Bfl-1-induced cell death. We found that...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.20381
更新日期：2005-04-15 00:00:00
abstract：:We have partially purified and characterized an 87 kDa gelatinase activity expressed in later stage sea urchin embryos. Cleavage activity was specific for gelatin and no cleavage of sea urchin peristome type I collagen, bovine serum albumin or casein was detected. Magnesium and Zn2+ inhibited the gelatinase and Ca2+ p...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:During embryonic development, the hematopoietic system is the first to generate terminally differentiated, functional cell types. The urgent necessity for the early formation of blood and blood vessels during embryogenesis means that the induction, expansion, and maturation of these systems must be rapidly and precise...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.20191
更新日期：2004-10-01 00:00:00
abstract：:Transcription factor Sp-1 is an important fibrogenic factor that is involved in the pathogenesis of diabetic nephropathy. In this study, we examined the effect of Sp1 decoy oligodeoxynucleotides (ODNs) on the extracellular matrix (ECM) gene expression in cultured rat mesangial cells (RMC) and streptozotocin (STZ)-indu...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21440
更新日期：2008-02-01 00:00:00
abstract：:Hepatocytes can be generated from embryonic stem cells (ESCs) using inducers such as chemical compounds and cytokines, but issues related to low differentiation efficiencies remain to be resolved. Recent work has shown that overexpression of lineage-specific transcription factors can directly cause cells phenotypic ch...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.24604
更新日期：2013-11-01 00:00:00
abstract：:We have a long-standing interest in a nuclear protease which appears to be involved in carcinogenesis. We recently identified the protease as high temperature requirement factor A 1 (HtrA1), also known as Prss11, which is member of an oxidative stress-response family of proteases. HtrA1 has been classified as a secret...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.21804
更新日期：2008-09-01 00:00:00
abstract：:Previously, we have reported that inhibition of cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channels by glibenclamide induced intracellular Ca2+ release from IP(3)-sensitive stores and apoptosis in HepG2 human hepatoblastoma cells (Kim JA, Kang YS, Lee SH, Lee EH, Yoo BH, Lee YS. 1999. Biochem Bioph...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/1097-4644(20010401)81:1<93::aid-jcb1026>3.
更新日期：2001-01-01 00:00:00
