Abstract:
:Cognitive function of rats treated with saline (control), THA (8 mg/kg, i.p.), scopolamine (5 mg/kg, i.p.), or a combination of THA (8 mg/kg) and scopolamine (5 mg/kg) was tested in the Morris water maze. The latency to find the platform in the water maze was used to evaluate performance. THA did not significantly alter the latency period as compared to control rats. Scopolamine resulted in a highly significant (p<0.01) increase in latency period (183% increase) as compared to saline treated controls. However, when THA was concurrently administered with scopolamine, it was able to completely reverse the performance decrement induced by scopolamine. Immediately following spatial reference memory testing, animals were sacrificed by decapitation one hour post injection. Brains were immediately removed and the cortex, hippocampus, hypothalamus, and pituitary were dissected and their choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activity were determined spectrophotometrically. THA administration resulted in a significant increase in ChAT activity in the cortex (23% increase). However, when THA was concurrently administered with scopolamine, a significant increase in ChAT activity was observed in cortex (77% increase), hippocampus (32% increase), hypothalamus (97% increase), and pituitary (92.5% increase). THA administration resulted in a significant decrease in AChE activity (p<0.001) in cortex (62% decrease), hippocampus (78% decrease), and hypothalamus (90% decrease). When tacrine was administered with scopolamine, a significant increase was found in the cortex (197% increase) and the hippocampus (207% increase). In conclusion, the increase in ChAT activity produced by tacrine may in part explain its ability to reverse the scopolamine induced decrease in spatial reference memory and may play a role in its beneficial effect in improving cognitive ability.
journal_name
Life Scijournal_title
Life sciencesauthors
Jackson JJ,Soliman MRdoi
10.1016/0024-3205(95)02254-6subject
Has Abstractpub_date
1996-01-01 00:00:00pages
47-54issue
1eissn
0024-3205issn
1879-0631pii
0024320595022546journal_volume
58pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Using a ligation method, rat rectal epithelium was exposed to 2% sodium salicylate, and light and electron microscopic methods were used to assay for: 1) permeability of the epithelium to a marker dye, trypan blue, and 2) damage expressed in terms of disruption of the epithelial surface. Rectal mucosa was exposed to s...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90656-2
更新日期:1983-09-12 00:00:00
abstract::The aggregation of melanin-granules within fish pigment cells (melanophores) can be elicited either by electrical stimulation of intrinsic nerves or by the addition of adrenergic agonists. The pigment aggregation seems to be mediated by alpha-2-adrenoceptors. In this investigation we have used various agonists and ant...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90595-8
更新日期:1985-09-16 00:00:00
abstract::We had previously hypothesized that linoleic acid (LA) was essential for development of alcoholic induced liver injury in our rat model. Male Wistar rats were fed a nutritionally adequate diet (25% calories as fat) with ethanol (8-17 g/kg/day). The source of fat was tallow (0.7% LA), lard (2.5% LA) or tallow supplemen...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90599-7
更新日期:1989-01-01 00:00:00
abstract::We investigated the mechanism underlying the priming effect of TNF-alpha on fMLP-stimulated superoxide production in human neutrophils. TNF-alpha enhanced fMLP-stimulated superoxide production in a concentration-dependent manner. TNF-alpha also induced sphingomyelin (SM) hydrolysis and increased the formation of its m...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00587-1
更新日期:2000-01-01 00:00:00
abstract:AIMS:Circular RNAs (circRNAs) have been emerged as novel regulators in multiple tumorigenesis, including melanoma. CircRNA_0084043 was recently demonstrated to be deregulated in human melanoma cells. Nevertheless, its role and mechanism are largely unrevealed in melanoma. MATERIALS AND METHODS:Expression of circ_00840...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117323
更新日期:2020-02-15 00:00:00
abstract::Intracerebroventricular (i.c.v.) administration of thyrotropin-releasing hormone (TRH) in a range from 0.1 to 100 micrograms induced a dose-related increase in blood pressure in conscious rats, whereas TRH-free acid (TRH-OH) and histidyl-proline diketopiperazine (His-Pro-DKP), metabolites of TRH, did not. The blood pr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90586-8
更新日期:1987-03-30 00:00:00
abstract:AIM:Nuclear factor-kappa B inhibitor alpha (IκBα) polymorphisms were found to be associated with inflammatory diseases. However, the association between IκBα polymorphisms with gastric cancer is still unknown. We aim to investigate the association between IκBα polymorphisms and gastric cancer risk in a large population...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.02.016
