Abstract：
:Application of mesenchymal stromal cells (MSCs) has been proposed for solid organ transplantation based on their potent immuno-modulatory effects in vitro and in vivo. We investigated the potential of MSCs to improve acceptance of kidney transplants in an MHC-incompatible rat model including isogeneic kidney transplantation (RTx) as control. MSCs were administered i.v. or i.a. at time of transplantation. No immunosuppression was applied. Renal function was monitored by serum-creatinine, histopathology, immunochemistry for graft infiltrating cells and expressions of inflammatory genes. We demonstrated the short-term beneficial effects of MSC injection. In the long term, however, MSC-related life-threatening/shortening events (thrombotic microangiopathy, infarctions, infections) were evident despite decreased T- and B-cell infiltration, lower interstitial inflammation and downregulated inflammatory genes particularly after i.a. MSC injection. We conclude that i.a. MSC administration provides efficient immunomodulation after allogeneic RTx, although timing and co-treatment strategies need further fine-tuning to develop the full potential of powerful cell therapy in solid organ transplantation.
journal_name
Transpl Immunoljournal_title
Transplant immunologyauthors
Koch M,Lehnhardt A,Hu X,Brunswig-Spickenheier B,Stolk M,Bröcker V,Noriega M,Seifert M,Lange Cdoi
10.1016/j.trim.2013.08.004subject
Has Abstractpub_date
2013-12-01 00:00:00pages
43-50issue
1-4eissn
0966-3274issn
1878-5492pii
S0966-3274(13)00068-3journal_volume
29pub_type
杂志文章abstract：BACKGROUND:Brain death impairs donor organ quality and accelerates immune responses after transplantation. Detailed aspects of immune activation following brain death remain unclear. We have established a mouse model and investigated the immediate consequences of brain death and anesthesia on immune responses. METHODS...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2012.04.002
更新日期：2012-08-01 00:00:00
abstract：:Induction of donor-specific hyporesponsiveness would minimize the need for intensive immunosuppression in the clinical setting of graft rejection and dendritic cells (DCs) might be useful tools for this purpose. Besides their ability to induce immunogenic T-cell responses, these antigen presenting cells can lead to T-...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2006.03.001
更新日期：2006-06-01 00:00:00
abstract：:The induction of antigen-specific T cell tolerance and its maintenance in the periphery are critical for the immune system to prevent autoaggressive immune responses. Our current state of knowledge about the immunoregulatory mechanisms responsible for T cell tolerance in the periphery offers new possibilities for immu...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/S0966-3274(03)00057-1
更新日期：2003-07-01 00:00:00
abstract：:Limiting dilution analysis technique was used to enumerate the circulating precursor frequency of donor and third-party-reactive helper T lymphocytes (HTLpf) in 28 renal allograft recipients before (pre-tx) and at three intervals (T1: 60-90 days, T2: 120-180 days, T3: 360-1620 days) after transplantation (post-tx). Tw...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(97)80048-2
更新日期：1997-06-01 00:00:00
abstract：BACKGROUND:We recently reported that autoreactive antibody (Ab) against nuclear histone H1 had been identified as an immunosuppressive factor in a rat tolerogenic orthotopic liver transplantation (OLT) model. The present study aimed to determine whether the up-regulation of antihistone H1 Ab by histone H1 vaccination l...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2007.01.003
更新日期：2007-04-01 00:00:00
abstract：:Calcineurin a calmodulin-dependent phosphatase plays a critical role in calcium-dependent activation of T-lymphocytes and is the major target for the inhibitory actions of the immunosuppressive drugs Tacrolimus (FK506) and Cyclosporin A (CsA). Calcineurin is a dimeric protein consisting of distinct A (catalytic) and B...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(00)00020-4
更新日期：2000-06-01 00:00:00
abstract：BACKGROUND:After lung transplantation, the major complication limiting the long-term survival of allografts is obliterative bronchiolitis (OB), characterized by chronic rejection. Innate immune responses contribute to the development of OB. In this study, we used a murine heterotopic tracheal transplantation mouse mode...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2018.11.006
更新日期：2019-04-01 00:00:00
abstract：BACKGROUND:Although high-mobility group box-1 (HMGB1), which is a nuclear protein, was reported to enhance the allogeneic responses in transplantation, the effect of HMGB1 on bronchiolitis obliterans syndrome (BOS) is unknown. METHODS:A murine heterotopic tracheal transplantation model was used. Protein concentrations...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2018.11.007
