Abstract：
:Previously, we established a murine model, that involves the engraftment of fully allogeneic T cell depleted donor bone marrow cells in sublethally irradiated and single dose anti-CD3 treated recipient mice. These mice developed permanent stable multilineage mixed chimerism and donor-specific tolerance without graft-versus-host disease. Recently, we have shown that donor-specific tolerance is not induced and/or maintained by clonal anergy, neither by a Th1/Th2 shift, nor by suppressor or other regulatory processes. In the present study, we investigated whether clonal deletion plays a role in tolerance induction in our model. We studied the kinetics of TCRVbeta8(+) T cells in BALB/c (H-2L(d+))-->dm2 (H-2L(d-)) chimeras, in which combination of mouse strains TCRVbeta8 predominates the anti-donor response. We found that TCRVbeta8(+) T cells were specifically deleted. To our surprise, this deletion was also found in mixed chimeras, thymectomized prior to the conditioning regimen. We conclude that clonal deletion plays a role in the establishment and maintenance of donor-specific tolerance, and that the thymus is not required for this process. In addition, confocal laser-scanning microscopy clearly showed the presence of abundant amounts of donor T cells and some donor antigen presenting cells in the small intestine in thymectomized chimeras and not in other organs, suggesting that T cell selection might take place in this organ in the absence of the thymus.
journal_name
Transpl Immunoljournal_title
Transplant immunologyauthors
van Pel M,van Breugel DW,van Wijk M,Luypen S,Vingerhoed J,Roholl PJ,Boog CJdoi
10.1016/S0966-3274(02)00157-0subject
Has Abstractpub_date
2003-07-01 00:00:00pages
375-84issue
3-4eissn
0966-3274issn
1878-5492pii
S0966-3274(02)00157-0journal_volume
11pub_type
杂志文章abstract：:HMG-CoA reductase inhibitors, or statins, are effective lipid lowering agents, extensively used in medical practice. Statins have never been shown to be involved in the immune response, although few clinical reports have suggested a better outcome of cardiac transplantation in patients under pravastatin therapy. Major...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/s0966-3274(02)00030-8
更新日期：2002-05-01 00:00:00
abstract：:Despite improvements in the prevention and treatment of graft-versus-host disease (GvHD) this allogeneic immune response is still one of major complications following allogeneic stem cell transplantation (SCT). Identification of patients at risk for the development of acute and chronic GvHD would facilitate early inte...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2012.07.003
更新日期：2012-12-01 00:00:00
abstract：:New immunosuppressive strategies have improved short- and long-term graft survival. The current aim is to decrease the intensity of the immunosuppressive regimen, in an attempt to limit side effects and the direct toxicity of calcineurin inhibitor (CNI) for kidney function. We describe here current experience in liver...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/j.trim.2006.11.007
更新日期：2007-02-01 00:00:00
abstract：:Graft rejection and graft-versus-host disease are leading causes of transplant related mortality despite advancements in immunosuppressive therapy. Mesenchymal stem cells (MSCs) offer a promising addition to immunosuppressive drugs (ISD), while NK-cells are increasingly used as effector cells in graft-versus-leukemia....
