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T and B cell crossmatching using three-colour flow cytometry.

Abstract：

:We have developed a highly sensitive method for the simultaneous crossmatching of T and B cells using three-colour flow cytometry. This method identifies T and B cell populations in a lymphocyte sample and analyses each for the presence of bound patient IgG alloantibodies. A fluorescein isothiocyanate conjugated rabbit anti-human IgG antibody was used to detect bound IgG following incubation of donor lymphocytes with patient serum. R-phycoerythrin conjugated anti-human CD19, and Quantum Red conjugated anti-human CD3 monoclonal antibodies were used to detect T and B cell populations, respectively. A comparison of results between conventional and three-colour flow cytometry crossmatches showed that there was no difference in detection of bound IgG between the two methods for ten test sera, doubling dilutions of a positive control serum and 72 donor-recipient pairs. Three-colour flow cytometry has proved particularly helpful in determining whether an antibody is directed against donor T and/or B cells and was shown to be more sensitive than complement-dependent cytotoxicity assay in eight out of 72 crossmatches.

journal_name

Transpl Immunol

journal_title

Transplant immunology

authors

Robson A,Martin S

doi

10.1016/s0966-3274(96)80018-9

subject

Has Abstract

pub_date

1996-09-01 00:00:00

pages

203-8

issue

3

eissn

0966-3274

issn

1878-5492

pii

S0966-3274(96)80018-9

journal_volume

4

pub_type

杂志文章

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