Abstract:
:Kinin release and its involvement in inflammatory exudation were assessed by immuno-blot analysis of the kinin-precursor protein, high molecular weight kininogen (HK). HK consists of heavy (H) chain, bradykinin and light (L) chain. After bradykinin was released by plasma kallikrein, HK remains two-chain-kinin-free form;, i.e., H-chain and L-chain link each other through a disulfide bond. By Western blot analysis using antibody recognizing the light chain of HK, a band of 110-kD mass, which corresponds to intact HK, was detected in plasma after SDS-PAGE under reducing conditions, while a 46-kD band, corresponding to the light chain of HK, but no 110-kD band, was found in the exudate of rats with carrageenin-induced pleurisy at 3 hr as well as at 16 h. This result indicates that in the exudate most all of the HK molecules had released kinin to form kinin-free-HK, whereas the HK in the plasma remained intact. On the contrary, low molecular weight kininogen (LK) in the exudate was mostly in its intact form. These results indicate that plasma kallikrein could be activated in the exudate to release kinin from HK, as it reacts exclusively with HK and not with LK, and they are also mostly consistent with the features of the kinin release from the exudate and the plasma. That is, no kinin was detected in the exudate when the latter was incubated with plasma kallikrein, whereas salivary kallikrein did release kinin, indicating that kinin had already been released from HK, but not from LK in the exudate. Immunoblot analysis of HK in the pleural exudate also demonstrated no kinin involvement in phorbol myristate acetate- or zymosan-induced pleurisy, since no light chain band, but an intact HK band, was found in the exudates from these pleurisies.
journal_name
Life Scijournal_title
Life sciencesauthors
Fujie H,Hayashi I,Oh-ishi Sdoi
10.1016/0024-3205(93)90206-isubject
Has Abstractpub_date
1993-01-01 00:00:00pages
1691-701issue
22eissn
0024-3205issn
1879-0631pii
0024-3205(93)90206-Ijournal_volume
53pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::The aim of the present work was to study the effects of various durations of fasting (12, 24 and 72 hours) on brown adipose tissue (BAT) thermogenic parameters--cytrochrome-c-oxidase (COX) activity, GDP-binding activity and uncoupling protein (UCP1) content--and also on morphological features of different mitochondria...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00153-2
更新日期:1998-01-01 00:00:00
abstract::Increased levels of particulate matter (PM) air pollutants in East Asia have resulted in detrimental health impacts increasing morbidity and mortality. Epidemiological studies suggest a possible relation between the cutaneous exposure of PM and increased oxidative stress and inflammation which lead to skin lesions. Th...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116714
更新日期:2019-09-15 00:00:00
abstract::Several cryopreservation methods for precision-cut rat liver slices (PCLS) have been proposed, allowing a short-term (a few hours) maintainance of viability and functionality upon thawing. The aim of the present study was to test the metabolic capacity of PCLS cryopreserved by an ultrarapid method. The biotransformati...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01031-1
更新日期:2001-04-13 00:00:00
abstract:UNLABELLED:Based on preclinical findings, cellular therapy has become a promising therapeutic approach for neonatal hypoxia-ischemia (HI). However, before translation into the clinical setting, new and effective routes of cell delivery must be determined. Intra-arterial (IA) delivery is an attractive route of cellular ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2013.10.017
更新日期:2014-02-06 00:00:00
abstract:AIM:The purpose of this study was to investigate whether the flavonoid quercetin can prevent alterations in the behavioral tests and of cholinergic neurotransmission in rats submitted to the ethidium bromide (EB) experimental demyelination model during events of demyelination and remyelination. MAIN METHODS:Wistar rat...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.03.033
更新日期:2014-05-17 00:00:00
abstract::Besides an isomer of the cardenolide ouabain, a material with a similar HPLC retention time as ouabain but cross-reactivating with antibodies against the bufadienolide proscillaridin A and inhibiting the sodium pump is known to circulate in human blood plasma (B. SICH et al., Hypertension 27, 1073-1078 (1996).). The c...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00023-x
更新日期:1998-01-01 00:00:00
abstract::Based on the finding of decreased mitochondrial complex I activity in the substantia nigra of patients with Parkinson's disease, we propose that the consequent reduction of ATP synthesis and increased generation of reactive oxygen species may be a possible cause of nigrostriatal cell death. Since sulfhydryl groups are...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/s0024-3205(98)00472-x