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2016.04.008
更新日期：2016-07-01 00:00:00
abstract：:In recent years, the utility of vascular complement factor 4d (C4d) deposition as diagnostic tool for antibody mediated rejection (AMR) after lung transplantation, has become a controversial issue. We aimed to pinpoint the problematic nature of C4d as biomarker with a simple experiment. We quantified C4d in broncho-al...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2020.101352
更新日期：2021-02-01 00:00:00
abstract：:TCR-beta (T cell receptor-beta chain) transgenic mice have altered lymphocyte development. TCR-beta transgenic mice are hyporesponsive to alloantigens in vivo and are deficient in gamma delta T cells. In order to begin a study of the relationship between a deficiency of alloreactive gamma delta cells and the defective...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/0966-3274(94)90063-9
更新日期：1994-09-01 00:00:00
abstract：OBJECTIVE:Acute rejection is a major cause of morbidity and mortality after solid organ transplantation. Therefore, optimizing treatment strategies and improving curative effect is urgent and necessary. Reliable biomarkers for acute rejection and the underlying molecular mechanisms remain to be determined. METHODS:In ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2020.101320
更新日期：2020-10-01 00:00:00
abstract：BACKGROUND:Response to Hepatitis B virus (HBV) vaccination can be diminished in some (50-80%) but not all dialysis patients. We hypothesized, that the response to vaccination on dialysis may correlate with the development of anti-HLA antibodies after renal transplantation and might therefore be a valuable parameter to ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2017.04.003
更新日期：2017-06-01 00:00:00
abstract：BACKGROUND:Peripheral blood CD4+ T cell adenosine triphosphate (ATP) release has been reported to be an adjunct tool to evaluate global cellular immune response in solid-organ transplant recipients. However, the correlation between the ATP level and rejection was controversial. The aim of this prospective clinical stud...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2013.10.008
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:We tested the hypothesis that the best time for genetic modification is while the cell viability of the graft is reduced for long-term preservation. The hemagglutinating virus of Japan (HVJ)-liposome method, a nonviral gene transfer technique, was used with a luciferase gene to test the efficacy of protein i...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/S0966-3274(03)00002-9
更新日期：2003-01-01 00:00:00
abstract：:Over the last 25 years the importance of the humoral presensitization status of transplant recipients has increasingly been recognized. With greater understanding of the precise specificity of the pretransplant antibody status of recipients it is clear that the graft survival figures will improve. The development of n...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/0966-3274(94)90045-0
更新日期：1994-06-01 00:00:00
abstract：OBJECTIVES:To assess the timelines of serum free light chain (sFLC) concentrations and the kappa/lambda light chain (K/L) ratio in heart transplant (HTX) recipients. To analyze the performance of serum protein electrophoresis (SPE), serum immunofixation (sIFE) and sFLC measurements for gammopathy detection following a ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2017.01.003
更新日期：2017-03-01 00:00:00
abstract：:Adoptive cell therapies involving cell manipulation to achieve tolerance are increasingly being studied in animal models and in human trials. We have demonstrated that the specific removal of allo-stimulated dividing cells (or "pruning") promotes long-term allograft survival across a major MHC mismatch in transplant m...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2014.03.002
更新日期：2014-05-01 00:00:00
abstract：AIM:To investigate the influence of immunosuppressants on the fertility of males who undergo renal transplantation as well as on the immune function of their offspring. METHODS:A survey was performed on the fertility of 164 male renal transplant recipients who underwent a long-term treatment with cyclosporine A (CsA),...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2009.10.001
更新日期：2009-12-01 00:00:00
abstract：:RNA interference (RNAi) has recently emerged as an efficient method to silence gene expression in mammalian cells by transfection of small interfering RNAs (siRNAs). The Janus kinase 3 (JAK3) is also pertinent to the development of a new immuno-suppressant. This study aimed to inhibit JAK3 expression using RNAi to det...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2009.02.004
更新日期：2009-05-01 00:00:00
abstract：:Pancreas grafts do not survive for lengthy periods, especially in a high-responder rat combination. Recent data indicated that a combined spleen/pancreas graft protects against acute graft rejection and induces donor-specific tolerance. In this study, we performed a combination spleen/pancreas transplantation using hi...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(01)00049-1
更新日期：2001-10-01 00:00:00
abstract：:The status of "partial" tolerance to organ allografts versus the status of complete tolerance is the main topic of this paper. Progress made in immunosuppression, particularly by use of various lymphocyte depleting agents for "induction therapy", seems to favor the subsequent development of T cells with suppressor/reg...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/j.trim.2004.05.002
