当前位置： SCI文献检索 > TRANSPLANT IMMUNOLOGY期刊下所有文献 > Elevated mRNA levels of CTLA-4, FoxP3, and granzyme B in BAL, but not in blood, during acute rejection of lung allografts.

Elevated mRNA levels of CTLA-4, FoxP3, and granzyme B in BAL, but not in blood, during acute rejection of lung allografts.

Abstract：

INTRODUCTION:Regulatory T cells (Tregs) have been related to acute rejection as have the cytotoxic T cells, their immunological counterpart. High expression of cytotoxic markers has been related to acute rejection incidents following both kidney and intestine transplantation, while the correlation between FoxP3 expression and acute rejection is still being debated. Some studies have been performed on blood samples from lung-transplanted patients, while others have investigated the local immune response in the lungs by analysing broncho-alveolar-lavage (BAL) fluids or biopsies. Biopsies are considered the gold standard in diagnosis of acute rejection. AIM:The aim was to measure the expression of both Treg (FoxP3, CD25 and CTLA-4) and cytotoxic (granzyme B, granulysin, and perforin) markers in BAL and blood samples from lung-transplanted patients to investigate the possible relation of expression and acute rejection incidents in order to develop a non-invasive diagnostic method for acute rejection. MATERIALS AND METHODS:24 lung-transplanted patients were included in this 6-month cross-section study. BAL and blood samples were analysed for FoxP3, CD25, CTLA-4, granzyme B, granulysin, perforin, CD4 and CD8 mRNA by QRT-PCR with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as primary reference. RESULTS:We demonstrate that the mRNA levels in BAL relative to GAPDH of nearly all markers are elevated during acute rejection; CTLA-4, FoxP3, and granzyme B significantly, while a strong tendency is seen among the others. No significant differences were detected in blood. CONCLUSION:CTLA-4, FoxP3 and Granzyme B mRNA levels are elevated during acute rejection in BAL, but not in blood, following lung transplantation, indicating that evaluation of Treg and cytotoxic marker expression in BAL can be used in the assessment of allograft rejection state.

journal_name

Transpl Immunol

journal_title

Transplant immunology

authors

Madsen CB,Nørgaard A,Iversen M,Ryder LP

doi

10.1016/j.trim.2010.06.011

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

26-32

issue

1

eissn

0966-3274

issn

1878-5492

pii

S0966-3274(10)00083-3

journal_volume

24

pub_type

杂志文章

相关文献

TRANSPLANT IMMUNOLOGY文献大全
  • TIRC7 is induced in rejected human kidneys and anti-TIRC7 mAb with FK506 prolongs survival of kidney allografts in rats.

    abstract：:TIRC7 delivers essential signals during immune activation as antibodies targeting TIRC7 inhibit lymphocyte proliferation and Th1 cytokine expression in vitro and prolonged kidney and heart allograft survival in vivo. Immunohistochemical analysis of biopsy specimens from human renal allografts undergoing rejection desp...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2006.09.027

    authors： Kumamoto Y,Tamura A,Volk HD,Reinke P,Löhler J,Tullius SG,Utku N

    更新日期：2006-11-01 00:00:00

  • Long-lasting donor passenger leukocytes after hepatic and intestinal transplantation in rats.

    abstract：BACKGROUND:Donor passenger leukocytes (DPLs) that migrate after organ transplantation stimulate the recipient immune system and normally cause rejection and graft vs. host reaction. However, DPLs also contribute to the unresponsiveness to the donor organ. The quantity and quality of these migrating cells are considered...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2003.10.001

    authors： Inoue S,Tahara K,Kaneko T,Ajiki T,Takeda S,Sato Y,Hakamata Y,Murakami T,Takahashi M,Kaneko M,Kobayashi E

    更新日期：2004-01-01 00:00:00

  • Combined donor leucocyte administration and immunosuppressive drug treatment for survival of rat heart allografts.

    abstract：BACKGROUND:Donor leucocytes (DL) play an important role in rat liver transplant tolerance and their postoperative administration can convert rejection to tolerance. They appear to induce early activation, altered patterns of infiltration and death of recipient alloreactive T cells. The ability of immunosuppressive drug...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2004.05.003

    authors： den Dulk M,Wang C,Li J,Clark DA,Hibberd AD,Terpstra OT,McCaughan GW,Bishop GA