更新日期:1999-01-01 00:00:00
abstract:AIMS:Asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase inhibitor, has been reported to be a novel marker for the progression of chronic kidney disease (CKD). We have recently found that accumulation of ADMA could trigger peritubular capillary loss, thus contributing to tubulointerstitial ischemia ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.03.018
更新日期:2009-06-05 00:00:00
abstract::Hyperketonemia has been identified as an important factor in diabetic pregnancy affecting growth and development of the offspring. In order to assess the immediate metabolic alterations in embryos caused by excess ketone bodies, we studied rat embryonic neural tissue exposed to a high concentration of beta-hydroxybuty...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)01110-7
更新日期:1998-01-01 00:00:00
abstract::Proteinaceous inhibitors with high inhibitory activities against human neutrophil elastase (HNE) were found in seeds of the Tamarind tree (Tamarindus indica). A serine proteinase inhibitor denoted PG50 was purified using ammonium sulphate and acetone precipitation followed by Sephacryl S-300 and Sephadex G-50 gel filt...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.10.053
更新日期:2005-05-06 00:00:00
abstract::Hydroxyethyl starch (HES) found in rat serum and spleen after single and daily administrations of 0.9 g/kg for 1 week was characterized by gas-liquid chromatography. There was very little difference in the degree of substitution (D.S.) and molar substitution (M.S.) of HES in serum samples obtained at 1 hour and 57 day...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90737-3
更新日期:1983-11-14 00:00:00
abstract::The ATP-binding cassette (ABC) and solute carrier (SLC) transporter families consist of common drug transporters that mediate the efflux and uptake of drugs, respectively, and play an important role in the absorption, distribution, metabolism and excretion of drugs in vivo. Rheumatoid arthritis (RA) is an autoimmune d...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.118907
更新日期:2021-01-08 00:00:00
abstract::We examined, in young adult women, the association between current low dose oral contraceptive (OC) use and plasma levels of C-reactive protein (CRP), an acute phase reactant predictive of cardiovascular disease risk. We conducted a cross-sectional analysis of 30 healthy, non-smoking, non-obese women (18 OC users and ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00425-9
更新日期:2003-07-25 00:00:00
abstract::CGS 10746B, a benzothiadiazepine, has a behavioral profile in mice and monkeys similar to the atypical antipsychotic clozapine. Unlike clozapine, CGS 10746B suppresses dopamine neuron firing rates and, when administered at behaviorally effective doses by the oral or intraperitoneal route, decreases neostriatal dopamin...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90017-2
更新日期:1986-08-25 00:00:00
abstract::Tifluadom, although structurally a 1,4 benzodiazepine, has no affinity for the 3H-flunitrazepam binding site, but is a potent displacer of 3H-bremazocine from its opioid binding site. Tifluadom is characterised as an opiate kappa-receptor agonist in vitro and in vivo with potent analgesic activity in animals and no de...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90346-0
更新日期:1982-09-20 00:00:00
abstract::The dramatic activities of bradykinin and related peptides as mediators of pain, inflammation and hypotension have been intensely studied for several decades. More recently, the involvement of bradykinin in regulation of ion transport by epithelia, hormone release from endocrine organs, energy metabolism, tissue growt...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(92)90201-y
更新日期:1992-01-01 00:00:00
abstract::Icariin (ICA), a flavonol glycoside isolated from Epimedium, has been considered as a potential alternative therapy for ischemic stroke. However, the protective mechanisms of ICA on cerebral ischemia-reperfusion (I/R) are not fully illuminated yet. The effects of ICA on ER stress and inflammatory response which were i...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117847
更新日期:2020-08-15 00:00:00
abstract::Involvement of T-type voltage dependent Ca2+ channels (VDCCs) on morphine antinociception, in the development of tolerance and dependence to morphine, and naloxone-precipitated abstinence syndrome in morphine dependent mice was examined by using mibefradil, a T-type VDCCs blocker. Mice were rendered tolerant and depen...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)01736-8
更新日期:2002-06-28 00:00:00