更新日期:2011-04-25 00:00:00
abstract:HEADING AIMS:MicroRNA-27b (miR-27b) has been shown to play a role in the progression of many different forms of cancer, but its specific relevance in the context of non-small cell lung cancer (NSCLC) remains uncertain. As such, this study sought to explore the role of miR-27b in NSCLC and the mechanisms whereby it func...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.117238
更新日期:2020-08-01 00:00:00
abstract::The distribution of immunoreactive-dynorphin (ir-Dyn) in isolated subpopulations of bovine adrenal chromaffin cells was examined and compared with that of adrenaline (A), noradrenaline (NA) and ir-Leucine-Enkephalin (ir-Leu-Enk). Using a stepwise bovine serum albumin (BSA) gradient, various populations of catecholamin...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90042-5
更新日期:1983-01-17 00:00:00
abstract::The effects of exposure to a schedule of unpredictable cold-immobilization stress on voluntary ethanol consumption were examined. Following testing for ethanol preference, rats were divided into high, medium and low ethanol consuming groups on the basis of daily ethanol intake (g/kg/day) and exposed to immobilization ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90580-7
更新日期:1987-03-30 00:00:00
abstract::Isolated guinea-pig and rabbit airway smooth muscle preparations lacking cartilage are less able to contract, in response to methacholine, histamine and K+, in the absence of extracellular Ca2+ than cartilage-containing preparations removed from the same animal. Cartilage apparently provides utilizable Ca2+ for contra...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90563-1
更新日期:1986-04-21 00:00:00
abstract::The effects of C-reactive protein (CRP), the prototypical acute-phase reactant were studied on human polymorphonuclear leukocytes (PMNL) challenged with N-formyl-methionyl-leucyl-phenylalanine (fMLP) and platelet-activating factor (PAF). CRP at 8-64 micrograms/ml concentrations inhibited degranulation and superoxide p...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90613-9
更新日期:1989-01-01 00:00:00
abstract::In order to estimate the intestinal stability of ebiratide [H-Met(O2)-Glu-His-Phe-D-Lys-Phe-NH(CH2)8-NH2], a novel adrenocorticotropic hormone (ACTH) analogue, after oral administration, the hydrolytic properties of ebiratide were determined in the rat small intestinal fluid and mucosal homogenates. Ebiratide was extr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00674-1
更新日期:1994-01-01 00:00:00
abstract::The present investigation examined the interaction between fluoxetine enantiomers and LSD in rats trained with LSD as a discriminative stimulus. The dose response relationships for LSD alone and in combination with either (+)- or (-)-fluoxetine were determined. In both instances, the LSD dose response relationship was...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00490-0
更新日期:1996-01-01 00:00:00
abstract::The cellular electrophysiology of left ventricular preparations from guinea pig hearts was studied 1 hour, 24 hours, and 4-6 weeks after myocardial infarction produced by 6-8 single ties of the distal left coronary artery system or after sham operation. Microelectrode recordings were used to monitor cells from the end...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90343-1
更新日期:1985-06-24 00:00:00
abstract:AIMS:MicroRNA-1 (miR-1) has been demonstrated as a tumor-suppressive miRNA, which shows a down-regulated pattern in several human malignancies including hepatocellular carcinoma (HCC). However, the pathophysiologic roles of miR-1 and their mechanisms in HCC tumorigenesis are still not totally elucidated. MAIN METHODS:...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.08.015
更新日期:2012-10-05 00:00:00
abstract:AIMS:Metformin is an insulin sensitizing agent with beneficial effects in diabetic patients on glycemic levels and in the cardiovascular system. We examined whether the metabolic changes and the vascular dysfunction in monosodium glutamate-induced obese non-diabetic (MSG) rats might be improved by metformin. MAIN METH...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.11.005
更新日期:2012-01-30 00:00:00
abstract::Since reduced levels of brain serotonin are known to cause behavioural abnormalities, to which diabetics are also prone, we investigated the effect, in rats, of chronic diabetes on brain serotonin concentration and on the numbers of 5-HT(1A) and 5-HT2 receptors in cerebral cortex and brainstem. Our data show that stre...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00084-2
更新日期:1997-01-01 00:00:00