    更新日期：2004-11-01 00:00:00

  • ABO incompatible renal transplantation following lung transplantation.

    abstract：:We present management strategies utilised for the first case of an urgent live-donor ABO incompatible B blood group renal transplant, in a patient with a prior A blood group lung transplant for cystic fibrosis. Three years on, renal function is excellent and stable, whilst lung function has improved. ...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2016.09.002

    authors： Snell GI,Davis AK,Menahem S,Kotecha S,Whitford HM,Levvey BJ,Paraskeva M,Webb A,Westall GW,Walker RG

    更新日期：2016-11-01 00:00:00

  • Strategies in analysis of the genetic component of multifactorial diseases; biostatistical aspects.

    abstract：:Complex polygenic and multifactorial diseases remain a challenge for human geneticists. Here we aim to remind basic definitions of multifactorial diseases and the genetic related concepts underlying classical methods. Knowledge on pathophysiological process and the genetic information available conditions the design o...

    journal_title：Transplant immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.trim.2005.03.015

    authors： Barnetche T,Gourraud PA,Cambon-Thomsen A

    更新日期：2005-08-01 00:00:00

  • Immediate impact of COVID-19 on transplant activity in the Netherlands.

    abstract：:The rapid emergence of the COVID-19 pandemic is unprecedented and poses an unparalleled obstacle in the sixty-five year history of organ transplantation. Worldwide, the delivery of transplant care is severely challenged by matters concerning - but not limited to - organ procurement, risk of SARS-CoV-2 transmission, sc...

    journal_title：Transplant immunology

    pub_type： 社论

    doi：10.1016/j.trim.2020.101304

    authors： de Vries APJ,Alwayn IPJ,Hoek RAS,van den Berg AP,Ultee FCW,Vogelaar SM,Haase-Kromwijk BJJM,Heemskerk MBA,Hemke AC,Nijboer WN,Schaefer BS,Kuiper MA,de Jonge J,van der Kaaij NP,Reinders MEJ

    更新日期：2020-08-01 00:00:00

  • Effect of desensitization in solid organ transplant recipients depends on some cytokines genes polymorphism.

    abstract：:Desensitization (DS) is widely used to decrease PRA in solid organs transplant candidates (TC). Various numbers of cycles of DS are required to reduce or eliminate donor specific antibodies (DSA). The goal of this study was to investigate if there was a correlation between polymorphism (PM) of some cytokine genes and ...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2009.03.002

    authors： Lobashevsky AL,Manwaring JE,Travis MM,Nord BL,Higgins NG,Serov YA,Arnoff TS,Hommel-Berrey GA,Goggins WC,Taber TE,Carter CB Sr,Smith DS,Wozniak TC,O'Donnell JA,Turrentine MW

    更新日期：2009-07-01 00:00:00

  • Downregulation of Janus kinase 3 expression by small interfering RNA in rat composite tissue allotransplantation.

    abstract：:RNA interference (RNAi) has recently emerged as an efficient method to silence gene expression in mammalian cells by transfection of small interfering RNAs (siRNAs). The Janus kinase 3 (JAK3) is also pertinent to the development of a new immuno-suppressant. This study aimed to inhibit JAK3 expression using RNAi to det...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2009.02.004

    authors： Chang KP,Lai CH,Huang SH,Tai HT,Chang LL,Lin SD,Hwang SL,Lai CS

    更新日期：2009-05-01 00:00:00

  • The number of regulatory T cells in transbronchial lung allograft biopsies is related to FoxP3 mRNA levels in bronchoalveolar lavage fluid and to the degree of acute cellular rejection.

    abstract：BACKGROUND:The transcription factor Forkhead Box P3 (FoxP3) is a marker of regulatory T cells (Tregs) - a subset of T cells known to suppress a wide range of immune responses. These cells are considered to be pivotal for the induction of tolerance to donor antigens in human allografts. We aimed to correlate the number ...

    journal_title：Transplant immunology

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.trim.2013.08.002

    authors： Krustrup D,Madsen CB,Iversen M,Engelholm L,Ryder LP,Andersen CB

    更新日期：2013-12-01 00:00:00

  • Malononitrilamides and tacrolimus additively prevent acute rejection in rat cardiac allografts.

