Abstract:
:We recently suggested that peroxisome proliferators (PP)-induced hepatocyte DNA synthesis may be mediated by a specific peroxisome proliferator activated receptor (PPAR). Heterodimers of the PPAR with the retinoid nuclear receptor, RXR, activate transcription after binding to DR1 response elements of the target genes. DR1 elements are also activated by RXR homodimers formed in the presence of 9-cis retinoic acid (9 cis RA) suggesting that PP and 9 cis RA might regulate an overlapping set of target genes. The present study was therefore designed to test whether 9-cis RA stimulates hepatocyte DNA synthesis. Male Wistar rats were given a single intragastric dose of 9-cis RA (10-100 mg/Kg) or all trans retinoic acid (RA)(200 mg/Kg and 100 mg/Kg), and levels of hepatocyte DNA synthesis after 24 hours were determined by BrdU immunohistochemistry. Effects of 9-cis RA and RA(10(-9)-10(-5)M) on hepatocyte DNA synthesis in primary culture were also examined. Over 10 fold increases in the levels of BrdU incorporation were noted 24 hours after a single dose of 9 cis RA at a dose of 60 and 100 mg/Kg. RA at a dose of 200 mg/Kg induced a 5-6 fold increases in BrdU labeling, while a dose of 100 mg/Kg had no significant effects. Since the RA effect only occurs at higher doses, it may be only after conversion to 9-cis RA. In primary culture of hepatocytes, neither 9-cis RA nor RA with or without EGF had stimulatory effects on hepatocyte DNA synthesis. This is the first report to demonstrate a potent stimulatory effect of 9-cis RA on DNA synthesis of rat hepatocytes in vivo. It is suggested that 9-cis RA exerts this effect through receptor mediated mechanisms similar to PP, both activating genes that regulate hepatocyte proliferation.
journal_name
Life Scijournal_title
Life sciencesauthors
Ohmura T,Columbano GL,Columbano A,Katyal SL,Locker J,Shinozuka Hdoi
10.1016/0024-3205(96)00039-2subject
Has Abstractpub_date
1996-01-01 00:00:00pages
PL211-216issue
11eissn
0024-3205issn
1879-0631pii
0024320596000392journal_volume
58pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::We administered intravenous adenosine to 11 neonatal rabbits. Adenosine depressed respiration in 10 of 11 rabbits. For the group as a whole the adenosine-induced respiratory depression was highly significant (p less than 0.001). After aminophylline administration to the same animals the respiratory effect of intraveno...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90248-7
更新日期:1987-01-05 00:00:00
abstract:AIMS:The objective of this study is to investigate glucosamine (GlcN) as a transcriptional regulator of iNOS and other genes in association with the dynamic O-GlcNAcylation of RNA polymerase II (RNAPII). MAIN METHODS:The LPS- and/or GlcN-stimulated transcriptional activities of various Gal4-binding site/TATA-box-conta...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.06.011
更新日期:2014-08-21 00:00:00
abstract::Chronic administration of narcotic mu opioid agonists results in tolerance and dependence. We propose that agonist stimulation causes a gradual conversion of mu receptors to a constitutively active state (mu*) as a key step in tolerance and physical dependence. We provide evidence in support of the existence of mu* in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)90022-1
更新日期:1994-01-01 00:00:00
abstract::Human, dog and rabbit corpus cavernosum type 5 phosphodiesterases (PDE5) were isolated and their characteristics were compared. The three enzymes showed Km values of 0.8, 2.1 and 2.3 uM, respectively. They exhibited similar pH-dependence with optimal pH being 7.5. They required Mg++ for activity and the activity was s...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)00989-4
更新日期:2001-03-16 00:00:00
abstract:AIM:Epidermal growth factor receptor (EGFR) related reactive oxygen species (ROS) generation results in myocardial damage. We aimed to investigate the role of gefitinib (EGFR-tyrosine kinase inhibitor) in diabetic cardiomyopathy (DbCM). METHOD:DbCM was induced by injecting streptozotocin (55 mg/kg for 5 consecutive da...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.08.018
更新日期:2018-10-01 00:00:00
abstract:AIMS:Diabetes increases oxidant stress and impairs endothelium-dependent relaxation. We investigated whether the antioxidant 3',4'-dihydroxyflavonol (DiOHF) reduces the release of superoxide (O(2)(-)) and preserves endothelial function in aortae from diabetic rats. MAIN METHODS:Type-1 diabetes was induced in Sprague-D...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.04.012
更新日期:2009-07-03 00:00:00
abstract:AIM:Betel-nut, a popular masticatory among Southeast Asian populations is a class I carcinogen, previously associated with dyslipidemia and aberrant lipid metabolism, and is reported to be used more frequently by females, than males. This study investigates the potential of repurposing the anti-diabetic drug, vildaglip...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118870