更新日期：2004-09-01 00:00:00
abstract：:TIRC7 delivers essential signals during immune activation as antibodies targeting TIRC7 inhibit lymphocyte proliferation and Th1 cytokine expression in vitro and prolonged kidney and heart allograft survival in vivo. Immunohistochemical analysis of biopsy specimens from human renal allografts undergoing rejection desp...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2006.09.027
更新日期：2006-11-01 00:00:00
abstract：:The outcome of hyperacute grade 3-4 steroid-refractory graft-versus-host-disease (SR-GVHD) remains dismal despite a plethora of agents being tried alone or in combination. Following T replete haploidentical transplantation with post-transplantation cyclophosphamide on 75 patients, 10 patients (13%) aged 2-20years, dev...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2016.08.009
更新日期：2016-11-01 00:00:00
abstract：BACKGROUND:Blockade of costimulation signaling required for immune response, such as CD40/CD40L and CD28/B7, is a reasonable strategy to prevent rejection and in defined combinations may allow donor specific tolerance. Indeed, in rodents, costimulation blockade with CD28/B7 antagonists or with CD40Ig was able to induce...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2012.10.004
更新日期：2012-12-01 00:00:00
abstract：:Mouse hearts transplanted into MHC disparate donors are usually rejected 1 week after placement. It is widely accepted that alloantigen-reactive helper T lymphocytes (HTL) and cytotoxic T lymphocytes (CTL) are the key mediators of acute allograft rejection. This report demonstrates that the presence or absence of 'tra...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/0966-3274(95)80028-x
更新日期：1995-09-01 00:00:00
abstract：:The ImmuKnow assay measures cell-mediated immunity, quantifying ATP production from peripheral blood CD4+T-cells in solid-organ transplant patients who undergo immunosuppressive therapy. We aimed to measure functional immunity in lung transplant recipients and correlate Immuknow values with immunosuppression levels, p...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2016.04.001
更新日期：2016-07-01 00:00:00
abstract：:Previous work on the facilitation of xenogeneic and allogeneic bone marrow engraftment in irradiated mice and dogs with transferrins allowed the development of a model for induction of an apparently durable state of immunological unresponsiveness or 'tolerance' in chemically immunosuppressed mice. The system is based ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(96)80051-7
更新日期：1996-12-01 00:00:00
abstract：:We investigated the dynamics of the CD4+ and CD8+ lymphocyte subsets, and the expression of activation markers in cardiac transplant recipients. We tested 132 peripheral blood samples from 62 cardiac transplant recipients using fluorescent staining and flow cytometry analysis. The results were correlated with histolog...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(02)00072-2
更新日期：2002-11-01 00:00:00
abstract：:Desferrioxamine (DFO), commonly used in therapy as a chelator of ferric ion in disorders of iron overload, is a potent inhibitor of human cytomegalovirus (HCMV) replication in cultured fibroblast cells. Moreover, DFO has immunomodulatory activity both in vitro and in vivo. We studied DFO effects on HCMV replication in...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/0966-3274(95)80017-4
更新日期：1995-12-01 00:00:00
abstract：:The mechanisms by which alloreactive memory T-cells are generated in non-sensitized individuals have begun to be elucidated. It is generally accepted that a very high level of crossreactivity is an essential feature of the T-cell receptor. Indeed it has recently been shown that alloreactivity from viral specific memor...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/j.trim.2010.06.008
更新日期：2010-08-01 00:00:00
abstract：:Previously, we developed a well-tolerated single-day protocol for induction of stable multilineage chimerism and permanent donor-specific tolerance across major histocompatibility complex (MHC) barriers, with preservation of the host's normal immune responses. In our murine model, recipient mice were treated with a si...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(98)80039-7
更新日期：1998-09-01 00:00:00
abstract：:Desensitization (DS) is widely used to decrease PRA in solid organs transplant candidates (TC). Various numbers of cycles of DS are required to reduce or eliminate donor specific antibodies (DSA). The goal of this study was to investigate if there was a correlation between polymorphism (PM) of some cytokine genes and ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2009.03.002
更新日期：2009-07-01 00:00:00
abstract：:FTY720 alters lymphocyte recirculation and homing by interfering with S1P receptors on lymphocytes, possibly in combination with chemokine receptors, and induces a decrease in PBL counts. In fresh, whole blood samples of 14 kidney transplant patients, we analyzed by flow cytometry the effect of FTY on the number of NK...
journal_title：Transplant immunology
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.trim.2006.02.002
更新日期：2006-04-01 00:00:00
abstract：BACKGROUND:Xenotransplantation is an appealing alternative to human allotransplantation because of a worldwide shortage of organs. One of the obstacles for xenografts is cellular rejection by the innate immune system, comprised of NK cells, monocytes, and macrophages. In this study the inhibitory function of HLA-G1, a ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2014.12.004
更新日期：2015-03-01 00:00:00