    abstract：:The novel immunosuppressive agents malononitrilamides (MNA) 279 and 715 are derivatives of A77 1726, the primary metabolite of leflunomide. The effects of these agents have been previously demonstrated in rat skin and cardiac allo- and xenotransplant models. The aim of this study was to evaluate the combination of MNA...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/s0966-3274(99)80036-7

    authors： Qi Z,Simanaitis M,Ekberg H

    更新日期：1999-09-01 00:00:00

  • Immune regulation by regulatory T cells: implications for transplantation.

    abstract：:The induction of antigen-specific T cell tolerance and its maintenance in the periphery are critical for the immune system to prevent autoaggressive immune responses. Our current state of knowledge about the immunoregulatory mechanisms responsible for T cell tolerance in the periphery offers new possibilities for immu...

    journal_title：Transplant immunology

    pub_type： 杂志文章,评审

    doi：10.1016/S0966-3274(03)00057-1

    authors： Jonuleit H,Adema G,Schmitt E

    更新日期：2003-07-01 00:00:00

  • Effects of different serum-levels of ATG after unrelated donor umbilical cord blood transplantation.

    abstract：:We determined total rabbit-IgG (r-ATG) levels in serum samples before (day 0) and after (day 11 and day 25) unrelated donor umbilical cord blood transplantation (UCBT). Most patients (27/41) suffered from a haematological malignancy. There were 25 children and 16 adults. All patients received rabbit anti-thymocyte glo...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2012.06.003

    authors： Remberger M,Persson M,Mattsson J,Gustafsson B,Uhlin M

    更新日期：2012-08-01 00:00:00

  • Comparative analysis of dendritic cells and anti-CD3/CD28 expanded regulatory T cells for application in transplantation.

    abstract：:The containment of direct and indirect recognition of donor alloantigens by enhancement of the number and activity of regulatory T cells (Tregs) has been one of the promising approaches to achieve transplant tolerance. Two major methods, dendritic cell (DC) and anti-CD3/CD28 antibodies (Abs) have been introduced for e...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2009.07.004

    authors： Zeng M,Guinet E,Nouri-Shirazi M

    更新日期：2009-12-01 00:00:00

  • Early onset of rejection in concordant hamster xeno hearts display signs of necrosis, but not apoptosis, correlating to the phosphocreatine concentration.

    abstract：BACKGROUND:The importance of apoptosis contra necrosis for ischemia/reperfusion (RP) and acute rejection in concordant rodent xenotransplantation is largely unknown. We explored this question by comparing rodent allo and concordant xenotransplants with different morphological methods to detect apoptosis and biochemical...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/S0966-3274(03)00018-2

    authors： Lukes DJ,Tivesten A,Wilton J,Lundgren A,Rakotonirainy O,Kjellström C,Isgaard J,Karlsson-Parra A,Soussi B,Olausson M

    更新日期：2003-10-01 00:00:00

  • T cell receptor (beta chain) transgenic mice have selective deficits in gamma delta T cell subpopulations.

    abstract：:TCR-beta (T cell receptor-beta chain) transgenic mice have altered lymphocyte development. TCR-beta transgenic mice are hyporesponsive to alloantigens in vivo and are deficient in gamma delta T cells. In order to begin a study of the relationship between a deficiency of alloreactive gamma delta cells and the defective...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/0966-3274(94)90063-9

    authors： Rimm IJ,Fruman DA,Abhyankar S,Sakamoto H,Orme IM,Milstone D,Seidman JG,Ferrara JL

    更新日期：1994-09-01 00:00:00

  • T cell costimulation blockade for hyperacute steroid refractory graft versus-host disease in children undergoing haploidentical transplantation.

    abstract：:The outcome of hyperacute grade 3-4 steroid-refractory graft-versus-host-disease (SR-GVHD) remains dismal despite a plethora of agents being tried alone or in combination. Following T replete haploidentical transplantation with post-transplantation cyclophosphamide on 75 patients, 10 patients (13%) aged 2-20years, dev...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2016.08.009

    authors： Jaiswal SR,Zaman S,Chakrabarti A,Sehrawat A,Bansal S,Gupta M,Chakrabarti S

    更新日期：2016-11-01 00:00:00

  • Added effects of dexamethasone and mesenchymal stem cells on early Natural Killer cell activation.

    abstract：:Graft rejection and graft-versus-host disease are leading causes of transplant related mortality despite advancements in immunosuppressive therapy. Mesenchymal stem cells (MSCs) offer a promising addition to immunosuppressive drugs (ISD), while NK-cells are increasingly used as effector cells in graft-versus-leukemia....