更新日期:2021-02-01 00:00:00
abstract:UNLABELLED:The present study was conducted to examine whether melatonin impairs serotonin (5HT) release and uptake in rat platelets. Exposure of platelet-rich plasma samples (PRP) to melatonin induced a concentration-dependent inhibition of 5HT uptake and the value of IC50 was 1.3 x 10(-3) M. We have also investigated ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90261-z
更新日期:1993-01-01 00:00:00
abstract::A modification of beta-adrenoceptor binding has been observed in different human and animal tissues after glucocorticoid administration. In this study we observed that hydrocortisone increased human intact lymphocyte beta-adrenoceptors labelled with 3H-dyhydroalprenolol in vitro, in physiological and pharmacological c...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90528-9
更新日期:1984-12-03 00:00:00
abstract::Glucose-6-phosphate dehydrogenase (G6PD) deficient red blood cells (RBCs) are known to be more susceptible to oxidant-induced hemolysis. Erythrocytes from G6PD-deficient individuals are significantly more susceptible to Ca(2+)-induced vesiculation than normal control cells. The enhanced susceptibility of G6PD-deficien...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00377-3
更新日期:1996-01-01 00:00:00
abstract::Nitric oxide (NO) is a key mediator in many physiological processes and one of the major receptors through which NO exerts its effects is soluble guanylyl cyclase. Guanylyl cyclase converts GTP to cyclic GMP as part of the cascade that results in physiological processes such as smooth muscle relaxation, neurotransmiss...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)02361-5
更新日期:2003-01-17 00:00:00
abstract:AIMS:Active immunization with human vascular endothelial growth factor (hVEGF) vaccines provides a therapeutic option instead of bevacizumab therapy. However, the immunity to self-molecule is difficult to elicit due to immune tolerance. A bioactive peptide of two tandem repeats of mHSP70407-426 (M2) has exhibited poten...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.03.039
更新日期:2018-05-15 00:00:00
abstract::By using multiple time-point constant-rate infusions of deuterium-labeled phosphorylcholine, appropriate kinetic parameters were obtained for use in the calculation of the turnover rate of acetylcholine (TRACh) in selected mouse brain regions. After obtaining these data, the relationship between the analgesic agent co...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90499-f
更新日期:1992-01-01 00:00:00
abstract:AIMS:The study aimed to investigate the protective effect of chronic intermittent hypobaric hypoxia (CIHH) on endothelium function and relaxation of mesenteric artery in metabolism syndrome (MS) rats. MAIN METHODS:Male adult Sprague-Dawley rats were randomly divided into control (CON), CIHH (treated with 28-days hypob...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.05.008
更新日期:2018-07-15 00:00:00
abstract:OBJECTIVE:This study aims to evaluate the effective of azoles and MTX for patients with invasive candidiasis. METHODS:We used the disk diffusion assay and the checkerboard assay to evaluate the in vitro interactions between MTX and antifungals. In addition, we used the transmission electron microscopy to observe the u...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116827
更新日期:2019-10-15 00:00:00
abstract::The role of FSH in inducing folliculogenesis is well established. Recently, the availability of pure FSH has led to a reevaluation of its role in the process of ovulation. Previously, these functions have been examined separately, usually with pregnant mares' serum gonadotropin (PMSG) followed by FSH for ovulation or ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90608-6
更新日期:1993-01-01 00:00:00
abstract::Acute and chronic opiate exposure impairs the emptying of bile from the gallbladder. In this study, the effects of a 4-day morphine regimen on bile composition were examined. Bile acids and phospholipids concentration of bile obtained from the gallbladder of female morphine-treated (MT) guinea pigs were reduced by 60%...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90231-q
更新日期:1993-01-01 00:00:00
abstract::This paper describes a new formulation for triggered delivery of insulin which consisted of magnetic particles dispersed in alginate spheres. When magnetic field was applied to that system, the release rate of insulin was about 50 times higher than in the absence of magnetic field. The influence of oscillating magneti...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90009-4
更新日期:1988-01-01 00:00:00
