Myofibroblast-like cells produce mRNA for type I and III procollagens in chronic active hepatitis.

Abstract:

:In chronic active hepatitis the rate of collagen biosynthesis is largely determined by intracellular mRNA concentrations. To localize procollagen mRNA-producing cells, we investigated biopsy specimens from five patients with hepatitis B surface antigen-positive chronic active hepatitis and five patients without liver disease by in situ hybridization. We used type I and III procollagen cDNAs for transcription to (35S)-labeled probes. Parallel sections were stained with anti-actin monoclonal antibodies. Our results show that cells in which collagen synthesis is ostensibly enhanced can be localized by in situ hybridization of procollagen mRNAs. These cells were also anti-actin-positive in parallel sections and were localized in areas of inflammatory cell infiltration and necrosis. We conclude that myofibroblast-like cells may express procollagen mRNAs in chronic active hepatitis. Moreover, in situ hybridization may be a valuable diagnostic tool for providing additional morphologic information on the degree of fibrogenesis activity.

journal_name

Scand J Gastroenterol

authors

Högemann B,Gillessen A,Böcker W,Rauterberg J,Domschke W

doi

10.3109/00365529309096093

subject

Has Abstract

pub_date

1993-07-01 00:00:00

pages

591-4

issue

7

eissn

0036-5521

issn

1502-7708

journal_volume

28

pub_type

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