Abstract:
:Intracellular foreign protein (beta-galactosidase) expression in recombinant CHO cell lines in continuous culture was analyzed by developing a mathematical model that includes the effects of metabolic burden and cell cycle dependence of intracellular foreign protein expression. This combined growth kinetic and cell cycle model, assuming S- or G1-phase-dependent expression, was stimulated to predict productivity on a single-cell and culture-volume basis in continuous cultures. In the case of S-phase-dependent expression, the intracellular foreign protein level increases monotonically, but in the case of G1-phase-dependent expression it decreases monotonically with increasing dilution rate. Also, the trends of foreign protein concentration in the culture volume differ significantly between S- and G1-dependent expression kinetics. Thus, the cell cycle dependency of foreign protein expression should be included in process optimization concepts and operating strategies of continuous bioreactors.
journal_name
Ann N Y Acad Scijournal_title
Annals of the New York Academy of Sciencesauthors
Gu MB,Todd P,Kompala DSdoi
10.1111/j.1749-6632.1994.tb47392.xsubject
Has Abstractpub_date
1994-05-02 00:00:00pages
194-207eissn
0077-8923issn
1749-6632journal_volume
721pub_type
杂志文章abstract::This review will focus on the free radical signaling mechanism of preconditioning. The results from our laboratory as well as studies from other laboratories suggest that reactive oxygen species function as second messenger during myocardial adaptation to ischemia. This review provides evidence for the first time that...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
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更新日期:1999-06-30 00:00:00
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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abstract::Methamphetamine (METH) is a widely used "club drug" that produces neural damage in the brain, including the loss of some neurons. METH-induced striatal neuronal loss has been attenuated by pretreatment with the neurokinin-1 receptor antagonist WIN-51,708 in mice. Using a histologic method, we have observed the interna...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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journal_title:Annals of the New York Academy of Sciences
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
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更新日期:1988-01-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
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更新日期:2004-06-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1988.tb50859.x
更新日期:1988-01-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
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更新日期:1993-06-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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更新日期:1997-09-20 00:00:00
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journal_title:Annals of the New York Academy of Sciences
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abstract::Clinical and basic research in the area of drugs of abuse are of utmost importance since they provide the necessary background for health programs in one of the main problems of contemporary society. The available data in this field demonstrate that acute, subacute and/or chronic abuse of illicit drugs, e.g., cocaine,...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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更新日期:1996-10-31 00:00:00
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
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更新日期:2015-11-01 00:00:00
abstract::The search for tumor markers suitable for use in diagnosis and management of patients with lung cancer has met with limited success because each available marker lacks sufficient sensitivity and specificity. We have begun a collaborative study to evaluate multiple simultaneous serum markers to determine whether there ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1983.tb32885.x
更新日期:1983-01-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1996.tb34404.x
更新日期:1996-01-17 00:00:00
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journal_title:Annals of the New York Academy of Sciences
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abstract::This article reviews research on the Finnish model of peer-group mentoring (PGM). The theoretical foundation of the model is based on the constructivist theory of learning, the concept of autonomy in teaching profession, peer learning, and narrative identity work. The model has been disseminated nationwide in the educ...
journal_title:Annals of the New York Academy of Sciences
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abstract::Infection by different Leishmania spp. in cats has been reported in many countries. In Spain, since the first Leishmania infection described in 1933, sporadic clinical cases in cats have been reported. Various serologic studies performed in other areas of Spain have shown seroprevalences ranging between 1.7 and 60%. T...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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更新日期:1998-11-30 00:00:00
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journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
abstract::Questions about music's evolution and functions have long excited interest among scholars. More recent theoretical accounts have stressed the importance of music's social origins and functions. Autism and Williams syndrome, neurodevelopmental disorders supposedly characterized by contrasting social and musical phenoty...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
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更新日期:2009-07-01 00:00:00
abstract::Whole and nonadherent Peyer's patch cells were shown to present antigen to cloned antigen specific T-cells, albeit less efficiently than spleen cells. Unlike spleen cells, adherent PP cells did not present antigen, and PP lacked cells with classical dendritic morphology. The antigen presenting and MLR-stimulating cell...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.1983.tb26865.x
更新日期:1983-06-30 00:00:00
abstract::Externally guided sensory-motor processes deteriorate with increasing age. Internally guided, for example, predictive, behavior usually helps to overcome sensory-motor delays. We studied whether predictive components of visuomotor transformation decline with age. We investigated smooth pursuit eye movements (SPEM) of ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.2011.06114.x
更新日期:2011-09-01 00:00:00
abstract::Parathyroid hormone-related protein (PTHrP) participates in the regulation of endochondral bone development. After the cartilage mold is established in fetal life, perichondrial cells and chondrocytes at the ends of the mold synthesize PTHrP. This ligand then acts on PTH/PTHrP receptors on chondrocytes. As chondrocyte...
journal_title:Annals of the New York Academy of Sciences
pub_type: 社论,评审
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更新日期:2006-04-01 00:00:00
abstract::Aging is the major risk factor for both the development of chronic diseases and loss of functional capacity. Geroscience provides links among the biology of aging, the biology of disease, and the physiology of frailty, three fields where enormous progress has been made in the last few decades. While, previously, the f...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/nyas.13297
更新日期:2016-12-01 00:00:00
abstract::Washington State's law for sexually violent predators was enacted in 1990; since then, 14 other states have passed similar laws authorizing civil commitment for dangerous sexual offenders following their prison terms. Although the law has survived constitutional challenges at both the state and in the U.S. Supreme Cou...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章
doi:10.1111/j.1749-6632.2003.tb07327.x
更新日期:2003-06-01 00:00:00
abstract::Two types of mixed matrix membranes were developed by UOP in the late 1980s. The first type includes adsorbent polymers, such as silicalite-cellulose acetate (CA), NaX-CA, and AgX-CA mixed matrix membranes. The silicalite-CA has a CO(2)/H(2) selectivity of 5.15 +/- 2.2. In contrast, the CA membrane has a CO(2)/H(2) se...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1111/j.1749-6632.2003.tb06012.x
更新日期:2003-03-01 00:00:00
abstract::Gluten sensitivity is an autoimmune disease that usually causes intestinal atrophy resulting in a malabsorption syndrome known as celiac disease. However, gluten sensitivity may involve several organs and is often associated with extraintestinal manifestations. Typically, patients with celiac disease have circulating ...
journal_title:Annals of the New York Academy of Sciences
pub_type: 杂志文章,评审
doi:10.1196/annals.1381.034
更新日期:2007-06-01 00:00:00