更新日期：2019-04-01 00:00:00
abstract：:TCR-beta (T cell receptor-beta chain) transgenic mice have altered lymphocyte development. TCR-beta transgenic mice are hyporesponsive to alloantigens in vivo and are deficient in gamma delta T cells. In order to begin a study of the relationship between a deficiency of alloreactive gamma delta cells and the defective...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/0966-3274(94)90063-9
更新日期：1994-09-01 00:00:00
abstract：:Human anti-pig antibodies were obtained by perfusing pig hearts (n = 4) and kidneys (n = 8) with human AB or O plasma followed by elution with 3 M NaSCN. The antibodies were screened against a panel of 132 synthetic carbohydrates conjugated to bovine serum albumin using an enzyme-linked immunoassay. An anti-immunoglob...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/0966-3274(93)90047-c
更新日期：1993-01-01 00:00:00
abstract：:Previously, we established a murine model, that involves the engraftment of fully allogeneic T cell depleted donor bone marrow cells in sublethally irradiated and single dose anti-CD3 treated recipient mice. These mice developed permanent stable multilineage mixed chimerism and donor-specific tolerance without graft-v...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/S0966-3274(02)00157-0
更新日期：2003-07-01 00:00:00
abstract：BACKGROUND:Donor passenger leukocytes (DPLs) that migrate after organ transplantation stimulate the recipient immune system and normally cause rejection and graft vs. host reaction. However, DPLs also contribute to the unresponsiveness to the donor organ. The quantity and quality of these migrating cells are considered...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2003.10.001
更新日期：2004-01-01 00:00:00
abstract：:Transplant rejection is a multifactorial process involving complex interactions between components of the innate and the acquired immune system. In view of the shortage of donor organs available for transplantation, xenotransplantation of pig organs into man has been considered as a potential solution. However, in com...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/s0966-3274(02)00042-4
更新日期：2002-05-01 00:00:00
abstract：:Autoimmune diseases are the result of an interplay between predisposing genes and triggering environmental factors, leading to loss of self-tolerance and an immune-mediated destruction of autologous cells and/or tissues. Genes in the HLA complex are among the strongest predisposing genetic factors. The HLA complex gen...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/j.trim.2005.03.021
更新日期：2005-08-01 00:00:00
abstract：:Treatment with autologous bone marrow transplantation (ABMT) can change the natural history of diabetes in patients with new-onset Type 1 diabetes (T1D). Effects of syngeneic bone marrow transplantation (syn-BMT) on diabetic nephropathy were studied in streptozotocin-induced diabetic mice. Diabetic mice received sibli...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2009.12.003
更新日期：2010-02-01 00:00:00
abstract：BACKGROUND:Xenotransplantation is an appealing alternative to human allotransplantation because of a worldwide shortage of organs. One of the obstacles for xenografts is cellular rejection by the innate immune system, comprised of NK cells, monocytes, and macrophages. In this study the inhibitory function of HLA-G1, a ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2014.12.004
更新日期：2015-03-01 00:00:00
abstract：:We present management strategies utilised for the first case of an urgent live-donor ABO incompatible B blood group renal transplant, in a patient with a prior A blood group lung transplant for cystic fibrosis. Three years on, renal function is excellent and stable, whilst lung function has improved. ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2016.09.002
更新日期：2016-11-01 00:00:00
abstract：:Recently, the signal transducer and activator of transcription 4 (STAT4) gene have been associated with multiple autoimmune diseases. Taking into consideration that the different autoimmune diseases may share some common pathogenetic pathways, the aim of the present study was to evaluate the role of STAT4 rs7574865 po...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2011.01.001
更新日期：2011-05-01 00:00:00
abstract：BACKGROUND:Conditioning chemotherapies for hematopoietic stem cell transplantation (HSCT), especially those that include total body irradiation, can result in serious complications such as graft-versus-host disease (GVHD). Human leukocyte antigen G (HLA-G) is a non-classical class I molecule with multiple immunoregulat...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2018.12.001
