当前位置： SCI文献检索 > TRANSPLANT IMMUNOLOGY期刊下所有文献 > Downregulation of Janus kinase 3 expression by small interfering RNA in rat composite tissue allotransplantation.

Downregulation of Janus kinase 3 expression by small interfering RNA in rat composite tissue allotransplantation.

Abstract：

:RNA interference (RNAi) has recently emerged as an efficient method to silence gene expression in mammalian cells by transfection of small interfering RNAs (siRNAs). The Janus kinase 3 (JAK3) is also pertinent to the development of a new immuno-suppressant. This study aimed to inhibit JAK3 expression using RNAi to determine allograft tolerance. To silence JAK3 expression, one dsRNA was tested to incorporate the JAK3 mRNA sequence. The expression vector containing the pre-mRNA expression cassette was transfected into rat basophilic leukemia cell line, RBL-2H3, for RNAi analysis (in vitro). The alloskin and composite tissue allograft were then transplanted to recipients using RNAi protocol to determine the allograft tolerance (in vivo). The results showed effective in vitro and in vivo downregulation of JAK3 expression by RNAi. Moreover, the histology of alloskin graft and composite tissue allograft (in vivo) under the siRNA showed more prominently diminished inflammatory infiltration than the control group. This is the first time in the literature that the suppressive effect of JAK3 silenced by siRNA has been tested both in vitro and in vivo, and shows that siRNA is capable of specific and functional silencing in allograft rejection.

journal_name

Transpl Immunol

journal_title

Transplant immunology

authors

Chang KP,Lai CH,Huang SH,Tai HT,Chang LL,Lin SD,Hwang SL,Lai CS

doi

10.1016/j.trim.2009.02.004

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

27-32

issue

1

eissn

0966-3274

issn

1878-5492

pii

S0966-3274(09)00017-3

journal_volume

21

pub_type

杂志文章

相关文献

TRANSPLANT IMMUNOLOGY文献大全
  • Association between transforming growth factor beta-1 +869T/C polymorphism and acute rejection of solid organ allograft: A meta-analysis and systematic review.

    abstract：BACKGROUND:Transforming growth factor beta-1(TGFB1) is involved in the acute rejection (AR) episodes of solid organ transplant recipients. However, results from published studies on the association between donor/recipient TGFB1 +869T/C polymorphism and AR risk are conflicting and inconclusive. METHODS:PUBMED, EMBASE, ...

    journal_title：Transplant immunology

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.trim.2014.01.001

    authors： Ge YZ,Yu P,Jia RP,Wu R,Ding AX,Li LP,Zhao Y,Feng YM,Gui ZL,Liao S

    更新日期：2014-03-01 00:00:00

  • Effects of recombinant thrombomodulin therapy and soluble human leukocyte antigen-G levels during hematopoietic stem cell transplantation.

    abstract：BACKGROUND:Conditioning chemotherapies for hematopoietic stem cell transplantation (HSCT), especially those that include total body irradiation, can result in serious complications such as graft-versus-host disease (GVHD). Human leukocyte antigen G (HLA-G) is a non-classical class I molecule with multiple immunoregulat...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2018.12.001

    authors： Nomura S,Ito T,Katayama Y,Ota S,Hayashi K,Fujita S,Satake A,Ishii K

    更新日期：2019-04-01 00:00:00

  • Intragraft mRNA cytotoxic molecule expression in renal allograft recipients.

    abstract：:The gene expression of the cytotoxic T-cell molecules perforin, granzyme B and Fas ligand are associated with acute rejection in renal allograft recipients. Several immune mechanisms are linked to severe systemic inflammation in brain-dead organ donors. We examined the mRNA expression of these T-cell activation biomar...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2008.12.002

    authors： Carstens J,Ozbay A,Tørring C,Hansen HE

    更新日期：2009-03-01 00:00:00

  • Efficient peripheral construction of functional human regulatory CD4(+)CD25(high)Foxp3(+) T cells in NOD/SCID mice grafted with fetal human thymus/liver tissues and CD34(+) cells.

