Prenatal lipopolysaccharide increases postnatal intestinal injury in a rat model of necrotizing enterocolitis.

Abstract:

BACKGROUND:An increased incidence of necrotizing enterocolitis (NEC) has been noted in infants who are born to mothers with chorioamnionitis. HYPOTHESIS:Our objective was to test the hypothesis that newborn rat pups born to mothers exposed to prenatal lipopolysaccharide during pregnancy would be more susceptible to intestinal injury in a rat model of NEC and that the increased intestinal injury is mediated by dysregulation of inducible nitric oxide synthase. METHODS:Time-dated pregnant Sprague-Dawley dams were given an intraperitoneal injection of either 2 mg/kg of lipopolysaccharide or vehicle. Rat pups from each group of dams were delivered at term and placed in a rat NEC model. A subset of pups was given either vehicle or aminoguanidine. Intestines were harvested and graded for degree of intestinal injury. RESULTS:Maternal prenatal lipopolysaccharide exposure increased the frequency and severity of intestinal injury in the neonatal rat NEC model. Treatment with aminoguanidine significantly decreased plasma nitric oxide levels. Additionally, aminoguanidine significantly decreased intestinal injury. CONCLUSIONS:Intestinal injury observed may be mediated via nitric oxide synthase dysregulation.

authors

Giannone PJ,Nankervis CA,Richter JM,Schanbacher BL,Reber KM

doi

10.1097/mpg.0b013e31818936b8

subject

Has Abstract

pub_date

2009-03-01 00:00:00

pages

276-82

issue

3

eissn

0277-2116

issn

1536-4801

pii

00005176-200903000-00009

journal_volume

48

pub_type

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