Abstract:
:Isolated motor endplates from mouse intercostal muscles can be obtained after subcellular fractionation. On these motor endplates, localization of the nicotinic receptor and of the voltage-dependent Na+ channel coincides as demonstrated by double labeling with rhodamine alpha-bungarotoxin and a specific anti-Na+ channel monoclonal antibody. High density of Na+ channel at the motor endplate is confirmed by the enrichment in TTX binding sites as compared to the crude homogenate. In contrast isolated motor endplates are almost completely devoid of Ca2+ channel antagonist binding sites.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Dreyfus P,Rieger F,Murawsky M,Garcia L,Lombet A,Fosset M,Pauron D,Barhanin J,Lazdunski Mdoi
10.1016/s0006-291x(86)80098-5subject
Has Abstractpub_date
1986-08-29 00:00:00pages
196-201issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(86)80098-5journal_volume
139pub_type
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