Abstract：
:The gene expression of the cytotoxic T-cell molecules perforin, granzyme B and Fas ligand are associated with acute rejection in renal allograft recipients. Several immune mechanisms are linked to severe systemic inflammation in brain-dead organ donors. We examined the mRNA expression of these T-cell activation biomarkers in donor kidney biopsies to evaluate if they could separate living from deceased donors and primary graft function from delayed graft function or acute rejection in the early post transplantation period. We obtained 139 cadaveric and 19 living donor kidney core biopsies post reperfusion and 78 renal allograft biopsies taken because of graft dysfunction. RNA was isolated from tissue samples and mRNA encoding perforin, granzyme B or Fas ligand and a constitutively expressed cyclophilin B, a reference gene, was measured with the use of real-time quantitative polymerase chain reaction assay, and the levels of expression was correlated with allograft status. We did not find statistically significant differences in gene expression of perforin, granzyme B or Fas ligand among deceased and living donor kidneys and the mRNA expression of these cytotoxic molecules in donor kidney biopsies did not distinguish primary allograft function or early acute rejection. Significant differences were found between acute rejection (n=17) and zero-hour samples and acute rejection and non-rejection (n=41) samples for all 3 measured transcripts. No significant difference was found between acute borderline rejection (n=16) and non-rejection samples. In conclusion, effector molecules secreted by cytotoxic T lymphocytes were not activated in deceased donor kidneys and the genes did not classify the post-transplant course.
journal_name
Transpl Immunoljournal_title
Transplant immunologyauthors
Carstens J,Ozbay A,Tørring C,Hansen HEdoi
10.1016/j.trim.2008.12.002subject
Has Abstractpub_date
2009-03-01 00:00:00pages
212-7issue
4eissn
0966-3274issn
1878-5492pii
S0966-3274(08)00126-3journal_volume
20pub_type
杂志文章abstract：:Dendritic cells (DC) are considered to be the major cell type responsible for induction of primary immune responses. While they have been shown to play a critical role in eliciting allosensitization via the direct pathway, there is evidence that maturational and/or activational heterogeneity between DC in different do...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(00)00010-1
更新日期：2000-03-01 00:00:00
abstract：:Solid organ transplant recipients (SOTRs) are susceptible to various cutaneous side effects as a consequence of long-term immunosuppressive therapy. Skin cancers and infections are well-studied complications that can cause death and/or allograft rejection. Other cutaneous drug reactions, such as inflammatory manifesta...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2020.101355
更新日期：2021-02-01 00:00:00
abstract：:Data binding the expression of Toll-like 4 receptor (TLR4ex), transplanted kidney function, and the cause of pre-transplant end-stage renal disease are scarcely available. OBJECTIVE:To investigate the relationship between pre-transplant chronic interstitial nephritis (CIN), TLR4ex and transplanted kidney function. M...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2020.101319
更新日期：2020-10-01 00:00:00
abstract：:The containment of direct and indirect recognition of donor alloantigens by enhancement of the number and activity of regulatory T cells (Tregs) has been one of the promising approaches to achieve transplant tolerance. Two major methods, dendritic cell (DC) and anti-CD3/CD28 antibodies (Abs) have been introduced for e...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2009.07.004
更新日期：2009-12-01 00:00:00
abstract：:We investigated the dynamics of the CD4+ and CD8+ lymphocyte subsets, and the expression of activation markers in cardiac transplant recipients. We tested 132 peripheral blood samples from 62 cardiac transplant recipients using fluorescent staining and flow cytometry analysis. The results were correlated with histolog...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(02)00072-2
更新日期：2002-11-01 00:00:00
abstract：:Application of mesenchymal stromal cells (MSCs) has been proposed for solid organ transplantation based on their potent immuno-modulatory effects in vitro and in vivo. We investigated the potential of MSCs to improve acceptance of kidney transplants in an MHC-incompatible rat model including isogeneic kidney transplan...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2013.08.004
更新日期：2013-12-01 00:00:00
abstract：:We investigated the immunosuppressive effects of the dihydroortate dehydrogenase (DHODH) inhibitor compounds ABR-222417 and ABR-224050 from Active Biotech (Sweden). We verified the inhibitory effects of these compounds on the proliferation of CD4(+) and CD8(+) T-cells in vivo by using superantigen staphylococcus enter...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2010.05.005