    abstract：:Regulatory T cells, especially CD4(+)CD25(+) regulatory T cells are critical regulators of immune tolerance in humans and mice. Mice with humanized immunity have been developed by various transplantation strategies of human tissues or cells related to immunity, which are being extensively applied in biomedical researc...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2011.08.003

    authors： Duan K,Zhang B,Zhang W,Zhao Y,Qu Y,Sun C,Zhao Y

    更新日期：2011-12-01 00:00:00

  • Inflammatory immune responses in a reproducible mouse brain death model.

    abstract：BACKGROUND:Brain death impairs donor organ quality and accelerates immune responses after transplantation. Detailed aspects of immune activation following brain death remain unclear. We have established a mouse model and investigated the immediate consequences of brain death and anesthesia on immune responses. METHODS...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2012.04.002

    authors： Floerchinger B,Yuan X,Jurisch A,Timsit MO,Ge X,Lee YL,Schmid C,Tullius SG

    更新日期：2012-08-01 00:00:00

  • Tim-3 expression in human kidney allografts.

    abstract：BACKGROUND:Tim-3 was recently described as a Th1-specific molecule, participating in the regulation of immune responses and in the induction of allograft tolerance. Here, we studied Tim-3 mRNA expression together with molecular markers of T-cell activation and cytotoxicity, in rejected human kidney grafts. METHODS:Twe...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2006.11.003

    authors： Ponciano VC,Renesto PG,Nogueira E,Rangel EB,Cenedeze MA,Franco MF,Câmara NO,Pacheco-Silva A

    更新日期：2007-04-01 00:00:00

  • Unresponsiveness to human leukocytes in immunosuppressed mice by combined donor-derived human transferrin and antigens.

    abstract：:Previous work on the facilitation of xenogeneic and allogeneic bone marrow engraftment in irradiated mice and dogs with transferrins allowed the development of a model for induction of an apparently durable state of immunological unresponsiveness or 'tolerance' in chemically immunosuppressed mice. The system is based ...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/s0966-3274(96)80051-7

    authors： Pierpaoli W,Lesnikov VA,Lesnikova MP,Arrighi S,Bardotti A

    更新日期：1996-12-01 00:00:00

  • Natural anti-galactose alpha1,3 galactose antibodies delay, but do not prevent the acceptance of extracellular matrix xenografts.

    abstract：:Naturally occurring antibodies to the galactose alpha1,3 galactose (alpha gal) epitope expressed on xenogeneic grafts are a major barrier to organ transplantation in humans. Porcine small intestinal submucosa (SIS) expresses the alpha gal epitope and is currently being used as a bioscaffold for tissue remodeling. To e...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/s0966-3274(01)00044-2

    authors： Raeder RH,Badylak SF,Sheehan C,Kallakury B,Metzger DW

    更新日期：2002-06-01 00:00:00

  • Statins as immunomodulators.

    abstract：:HMG-CoA reductase inhibitors, or statins, are effective lipid lowering agents, extensively used in medical practice. Statins have never been shown to be involved in the immune response, although few clinical reports have suggested a better outcome of cardiac transplantation in patients under pravastatin therapy. Major...

    journal_title：Transplant immunology

    pub_type： 杂志文章,评审

    doi：10.1016/s0966-3274(02)00030-8

    authors： Mach F

    更新日期：2002-05-01 00:00:00

  • HMGB1 blockade significantly improves luminal fibrous obliteration in a murine model of bronchiolitis obliterans syndrome.

    abstract：BACKGROUND:Although high-mobility group box-1 (HMGB1), which is a nuclear protein, was reported to enhance the allogeneic responses in transplantation, the effect of HMGB1 on bronchiolitis obliterans syndrome (BOS) is unknown. METHODS:A murine heterotopic tracheal transplantation model was used. Protein concentrations...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2018.11.007

    authors： Takamori S,Shoji F,Okamoto T,Kozuma Y,Matsubara T,Haratake N,Akamine T,Katsura M,Takada K,Toyokawa G,Tagawa T,Maehara Y

    更新日期：2019-04-01 00:00:00

  • Elevated intragraft expression of innate immunity and cell death-related markers is a risk factor for adverse graft outcome.