abstract::Chemokines constitute a large family of structurally-related small cytokines originally identified as factors regulating the migration of leukocytes in inflammatory and immune responses. Production of chemokines and their receptors in the brain has been reported under various pathological conditions. We revealed that ...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2003.09.019
更新日期:2003-12-05 00:00:00
abstract::Bradykinin receptor stimulation results in G-protein-coupled phospholipase activation, initiating protein kinase C (PKC) stimulation and cytosolic free Ca2+ concentration ([Ca2+]i) rises as signalling pathways. Using Rb+ as a tracer for K+, we have studied the mechanisms involved in bradykinin-stimulated Rb+ influx in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(96)00530-9
更新日期:1996-01-01 00:00:00
abstract::Sustained levels of leucine comparable to those of human Maple Syrup Urine Disease (MSUD) were achieved in blood and brain of rats by subcutaneous leucine administration twice a day from the 6th to the 28th day of life. Control rats were treated with saline in the same volumes. Behavioral studies using aversive and no...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00301-x
更新日期:1999-01-01 00:00:00
abstract::Resveratrol, a polyphenolic substance found in grape skin, is proposed to account in part for the protective effect of red wine in the cardiovascular system. The aim of the present study is to investigate the action and possible mechanisms of resveratrol-produced regulation of plasma glucose in normal and diabetic rat...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.02.002
更新日期:2007-04-10 00:00:00
abstract:AIMS:The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite. MATERIALS AND METHODS:Firstly, the biological activity of rhBMP-2 and rhCXCL13 rele...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116743
更新日期:2019-10-01 00:00:00
abstract::This paper demonstrates marked differences between blood levels and those in the CSF for three anterior pituitary peptide hormones, prolactin, luteinizing hormone (LH) and adrenocorticotrophin (ACTH) in the rhesus monkey. CSF levels of endogenous prolactin (measured by radioimmunoassay) are about 20% of those in the b...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90064-4
更新日期:1983-04-18 00:00:00
abstract::Psoriasis is an inflammatory skin disease with preference for the skin and joints that occurs due to hyper-proliferation and abnormal apoptosis of keratinocytes. DUSP1 expression in dermal mesenchymal stem cells (MSCs) is obviously lower in psoriasis patients than that in healthy individuals. The present study aimed t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.03.018
更新日期:2019-04-15 00:00:00
abstract:AIMS:In myocardial ischemia-reperfusion (MI/R) injury, impaired autophagy function worsens cardiomyocyte death. AMP-activated protein kinase (AMPK) is a heterotrimeric protein that plays an important role in cardioprotection and myocardial autophagic function. AMPKα1 and α2 are localized primarily in the cytoplasm and ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.04.002
更新日期:2019-05-15 00:00:00
abstract::The present study shows that morphine reduces the pulmonary inflammatory response to intranasal influenza virus infection in rats. Rats were infected with rat-adapted influenza virus (RAIV), which is a unique infectious agent because normal rats develop an acute pulmonary inflammatory response to RAIV and rapidly clea...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00348-3
更新日期:1999-01-01 00:00:00
abstract::Intestinal mucosal capsaicin-sensitive afferent nerves mediate, in part, the protective mesenteric hyperemia after intraduodenal acidification. Mechanisms associated the sensory neuropeptides, e.g. calcitonin gene-related peptide (CGRP), substance P, and ruthenium red-sensitive cation channels contribute to acid-induc...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)02106-9
更新日期:2002-10-18 00:00:00
abstract::Cordyceps sinensis (C. sinensis) is one of the well known fungi used in traditional Chinese medicine for treatment asthma and bronchial and lung inflammation. In this study, effects of C. sinensis methanolic extracts on bronchoalveolar lavage fluids (BALF) cells proliferation, inflammatory cytokines production, and ge...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)01011-0
更新日期:2001-01-19 00:00:00
abstract::Using absorption spectroscopy we have found that metallothionein I at concentrations as low as 1 microM reduces ferricytochrome c. This reduction is more potent than that of another abundant intracellular thiol, glutathione. Also, oxidation of ferricytochrome c which had been reduced by metallothionein I occurs more s...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90019-y
更新日期:1993-01-01 00:00:00