更新日期：2010-08-01 00:00:00
abstract：:Calcineurin a calmodulin-dependent phosphatase plays a critical role in calcium-dependent activation of T-lymphocytes and is the major target for the inhibitory actions of the immunosuppressive drugs Tacrolimus (FK506) and Cyclosporin A (CsA). Calcineurin is a dimeric protein consisting of distinct A (catalytic) and B...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(00)00020-4
更新日期：2000-06-01 00:00:00
abstract：:The interaction of antibodies with cell surface antigens may induce redistribution of immune complexes, followed by antigen depletion, with increased resistance to injurious effect of antibody and complement (antigenic modulation). Human natural antibodies to Gal alpha 1,3Gal beta 1,4GlcNAc-R (alpha Gal) epitopes expr...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(99)80026-4
更新日期：1999-06-01 00:00:00
abstract：:New immunosuppressive strategies have improved short- and long-term graft survival. The current aim is to decrease the intensity of the immunosuppressive regimen, in an attempt to limit side effects and the direct toxicity of calcineurin inhibitor (CNI) for kidney function. We describe here current experience in liver...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/j.trim.2006.11.007
更新日期：2007-02-01 00:00:00
abstract：:Previous work on the facilitation of xenogeneic and allogeneic bone marrow engraftment in irradiated mice and dogs with transferrins allowed the development of a model for induction of an apparently durable state of immunological unresponsiveness or 'tolerance' in chemically immunosuppressed mice. The system is based ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(96)80051-7
更新日期：1996-12-01 00:00:00
abstract：:Allospecific memory T cells are a recognized threat to the maintenance of solid-organ transplants. Limited information exists regarding the development of alloreactive memory T cells when post-transplant immunosuppression is present. The clinical practice of delaying calcineurin inhibitor (CNI) initiation post-transpl...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2014.10.001
更新日期：2015-01-01 00:00:00
abstract：:Tissue eosinophils have been previously implicated in allograft rejection and graft loss. The aim of this study was to evaluate the role of eosinophils in acute renal allograft rejection. Data from 71 patients with 114 renal biopsies with acute allograft rejection were compared with those from 26 controls. The median ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/0966-3274(94)90076-0
更新日期：1994-01-01 00:00:00
abstract：:Graft rejection and graft-versus-host disease are leading causes of transplant related mortality despite advancements in immunosuppressive therapy. Mesenchymal stem cells (MSCs) offer a promising addition to immunosuppressive drugs (ISD), while NK-cells are increasingly used as effector cells in graft-versus-leukemia....
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2016.04.008
更新日期：2016-07-01 00:00:00
abstract：:Mycophenolate mofetil (MMF) a potent immunosuppressive agent, has recently been approved for clinical use (CellCept) in renal transplant patients in combination with cyclosporine (CsA). With the expanded use of tacrolimus (Prograf) as well in renal transplant patients, there is a lack of pharmacokinetic studies clarif...
journal_title：Transplant immunology
pub_type： 临床试验,杂志文章
doi：10.1016/s0966-3274(97)80042-1
更新日期：1997-09-01 00:00:00
abstract：:B(io) B(reedding)/O(ttawa) K(alsburg) rats spontaneously develop insulin-dependent type 1 diabetes. Days before BB/OK rats become diabetic, their body seems to be flabby which may be attributed to loss of subcutaneous fat. However, the rats are normoglycemic and manifest 3-4 days later. This observation prompted us to...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2011.01.003
更新日期：2011-05-01 00:00:00
abstract：:Immunological monitoring of transplant recipients is an attractive concept. Cytokines provide an obvious focus for research, as they are central to the human immune response. This study aimed to identify cytokines whose sequential gene expression differentiated rejectors from non-rejectors immediately following renal ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2005.02.003
更新日期：2005-06-01 00:00:00
abstract：BACKGROUND:Mesenchymal stem cells (MSCs) can be used for immunomodulation therapy after solid organ transplantation. Here, we focus on the immunoregulatory potential of combination therapies of MSCs and classic pharmacotherapy to mediate acceptance of solid organ grafts. METHODS:To determine which drugs influence the ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2011.06.002
更新日期：2011-09-01 00:00:00