    abstract：BACKGROUND:Molecules of the innate immune response are increasingly recognized as important mediators in allograft injury during and after kidney transplantation. We therefore aimed to establish the relationship between the expression of these genes at implantation, during an acute rejection (AR) and on graft outcome. ...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2018.02.009

    authors： Yang J,Snijders MLH,Haasnoot GW,van Kooten C,Mallat M,de Fijter JW,Clahsen-van Groningen MC,Claas FHJ,Eikmans M

    更新日期：2018-06-01 00:00:00

  • Manipulation of skin graft rejection in alloimmune mice by anti-VCAM-1:VLA-4 but not anti-ICAM-1:LFA-1 monoclonal antibodies.

    abstract：:C3H mice were immunized by repeated skin grafting with B10.BR tail skin. Ten days after the last immunization mice received 100 micrograms (intravenously) of a variety of different monoclonal antibodies (mAbs: anti-ICAM-1, -LFA-1, -VCAM-1, -VLA-4), alone or in combination, followed by further B10.BR skin grafts. Contr...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/0966-3274(95)80007-7

    authors： Gorcyznski RM,Chung S,Fu XM,Levy G,Sullivan B,Chen Z

    更新日期：1995-03-01 00:00:00

  • Current cellular immunological hurdles in pig-to-primate xenotransplantation.

    abstract：:In this review, we summarize the work published over the last few years relative to cellular immunological hurdles encountered specifically in pig-to-primate xenotransplantation models. The works summarized here cover both the innate and adaptative cellular immune response as well as strategies to overcome them and co...

    journal_title：Transplant immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.trim.2008.10.006

    authors： Le Bas-Bernardet S,Blancho G

    更新日期：2009-06-01 00:00:00

  • The concept of "partial" clinical tolerance.

    abstract：:The status of "partial" tolerance to organ allografts versus the status of complete tolerance is the main topic of this paper. Progress made in immunosuppression, particularly by use of various lymphocyte depleting agents for "induction therapy", seems to favor the subsequent development of T cells with suppressor/reg...

    journal_title：Transplant immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.trim.2004.05.002

    authors： Cortesini R,Suciu-Foca N

    更新日期：2004-09-01 00:00:00

  • Effect of desensitization in solid organ transplant recipients depends on some cytokines genes polymorphism.

    abstract：:Desensitization (DS) is widely used to decrease PRA in solid organs transplant candidates (TC). Various numbers of cycles of DS are required to reduce or eliminate donor specific antibodies (DSA). The goal of this study was to investigate if there was a correlation between polymorphism (PM) of some cytokine genes and ...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2009.03.002

    authors： Lobashevsky AL,Manwaring JE,Travis MM,Nord BL,Higgins NG,Serov YA,Arnoff TS,Hommel-Berrey GA,Goggins WC,Taber TE,Carter CB Sr,Smith DS,Wozniak TC,O'Donnell JA,Turrentine MW

    更新日期：2009-07-01 00:00:00

  • Elevated mRNA levels of CTLA-4, FoxP3, and granzyme B in BAL, but not in blood, during acute rejection of lung allografts.

    abstract：INTRODUCTION:Regulatory T cells (Tregs) have been related to acute rejection as have the cytotoxic T cells, their immunological counterpart. High expression of cytotoxic markers has been related to acute rejection incidents following both kidney and intestine transplantation, while the correlation between FoxP3 express...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2010.06.011

    authors： Madsen CB,Nørgaard A,Iversen M,Ryder LP

    更新日期：2010-10-01 00:00:00

  • Quantitative detection of changes in cytokine gene expression in peripheral blood mononuclear cells correlates with and precedes acute rejection in renal transplant recipients.

    abstract：:Immunological monitoring of transplant recipients is an attractive concept. Cytokines provide an obvious focus for research, as they are central to the human immune response. This study aimed to identify cytokines whose sequential gene expression differentiated rejectors from non-rejectors immediately following renal ...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2005.02.003

    authors： Gibbs PJ,Tan LC,Sadek SA,Howell WM

    更新日期：2005-06-01 00:00:00

  • Dissociation of mouse cardiac transplant rejection and donor alloantigen-specific T cell responsiveness.