abstract::Inositol hexaphosphate (InsP6 or IP6) is ubiquitous. At 10 microM to 1 mM concentrations, IP6 and its lower phosphorylated forms (IP(1-5)) as well as inositol (Ins) are contained in most mammalian cells, wherein they are important in regulating vital cellular functions such as signal transduction, cell proliferation a...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/s0024-3205(97)00092-1
更新日期:1997-01-01 00:00:00
abstract::A series of studies were conducted to determine the effects of leucine-(leu-) enkephalin and methionine-(met-) enkephalin on perfusion pressure. These experiments utilized isolated perfused femoral arterial preparations in pentobarbital-anesthetized cats. The enkephalins were administered intraarterially into the femo...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90047-9
更新日期:1982-10-11 00:00:00
abstract::Fibrotic lung diseases qualify among the most dreaded irreversible interstitial pulmonary complications with progressive yet largely unpredictable clinical course. Idiopathic pulmonary fibrosis (IPF) is the most challenging prototype characterized by unknown and complex molecular etiology, severe dearth of non-invasiv...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.118201
更新日期:2020-10-01 00:00:00
abstract::We demonstrate that injections of 1-methyl-4-phenyl-1,2,3,6-tetra-hydropyridine (MPTP), 1-methyl-4-phenyl-pyridinium ion (MPP+) and Paraquat (PQ+) produce in Rana Pipiens different behavioral, biochemical and skin pigmentation changes. MPTP causes in frogs the main symptoms of Parkinsonism (rigidity, akinesia and trem...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90185-7
更新日期:1985-10-21 00:00:00
abstract:OBJECTIVE:We aimed to explore the expression level and biological function of miR-145-5p in preeclampsia (PE). METHODS:The differentially expressed miRNA/mRNA between normal placentas and PE placentas were screened using the GSE84260 and GSE73374 datasets from the Gene Expression Omnibus Database. The expression of mi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.117008
更新日期:2019-12-15 00:00:00
abstract::To elucidate the pharmacological profile of P2X receptors and the probable expression of P2Y receptors in otic ganglion neurons from 17-day-old rats, single neurons were enzymatically isolated and maintained in tissue culture for up to 30 h. Whole-cell voltage-clamp recording was carried out at a holding potential of ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.06.005
更新日期:2008-08-01 00:00:00
abstract::The tumor promoter okadaic acid (OKA), is a marine toxin of algal origin, identified as a potent inhibitor of protein phosphatases 1 and 2A, and possibly enhancing calcium influx through voltage dependent calcium channels (VSSC). We now report that OKA at concentrations as low as 0.5 nM produced neurotoxicity, charact...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90398-u
更新日期:1991-01-01 00:00:00
abstract::Histidine decarboxylase (HDC) activity and histamine content were measured in endoscopic gastric biopsy specimens of 19 control subjects with normogastrinemia and 6 patients with hypergastrinemia. In controls, the HDC activity was 3 fold higher in fundic mucosa (120 +/- 13 fmol/min/mg protein, mean +/- S.E.) than in a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90424-a
更新日期:1991-01-01 00:00:00
abstract::Neurodegenerative diseases need the drugs to be delivered right inside the brain to maximizing the therapeutic effects. This can be achieved by use of novel targeted delivery systems such as nanoemulsions. Nanoemulsions (NE) are nano-sized emulsions that are manufactured for enhancing the delivery of drugs to the targ...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.117394
更新日期:2020-03-15 00:00:00
abstract::Adenylate cyclase activity in isolated rat liver plasma membranes was inhibited by NADH in a concentration-dependent manner. Half-maximal inhibition of adenylate cyclase was observed at 120 microM concentration of NADH. The effect of NADH was specific since adenylate cyclase activity was not altered by NAD+, NADP+, NA...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90177-d
更新日期:1991-01-01 00:00:00
abstract:AIMS:Dorsal root ganglia contain heterogeneous populations of primary afferent neurons that transmit various sensory stimuli. This functional diversity may be correlated with differential expression of voltage-gated K(+) (Kv) channels. Here, we examine cellular distributions of Kv4 pore-forming and ancillary subunits t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.07.007
更新日期:2012-09-17 00:00:00
abstract::Initial acute behavioral studies in mice indicated that phencyclidine (PCP) produced marked motor impairment as measured by the inverted screen technique with an ED50 value of 4.1 muMole/kg (i.v.). Phenylcyclohexene (PC) was considerably less active with an ED50 value of 325 muMole/kg (i.v.). PCP was also shown to be ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90708-1
更新日期:1982-08-23 00:00:00