Abstract：
:Activation of 5' adenosine monophosphate-activated protein kinase (AMPK) with aminoimidazole carboxamide ribonucleotide (AICAR) increases skeletal muscle glucose uptake and fatty acid oxidation. The purpose of these experiments was to utilize AICAR to enhance palmitate consumption by mitochondria in cultured skeletal muscle cells. In these experiments, we treated C2C12 myotubes or adult single skeletal muscle fibers with varying concentrations of AICAR for different lengths of time. Surprisingly, acute AICAR exposure at most concentrations (0.25-1.5 mM), but not all (0.1 mM), modestly inhibited oxygen consumption even though AICAR increased AMPK phosphorylation. The data suggest that AICAR inhibited oxygen consumption by the cultured muscle in a non-specific manner. The results of these experiments are expected to provide valuable information to investigators interested in using AICAR in cell culture studies.
journal_name
J Physiol Biochemjournal_title
Journal of physiology and biochemistryauthors
Spangenburg EE,Jackson KC,Schuh RAdoi
10.1007/s13105-013-0269-0subject
Has Abstractpub_date
2013-12-01 00:00:00pages
909-17issue
4eissn
1138-7548issn
1877-8755journal_volume
69pub_type
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pub_type： 杂志文章
doi：
更新日期：1998-06-01 00:00:00