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2016.04.008

    authors： Michelo CM,Fasse E,van Cranenbroek B,Linda K,van der Meer A,Abdelrazik H,Joosten I

    更新日期：2016-07-01 00:00:00

  • Short-term anti-CD25 monoclonal antibody treatment and neogenetic CD4(+)CD25(high) regulatory T cells in kidney transplantation.

    abstract：:CD4(+)CD25(high) T cells named regulatory T (Treg) cells are generated and play a key role in the induction and maintenance of transplant tolerance in organ recipients. Interleukin-2 (IL-2) enhance the development of effector cells and is essential for generation of Treg cells. The effect of the anti-CD25 monoclonal a...

    journal_title：Transplant immunology

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.trim.2008.01.005

    authors： Wang Z,Xiao L,Shi BY,Qian YY,Bai HW,Chang JY,Cai M

    更新日期：2008-04-01 00:00:00

  • Anti-cell surface endothelial antibodies in sera from cardiac and kidney transplant recipients: association with chronic rejection.

    abstract：:The aetiologies of accelerated or transplant-associated coronary artery disease (TxCAD) following cardiac transplantation and chronic rejection following renal transplantation remain ill-defined. Previous studies have used Western blotting to demonstrate an association between the formation of anti-endothelial (anti-E...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/s0966-3274(97)80021-4

    authors： Ferry BL,Welsh KI,Dunn MJ,Law D,Proctor J,Chapel H,Yacoub MH,Rose ML

    更新日期：1997-03-01 00:00:00

  • Impact of vaccine therapy using nuclear histone H1 on allograft survival in experimental organ transplantation.

    abstract：BACKGROUND:We recently reported that autoreactive antibody (Ab) against nuclear histone H1 had been identified as an immunosuppressive factor in a rat tolerogenic orthotopic liver transplantation (OLT) model. The present study aimed to determine whether the up-regulation of antihistone H1 Ab by histone H1 vaccination l...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2007.01.003

    authors： Nakano T,Goto S,Lai CY,Hsu LW,Ono K,Kawamoto S,Lin YC,Kao YH,Chiang KC,Ohmori N,Goto T,Sato S,Tu CH,Jawan B,Cheng YF,Chen CL

    更新日期：2007-04-01 00:00:00

  • Strategies to achieve immune tolerance in allogeneic solid organ transplantation.

    abstract：:Organ transplantation is an effective way to treat many end-stage diseases. In order to overcome post-transplant rejection, immunosuppressive agents have been widely used, but the long-term survival of transplanted organs still has not been achieved in the clinic. For decades, tolerance is the "holy grail" that transp...

    journal_title：Transplant immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.trim.2019.101250

    authors： Zeng S,Xiao Z,Wang Q,Guo Y,He Y,Zhu Q,Zou Y

    更新日期：2020-02-01 00:00:00

  • Free light chain and intact immunoglobulin abnormalities in heart transplant recipients: Two year follow-up timelines and clinical correlations.

    abstract：OBJECTIVES:To assess the timelines of serum free light chain (sFLC) concentrations and the kappa/lambda light chain (K/L) ratio in heart transplant (HTX) recipients. To analyze the performance of serum protein electrophoresis (SPE), serum immunofixation (sIFE) and sFLC measurements for gammopathy detection following a ...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2017.01.003

    authors： Sečník P Jr,Lavríková P,Hošková L,Malíková P,Jabor A,Franeková J

    更新日期：2017-03-01 00:00:00

  • Preventive effects of syngeneic bone marrow transplantation on diabetic nephropathy in mice.

    abstract：:Treatment with autologous bone marrow transplantation (ABMT) can change the natural history of diabetes in patients with new-onset Type 1 diabetes (T1D). Effects of syngeneic bone marrow transplantation (syn-BMT) on diabetic nephropathy were studied in streptozotocin-induced diabetic mice. Diabetic mice received sibli...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2009.12.003

    authors： Ouyang J,Hu G,Wen Y,Zhang X

    更新日期：2010-02-01 00:00:00

  • Tim-3 expression in human kidney allografts.

