Abstract：
:In the present work, we have evaluated the effect of an acute addition of melatonin on cholecystokinin octapeptide (CCK-8)-evoked Ca(2+) signals and amylase secretion in mouse pancreatic acinar cells. For this purpose, freshly isolated mouse pancreatic acinar cells were loaded with fura-2 to study intracellular free Ca(2+) concentration ([Ca(2+)](c)). Amylase release and cell viability were studied employing colorimetric methods. Our results show that CCK-8 evoked a biphasic effect on amylase secretion, finding a maximum at a concentration of 0.1 nM and a reduction of secretion at higher concentrations. Pre-incubation of cells with melatonin (1 μM-1 mM) significantly attenuated enzyme secretion in response to high concentrations of CCK-8. Stimulation of cells with 1 nM CCK-8 led to a transient increase in [Ca(2+)](c), followed by a decrease towards a constant level. In the presence of 1 mM melatonin, stimulation of cells with CCK-8 resulted in a smaller [Ca(2+)](c) peak response, a faster rate of decay of [Ca(2+)](c) and lower values for the steady state of [Ca(2+)](c), compared with the effect of CCK-8 alone. Melatonin also reduced the oscillatory pattern of Ca(2+) mobilization evoked by a physiological concentration of CCK-8 (20 pM), and completely inhibited Ca(2+) mobilization induced by 10 pM CCK-8. On the other hand, Ca(2+) entry from the extracellular space was not affected in the presence of melatonin. Finally, melatonin alone did not change cell viability. We conclude that melatonin, at concentrations higher than those found in blood, might regulate exocrine pancreatic function via modulation of Ca(2+) signals.
journal_name
J Physiol Biochemjournal_title
Journal of physiology and biochemistryauthors
Santofimia-Castaño P,Ruy DC,Salido GM,González Adoi
10.1007/s13105-013-0267-2subject
Has Abstractpub_date
2013-12-01 00:00:00pages
897-908issue
4eissn
1138-7548issn
1877-8755journal_volume
69pub_type
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章,评审
doi：10.1007/s13105-013-0265-4
更新日期：2013-09-01 00:00:00
abstract：:There are a number of hypothetical explanations for the actions of ethanol on the exocrine pancreas; among them, the cholinergic hypothesis has received special attention. According to this hypothesis, chronic alcohol consumption induces alterations in the control of exocrine pancreatic function resulting in cholinerg...
journal_title：Journal of physiology and biochemistry
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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更新日期：2018-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：2010-03-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章,评审
doi：10.1007/BF03167033
更新日期：2004-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2003-09-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
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更新日期：2006-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s13105-016-0537-x
更新日期：2017-05-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF03165766
更新日期：2007-12-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章,评审
doi：10.1007/s13105-016-0536-y
更新日期：2017-02-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s13105-011-0087-1
更新日期：2011-09-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF03165804
更新日期：2006-03-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s13105-015-0385-0
更新日期：2015-03-01 00:00:00
abstract：:We and others have demonstrated a protective role for pacing postconditioning (PPC) against ischemia/reperfusion (I/R) injury in the heart; however, the underlying mechanisms behind these protective effects are not completely understood. In this study, we wanted to further characterize PPC-mediated cardiac protection,...
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pub_type： 杂志文章
doi：10.1007/s13105-016-0535-z
更新日期：2017-05-01 00:00:00
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journal_title：Journal of physiology and biochemistry
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更新日期：2014-12-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