更新日期：2019-04-01 00:00:00
abstract：:Rituximab is frequently used in the setting of ABO-incompatible renal transplants, and highly sensitized patients. Its interference with B-cell flow cytometric crossmatch (B-FCXM) is well known. However, its effect on the T-cell flow cytometric crossmatch (T-FCXM) has not been described. We aimed to evaluate the effec...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2020.101360
更新日期：2021-02-01 00:00:00
abstract：:Immunological monitoring of transplant recipients is an attractive concept. Cytokines provide an obvious focus for research, as they are central to the human immune response. This study aimed to identify cytokines whose sequential gene expression differentiated rejectors from non-rejectors immediately following renal ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2005.02.003
更新日期：2005-06-01 00:00:00
abstract：BACKGROUND:So far, the ImmuKnow Immune Cell Function Assay (Cylex, Inc., Columbia, MD, USA) has been used to assess risks of infection and rejection in transplant patients. We hypothesized that the ImmuKnow assay might be used for mortality screening in transplant patients overall. METHODS:In the period of February 20...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2010.12.005
更新日期：2011-05-01 00:00:00
abstract：:Although natural killer cells lyse targets without pre-sensitization, and in an MHC-unrestricted manner, they can also respond to healthy allogeneic cells of different MHC type. Such alloreactivity is a consequence of NK cells using clonally distributed, inhibitory MHC class I receptors to achieve tolerance to healthy...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/j.trim.2006.09.022
更新日期：2006-12-01 00:00:00
abstract：BACKGROUND:Blockade of costimulation signaling required for immune response, such as CD40/CD40L and CD28/B7, is a reasonable strategy to prevent rejection and in defined combinations may allow donor specific tolerance. Indeed, in rodents, costimulation blockade with CD28/B7 antagonists or with CD40Ig was able to induce...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2012.10.004
更新日期：2012-12-01 00:00:00
abstract：:Complex polygenic and multifactorial diseases remain a challenge for human geneticists. Here we aim to remind basic definitions of multifactorial diseases and the genetic related concepts underlying classical methods. Knowledge on pathophysiological process and the genetic information available conditions the design o...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/j.trim.2005.03.015
更新日期：2005-08-01 00:00:00
abstract：BACKGROUND:Endothelial microparticles (EMPs) are membrane vesicles shed from endothelial cell in response to injury, activation or apoptosis. Kidney transplantation (KTx) is the treatment of choice for patients with end stage kidney disease (ESKD). The aim of this study was to analyze changes in EMP and serum creatinin...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2014.06.006
更新日期：2014-08-01 00:00:00
abstract：:Persistent rejection in the face of treatment and multiple episodes of rejection are associated with the development of chronic rejection and graft loss in solid organ transplantation. The factors that create an environment for rejection that persists in the face of treatment are as yet not understood. The objective o...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2004.11.001
更新日期：2005-03-01 00:00:00
abstract：:B(io) B(reedding)/O(ttawa) K(alsburg) rats spontaneously develop insulin-dependent type 1 diabetes. Days before BB/OK rats become diabetic, their body seems to be flabby which may be attributed to loss of subcutaneous fat. However, the rats are normoglycemic and manifest 3-4 days later. This observation prompted us to...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2011.01.003
更新日期：2011-05-01 00:00:00
abstract：:Gene polymorphisms of cytotoxic T lymphocyte associated antigen 4 (CTLA4) play an influential role in the graft rejection and long-term clinical outcome of organ transplantation. We investigated the associations of five CTLA4 single nucleotide polymorphisms (SNPs) (rs733618T/C, rs4553808A/G, rs5742909C/T, rs231775G/A,...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2012.02.005
更新日期：2012-06-01 00:00:00
abstract：:Mouse hearts transplanted into MHC disparate donors are usually rejected 1 week after placement. It is widely accepted that alloantigen-reactive helper T lymphocytes (HTL) and cytotoxic T lymphocytes (CTL) are the key mediators of acute allograft rejection. This report demonstrates that the presence or absence of 'tra...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/0966-3274(95)80028-x
更新日期：1995-09-01 00:00:00