abstract：:Adenoviral gene transfer is a potential ex vivo gene therapy for islet transplantation. However, the immunogenicity of adenoviral vectors can potentially impair vector efficacy in transplants where long-term gene expression is required. We investigated the effect of this antiviral immunity on vector expression and isl...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2009.02.001
更新日期：2009-05-01 00:00:00
abstract：:TIRC7 delivers essential signals during immune activation as antibodies targeting TIRC7 inhibit lymphocyte proliferation and Th1 cytokine expression in vitro and prolonged kidney and heart allograft survival in vivo. Immunohistochemical analysis of biopsy specimens from human renal allografts undergoing rejection desp...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2006.09.027
更新日期：2006-11-01 00:00:00
abstract：:Previously, we established a murine model, that involves the engraftment of fully allogeneic T cell depleted donor bone marrow cells in sublethally irradiated and single dose anti-CD3 treated recipient mice. These mice developed permanent stable multilineage mixed chimerism and donor-specific tolerance without graft-v...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/S0966-3274(02)00157-0
更新日期：2003-07-01 00:00:00
abstract：:Circulating host lymphocytes recognize, bind to, become activated by, and infiltrate engrafted allogeneic tissues. The mechanisms responsible for these early events which lead to acute immunological rejection have not been precisely defined. We have examined sequentially in vitro lymphocyte binding patterns in a kidne...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/0966-3274(95)80035-2
更新日期：1995-06-01 00:00:00
abstract：BACKGROUND:The Fischer-to-Lewis (LEW) rat model of kidney transplantation is a widely accepted and well-characterized model of chronic rejection. In contrast to transplantation in a clinical setting, however, the absence of treatment with immunosuppressants and only minor mismatch of major histocompatibility complexes ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2016.08.002
更新日期：2016-09-01 00:00:00
abstract：BACKGROUND:Cytomegalovirus (CMV)-specific T-cells are crucial to prevent CMV disease. CMV seropositive recipients transplanted with stem cells from a CMV seronegative allogeneic donor (R+D-) may be at risk for CMV disease due to absence of donor CMV-specific memory T-cells in the graft. METHODS:We analyzed the duratio...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2018.04.003
更新日期：2018-08-01 00:00:00
abstract：:HMG-CoA reductase inhibitors, or statins, are effective lipid lowering agents, extensively used in medical practice. Statins have never been shown to be involved in the immune response, although few clinical reports have suggested a better outcome of cardiac transplantation in patients under pravastatin therapy. Major...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/s0966-3274(02)00030-8
更新日期：2002-05-01 00:00:00
abstract：:Immunisation against Human Leucocyte Antigens (HLA) can be caused by pregnancy, blood transfusion, or organ transplants. The HLA antibody status of a given patient significantly influences their access and waiting time to transplant. For some highly sensitised patients (HSP) there is hardly any suitable donor availabl...
journal_title：Transplant immunology
pub_type： 社论
doi：10.1016/j.trim.2020.101354
更新日期：2021-02-01 00:00:00
abstract：OBJECTIVE:Acute rejection is a major cause of morbidity and mortality after solid organ transplantation. Therefore, optimizing treatment strategies and improving curative effect is urgent and necessary. Reliable biomarkers for acute rejection and the underlying molecular mechanisms remain to be determined. METHODS:In ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2020.101320
更新日期：2020-10-01 00:00:00
abstract：BACKGROUND:So far, the ImmuKnow Immune Cell Function Assay (Cylex, Inc., Columbia, MD, USA) has been used to assess risks of infection and rejection in transplant patients. We hypothesized that the ImmuKnow assay might be used for mortality screening in transplant patients overall. METHODS:In the period of February 20...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2010.12.005
更新日期：2011-05-01 00:00:00
abstract：BACKGROUND:Acute rejection is a major obstacle in patients with prolonged ischemia in deceased-donor renal transplantation. Chemokines and their receptors play a critical role in leukocyte trafficking, resulting in allograft rejection; therefore, the role of chemokine receptor CXCR3 in acute rejection induced by prolon...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2015.08.001
更新日期：2015-10-01 00:00:00
abstract：:Clinical ophthalmologists are highly successful in grafting allogeneic corneas onto healthy eye graft beds--only a small percentage are rejected. By contrast, a high percentage of allografts are rejected when grafted into vascularized or '"high-risk' recipient eyes. The following experiments test the hypothesis that d...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(96)80034-7
更新日期：1996-03-01 00:00:00