    abstract：:Mouse hearts transplanted into MHC disparate donors are usually rejected 1 week after placement. It is widely accepted that alloantigen-reactive helper T lymphocytes (HTL) and cytotoxic T lymphocytes (CTL) are the key mediators of acute allograft rejection. This report demonstrates that the presence or absence of 'tra...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/0966-3274(95)80028-x

    authors： Bishop DK,DeBruyne LA,Chan S,Xu S,Eichwald EJ

    更新日期：1995-09-01 00:00:00

  • An alternative conditioning regimen for induction of specific skin graft tolerance across full major histocompatibility complex barriers.

    abstract：:Previously, we developed a well-tolerated single-day protocol for induction of stable multilineage chimerism and permanent donor-specific tolerance across major histocompatibility complex (MHC) barriers, with preservation of the host's normal immune responses. In our murine model, recipient mice were treated with a si...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/s0966-3274(98)80039-7

    authors： de Vries-van der Zwan A,van der Pol MA,de Waal LP,Boog CJ

    更新日期：1998-09-01 00:00:00

  • mir-155 expression is downregulated in kidney transplant patients with human cytomegalovirus infection.

    abstract：:MicroRNAs are small, noncoding RNAs that control expression of target genes through inhibiting protein translation or inducing degradation of mRNA transcripts of target genes. According to the latest update, 2578 unique mature miRNAs are currently annotated in the human genome and participate in the regulation of mult...

    journal_title：Transplant immunology

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.trim.2017.06.002

    authors： Bergallo M,Gambarino S,Montanari P,Daprà V,Rassu M,Galliano I,Ravanini P

    更新日期：2017-08-01 00:00:00

  • Donor-specific tolerance in a murine model: the result of extra-thymic T cell deletion?

    abstract：:Previously, we established a murine model, that involves the engraftment of fully allogeneic T cell depleted donor bone marrow cells in sublethally irradiated and single dose anti-CD3 treated recipient mice. These mice developed permanent stable multilineage mixed chimerism and donor-specific tolerance without graft-v...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/S0966-3274(02)00157-0

    authors： van Pel M,van Breugel DW,van Wijk M,Luypen S,Vingerhoed J,Roholl PJ,Boog CJ

    更新日期：2003-07-01 00:00:00

  • Microvascular integrity plays an important role for graft survival after experimental skin transplantation.

    abstract：BACKGROUND:Every transplanted organ relies on a reliable and sound vascular system. Therefore, our study focused on the investigation if platelet inhibition alone or combined with mTOR-inhibition has a beneficial effect on the microvascular integrity in allogeneic murine skin grafts. METHODS:Skin transplantation was p...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2015.09.005

    authors： Motsch B,Heim C,Koch N,Ramsperger-Gleixner M,Weyand M,Ensminger SM

    更新日期：2015-11-01 00:00:00

  • Fas Ligand gene transfer enhances the survival of tissue-engineered chondrocyte allografts in mini-pigs.

    abstract：BACKGROUND AND PURPOSE:Since the Fas/Fas Ligand (FasL) interaction has been recognized as an apoptotic pathway, it eliminates the activated T cells and promotes the survival of grafts. In this study, the effect of FasL transfection of pig chondrocytes on allogeneic transplantation was examined in vitro and in vivo. ME...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2008.02.001

    authors： Xie GH,Wang SJ,Wang Y,Zhang Y,Zhang HZ,Jin S,Wang QF,Liu ZC,Ge HL

    更新日期：2008-05-01 00:00:00

  • FTY720 treatment of kidney transplant patients: a differential effect on B cells, naïve T cells, memory T cells and NK cells.

    abstract：:FTY720 alters lymphocyte recirculation and homing by interfering with S1P receptors on lymphocytes, possibly in combination with chemokine receptors, and induces a decrease in PBL counts. In fresh, whole blood samples of 14 kidney transplant patients, we analyzed by flow cytometry the effect of FTY on the number of NK...