    abstract：BACKGROUND:Tim-3 was recently described as a Th1-specific molecule, participating in the regulation of immune responses and in the induction of allograft tolerance. Here, we studied Tim-3 mRNA expression together with molecular markers of T-cell activation and cytotoxicity, in rejected human kidney grafts. METHODS:Twe...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2006.11.003

    authors： Ponciano VC,Renesto PG,Nogueira E,Rangel EB,Cenedeze MA,Franco MF,Câmara NO,Pacheco-Silva A

    更新日期：2007-04-01 00:00:00

  • Evaluation of histological damage of solid organs after donor preconditioning with thymoglobulin in an experimental rat model.

    abstract：:Rabbit anti-rat thymoglobulin (rATG) administered to donors with brain death (BD) may improve organs quality. We explored the effects of rATG administered to BD donors in the histology of heart, lungs and small bowel in a rat experimental model. Animals were randomly assigned to 3 groups: V (n=5) no BD, 2h ventilation...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2013.04.002

    authors： Cicora F,Stringa P,Guerrieri D,Vásquez D,Toniolo F,Roberti J,Raimondi C

    更新日期：2013-06-01 00:00:00

  • Alpha-galactosyl antibody redistributes alpha-galactosyl at the surface of pig blood and endothelial cells.

    abstract：:The interaction of antibodies with cell surface antigens may induce redistribution of immune complexes, followed by antigen depletion, with increased resistance to injurious effect of antibody and complement (antigenic modulation). Human natural antibodies to Gal alpha 1,3Gal beta 1,4GlcNAc-R (alpha Gal) epitopes expr...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/s0966-3274(99)80026-4

    authors： Maruyama S,Cantu E3rd,Pernis B,Galili U,Godman G,Stern DM,Andres G

    更新日期：1999-06-01 00:00:00

  • Impact of pretransplant exposure to allosensitization factors generating HLA antibodies in the Luminex era.

    abstract：AIM:To identify the frequency of exposure to sensitizing factors and evaluate the risk ascribable to each sensitizing factor generating HLAabs measured by Luminex. METHODS:This is a retrospective cohort study that included 502 transplanted patients and 51 patients on the waiting list for a deceased donor graft. Patien...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2016.08.003

    authors： Guichard-Romero A,Marino-Vazquez LA,Castelán N,López M,González-Tableros N,Arvizu A,De Santiago A,Alberú J,Morales-Buenrostro LE

    更新日期：2016-09-01 00:00:00

  • Screening of mortality in transplant patients using an assay for immune function.

    abstract：BACKGROUND:So far, the ImmuKnow Immune Cell Function Assay (Cylex, Inc., Columbia, MD, USA) has been used to assess risks of infection and rejection in transplant patients. We hypothesized that the ImmuKnow assay might be used for mortality screening in transplant patients overall. METHODS:In the period of February 20...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/j.trim.2010.12.005

    authors： Berglund D,Bengtsson M,Biglarnia A,Berglund E,Yamamoto S,von Zur-Mühlen B,Lorant T,Tufveson G

    更新日期：2011-05-01 00:00:00

  • Combined FTY720/cyclosporine A treatment promotes graft survival and lowers the peripheral lymphocyte count in DA to lewis heart and skin transplantation models.

    abstract：OBJECTIVE:The immunomodulator, FTY720, lowers the peripheral lymphocyte count (PLC) by inducing migration of circulating lymphocytes to secondary lymphoid organs. We investigated the efficacy of mono- vs. combined-FTY720/CsA therapy on graft survival (GS) and on lowering the PLC in a solid organ and a skin graft model,...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/s0966-3274(01)00031-4

    authors： Nikolova Z,Hof A,Baumlin Y,Hof RP

    更新日期：2001-02-01 00:00:00

  • High-dose tacrolimus and lengthy survival of the combined rat pancreas/spleen graft in a high-responder combination.

    abstract：:Pancreas grafts do not survive for lengthy periods, especially in a high-responder rat combination. Recent data indicated that a combined spleen/pancreas graft protects against acute graft rejection and induces donor-specific tolerance. In this study, we performed a combination spleen/pancreas transplantation using hi...

    journal_title：Transplant immunology

    pub_type： 杂志文章

    doi：10.1016/s0966-3274(01)00049-1

    authors： Sakuma Y,Uchida H,Nagai H,Kobayashi E

    更新日期：2001-10-01 00:00:00