abstract::Mucosal mast cells are implicated in visceral hypersensitivity associated with irritable bowel syndrome (IBS). In this study, we investigated the role of mast cells in the development of visceral hypersensitivity by using mast cell deficient (Ws/Ws) rats and their control (W+/W+). In W+/W+ rats, an injection of 2,4,6-...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.11.027
更新日期:2008-02-13 00:00:00
abstract::Oxidative stress (free radical generation exceeds antioxidant defense) occurs in many diseases and after exposure to certain chemicals. We automated assays estimating the antioxidant status of serum and lipid hydroperoxide concentrations using the Syva-30R automated analyzer. In this assay, the antioxidant status of s...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00401-3
更新日期:1997-01-01 00:00:00
abstract::Four Rhesus monkeys were subjected to daily administration of 2.5 mg of tetrahydrocannabinol (THC)/kg body wt, after establishing the norms for complete blood count, T- and B-cell concentrations, and the dose response of thymidine incorporation after PHA stimulation. THC was administered daily for 3 weeks, the treatme...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90642-7
更新日期:1982-01-04 00:00:00
abstract::A simple procedure has been developed for the large scale purification of fetal bovine serum acetylcholinesterase (AChE) (EC 3.1.1.7). The procedure involves two steps: batch adsorption of the AChE from 250 L of serum onto a procainamide affinity Sepharose 4B gel; and analytical procainamide affinity chromatography of...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90530-8
更新日期:1986-07-21 00:00:00
abstract:AIMS:Hyperthermia is a characteristic functional effect of sleep deprivation (SD). We hypothesize here that prostaglandin E2 (PGE2) could be involved in hyperthermia induced by sleep deprivation. MAIN METHODS:To address this issue we examined the effects of a selective cyclo-oxygenase-2 inhibitor (COX-2) agent on hype...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.12.009
更新日期:2009-02-27 00:00:00
abstract::The biosynthesis of phospholipids during differentiation of normal and dystrophic muscle cells was studied using [32Pi] added to primary culture media. Labeled phosphatides were separated by two-dimensional thin-layer chromatography, exposing the plates to HCl vapors between solvents to cleave the alk-1-enyl bonds. Ph...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90006-8
更新日期:1986-01-13 00:00:00
abstract::Adenylate cyclase activity in pancreatic acinar cell membranes was determined in rats that had undergone a treatment with pentagastrin (250 micrograms/kg, intraperitoneal three times daily) for 1 week or that had undergone small bowel resection (90%) and were sacrified at 2 weeks, 1 month and 6 months after interventi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02226-9
更新日期:1995-01-01 00:00:00
abstract::Synthetic human pancreatic Growth Hormone-Releasing Factor (hpGRF) elevated the plasma concentration of growth hormone (GH) in young and adult domestic fowl. This in vivo effect of hpGRF appeared to be largely similar for both the 32 amino-acid (hpGRF 1-32) or 40 amino-acid (hpGRF 1-40) polypeptide, although the effec...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90083-3
更新日期:1984-03-19 00:00:00
abstract:AIMS:Dietary habits are crucial factors affecting metabolic homeostasis. However, few animal experiments have addressed the effects of long-term feeding with soft food on parameters reflecting systemic health. MAIN METHODS:Using mice, we compared the effects of short (3 days) and long (17 weeks from weaning) feeding p...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.03.022
更新日期:2014-05-08 00:00:00
abstract::Hypoxic preconditioning (HPC) has been well demonstrated to have potent protective effects in many cell types; however, the mechanisms responsible for this phenomenon are not fully understood. Recently, glucose-regulated protein 78 (GRP78), an inducible molecular chaperon, was indicated to be associated with ischemic ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.08.015
更新日期:2007-09-08 00:00:00
abstract::Decreased sweat secretion is a primary side effect of topiramate in pediatric patients, but the mechanism underlying this effect remains unclear. This study aimed to better understand how topiramate decreases sweat secretion by examining its effect on the expression of carbonic anhydrase (CA) II and aquaporin-5 (AQP5)...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.04.018
更新日期:2007-06-06 00:00:00
abstract::[3H]A-69024 has been prepared as a radioligand for studying the dopamine D1 receptor. [3H]A-69024 binds to rat striatal membranes with a KD = 14.3 +/- 3.2 nM (mean +/- SEM; n = 3) and Bmax = 63.5 +/- 12.8 fmol/mg wet tissue (1.8 +/- 0.3 pmol/mg protein). This ligand binds to only one site with a Hill coefficient close...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02212-2
更新日期:1995-01-01 00:00:00