    journal_title：Transplant immunology

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1016/j.trim.2006.02.002

    authors： Vaessen LM,van Besouw NM,Mol WM,Ijzermans JN,Weimar W

    更新日期：2006-04-01 00:00:00

  • Prolonged ischemia elicits acute allograft rejection involved in CXCR3 activation in rat kidney transplants.

    abstract：BACKGROUND:Acute rejection is a major obstacle in patients with prolonged ischemia in deceased-donor renal transplantation. Chemokines and their receptors play a critical role in leukocyte trafficking, resulting in allograft rejection; therefore, the role of chemokine receptor CXCR3 in acute rejection induced by prolon...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2015.08.001

    authors： Zou XF,Song B,Duan JH,Hu ZD,Cui ZL,Gu C

    更新日期：2015-10-01 00:00:00

  • Immediate impact of COVID-19 on transplant activity in the Netherlands.

    abstract：:The rapid emergence of the COVID-19 pandemic is unprecedented and poses an unparalleled obstacle in the sixty-five year history of organ transplantation. Worldwide, the delivery of transplant care is severely challenged by matters concerning - but not limited to - organ procurement, risk of SARS-CoV-2 transmission, sc...

    journal_title：Transplant immunology

    pub_type： 社论

    doi：10.1016/j.trim.2020.101304

    authors： de Vries APJ,Alwayn IPJ,Hoek RAS,van den Berg AP,Ultee FCW,Vogelaar SM,Haase-Kromwijk BJJM,Heemskerk MBA,Hemke AC,Nijboer WN,Schaefer BS,Kuiper MA,de Jonge J,van der Kaaij NP,Reinders MEJ

    更新日期：2020-08-01 00:00:00

  • T and B cell crossmatching using three-colour flow cytometry.

    abstract：:We have developed a highly sensitive method for the simultaneous crossmatching of T and B cells using three-colour flow cytometry. This method identifies T and B cell populations in a lymphocyte sample and analyses each for the presence of bound patient IgG alloantibodies. A fluorescein isothiocyanate conjugated rabbi...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/s0966-3274(96)80018-9

    authors： Robson A,Martin S

    更新日期：1996-09-01 00:00:00

  • Evaluation of histological damage of solid organs after donor preconditioning with thymoglobulin in an experimental rat model.

    abstract：:Rabbit anti-rat thymoglobulin (rATG) administered to donors with brain death (BD) may improve organs quality. We explored the effects of rATG administered to BD donors in the histology of heart, lungs and small bowel in a rat experimental model. Animals were randomly assigned to 3 groups: V (n=5) no BD, 2h ventilation...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2013.04.002

    authors： Cicora F,Stringa P,Guerrieri D,Vásquez D,Toniolo F,Roberti J,Raimondi C

    更新日期：2013-06-01 00:00:00

  • The impact of pharmacogenomic factors on acute persistent rejection in adult lung transplant patients.

    abstract：:Persistent rejection in the face of treatment and multiple episodes of rejection are associated with the development of chronic rejection and graft loss in solid organ transplantation. The factors that create an environment for rejection that persists in the face of treatment are as yet not understood. The objective o...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2004.11.001

    authors： Zheng HX,Zeevi A,McCurry K,Schuetz E,Webber S,Ristich J,Zhang J,Iacono A,Dauber J,McDade K,Zaldonis D,Lamba J,Burckart GJ

    更新日期：2005-03-01 00:00:00

  • Suppression of human macrophage-mediated cytotoxicity by transgenic swine endothelial cell expression of HLA-G.

    abstract：BACKGROUND:Xenotransplantation is an appealing alternative to human allotransplantation because of a worldwide shortage of organs. One of the obstacles for xenografts is cellular rejection by the innate immune system, comprised of NK cells, monocytes, and macrophages. In this study the inhibitory function of HLA-G1, a ...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2014.12.004

    authors： Esquivel EL,Maeda A,Eguchi H,Asada M,Sugiyama M,Manabe C,Sakai R,Matsuura R,Nakahata K,Okuyama H,Miyagawa S

    更新日期：2015-03-01 00:00:00