Interleukin-17 immunity in pediatric Crohn disease and ulcerative colitis.

Abstract:

OBJECTIVE:The present understanding of inflammatory bowel disease pathogenesis mainly relies on studies of adult patients. Therefore, we studied the balance between T-effector and regulatory cells in pediatric inflammatory bowel disease. METHODS:Quantitative polymerase chain reaction and immunohistochemistry served to quantify the expression of immunological markers in mucosal biopsies and flow cytometry analysis was used in peripheral blood mononuclear cells. RESULTS:Colonic interleukin (IL)-17+, IL-22, and IL-6 mRNA upregulation and increase in the number of colonic IL-17 cells were demonstrated in both Crohn disease (CD) and ulcerative colitis (UC). Likewise, colonic forkhead box P3 (FOXP3+) mRNA expression and the number of colonic FOXP3 cells were increased both in CD and in UC and were accompanied in CD also with increased numbers of FOXP3+CD25 High CD4 cells in peripheral blood. Ileal relation of IL-17/CD4 cells was increased only in CD. CONCLUSIONS:We showed activation of colonic IL-17/IL-22 axis and upregulation of FOXP3 to occur both in pediatric CD and in UC, indicating shared immunological characteristics. Upregulation of IL-17 was restricted to colon in UC, but existed in the ileum and in the colon in active CD.

authors

Hölttä V,Klemetti P,Salo HM,Koivusalo A,Pakarinen M,Westerholm-Ormio M,Kolho KL,Vaarala O

doi

10.1097/MPG.0b013e3182979252

subject

Has Abstract

pub_date

2013-09-01 00:00:00

pages

287-92

issue

3

eissn

0277-2116

issn

1536-4801

pii

00005176-201309000-00007

journal_volume

57

pub_type

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