Abstract:
:We and others have demonstrated a protective role for pacing postconditioning (PPC) against ischemia/reperfusion (I/R) injury in the heart; however, the underlying mechanisms behind these protective effects are not completely understood. In this study, we wanted to further characterize PPC-mediated cardiac protection, specifically identify optimal pacing sites; examine the role of oxidative stress; and test the existence of a potential synergistic effect between PPC and adenosine. Isolated rat hearts were subjected to coronary occlusion followed by reperfusion. PPC involved three, 30 s, episodes of alternating left ventricular (LV) and right atrial (RA) pacing. Multiple pacing protocols with different pacing electrode locations were used. To test the involvement of oxidative stress, target-specific agonists or antagonists were infused at the beginning of reperfusion. Hemodynamic data were digitally recorded, and cardiac enzymes, oxidant, and antioxidant status were chemically measured. Pacing at the LV or RV but not at the heart apex or base significantly (P < 0.001) protected against ischemia-reperfusion injury. PPC-mediated protection was completely abrogated in the presence of reactive oxygen species (ROS) scavenger, ebselen; peroxynitrite (ONOO-) scavenger, uric acid; and nitric oxide synthase inhibitor, L-NAME. Nitric oxide (NO) donor, snap, however significantly (P < 0.05) protected the heart against I/R injury in the absence of PPC. The protective effects of PPC were significantly improved by adenosine. PPC-stimulated protection can be achieved by alternating LV and RA pacing applied at the beginning of reperfusion. NO, ROS, and the product of their interaction ONOO- play a significant role in PPC-induced cardiac protection. Finally, the protective effects of PPC can be synergized with adenosine.
journal_name
J Physiol Biochemjournal_title
Journal of physiology and biochemistryauthors
Babiker FA,Al-Jarallah A,Joseph Sdoi
10.1007/s13105-016-0535-zsubject
Has Abstractpub_date
2017-05-01 00:00:00pages
175-185issue
2eissn
1138-7548issn
1877-8755pii
10.1007/s13105-016-0535-zjournal_volume
73pub_type
杂志文章abstract::MicroRNAs (miRNAs), a class of small, single-stranded endogenous RNAs, act as post-transcriptional regulators of gene expression. The ability of one single miRNA regulating multiple functionally related mRNAs makes it a new potential candidate for cancer gene therapy. Let-7s miRNAs have been demonstrated as tumor-supp...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-011-0124-0
更新日期:2012-03-01 00:00:00
abstract::Increased incidence of chronic kidney disease (CKD) with consecutive progression to end-stage renal disease represents a significant burden to healthcare systems. Renal tubulointerstitial fibrosis (TIF) is a classical hallmark of CKD and is well correlated with the loss of renal function. The bioactive lysophospholipi...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-016-0518-0
更新日期:2016-12-01 00:00:00
abstract::Human leukocyte antigen (HLA)-G is an immunomodulatory molecule discovered for the first time in the maternal-fetal interface. In cancer context, where high number of natural killer (NK) cells is described, the presence of HLA-G in its soluble form is thought to be essential for NK cells signaling. To evaluate intrace...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-010-0005-y
更新日期:2010-03-01 00:00:00
abstract::In order to evaluate the expression of nuclear receptors at the peripheral level in obese subjects, messenger RNA (mRNA) levels of different isoforms of retinoic acid receptor (RAR), triiodothyronine (TR), and peroxisome proliferator-activated receptor (PPAR) were determined and compared in peripheral mononuclear bloo...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-010-0006-x
更新日期:2010-03-01 00:00:00
abstract::Decline in oxygen availability experienced under hypobaric hypoxia (HH) mediates imbalance in lung fluid clearance and is a causative agent of acute lung injury. Here, we investigate the pathological events behind acute HH mediated lung injury and assess the therapeutic efficacy of nanocurcumin in its amelioration. We...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-016-0515-3
更新日期:2016-12-01 00:00:00
abstract::In pregnancy there occurs maternal tolerance to the foetus. Several mechanisms have been proposed to explain this phenomenon. The main immune population in the decidua are macrophages and natural killer cells, but with some "special" suppressor characteristics. There is also a predominant TH2 response. The non classic...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章,评审
doi:10.1007/BF03167033
更新日期:2004-09-01 00:00:00
abstract::While carnitine overload appears to have therapeutic effects in pathological situations such as heart recovery after ischemia, its benefits as dietary supplementation for aerobic exercise have been questioned. We studied the effect of carnitine supplementation on the response of perfused rat heart to ischemia and repe...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF03168229
更新日期:2008-03-01 00:00:00
abstract::Although the human homologue of SND p102, p100 coactivator, was initially described as a nuclear protein, the p100 coactivator protein family members have non-nuclear localization in mammalian cells with active lipid handling, storage, and secretion. However, their role in lipid homeostasis remains unresolved. Here, w...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-010-0011-0
更新日期:2010-03-01 00:00:00
abstract::Skeletal muscle atrophy commonly occurs during ageing, thus pathways that regulate muscle mass may represent a potential therapeutic avenue for interventions. In this review, we explored the Hippo signalling pathway which plays an essential role in human oncogenesis and the pathway's influence on myogenesis and satell...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章,评审
doi:10.1007/s13105-021-00787-z
更新日期:2021-01-26 00:00:00
abstract::The effect of long-lasting in vivo restriction of nitric oxide (NO) bioavailability on cardiac and renal P-type ATPases critical for intracellular ion homeostasis is controversial. Previous work has shown in eNOS knockout (eNOS(-/-)) mice hearts that Na(+)/K(+)- and Ca(2+)-ATPase activities were depressed but the unde...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-012-0203-x
更新日期:2013-06-01 00:00:00
abstract::Diabetes is a risk factor for cardiovascular disease that has a multifactorial etiology, with oxidative stress as an important component. Our previous observation of a significant diabetes-related increase in rat cardiac catalase (CAT) activity suggested that CAT could play a major role in delaying the development of ...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-014-0363-y
更新日期:2014-12-01 00:00:00
abstract::Adiponectin, a hormone produced by adipocytes, is involved in glucose metabolism and insulin sensitivity. The production of this adipokine is impaired in obesity and insulin resistance. Eicosapentaenoic acid (EPA) is a dietary n-3 polyunsaturated fatty acid that improves insulin sensitivity in several models of obesit...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF03174067
更新日期:2006-06-01 00:00:00
abstract::The number of patients that suffer some type of spinal cord lesion in recent years are high and have increased because of factors such as traffic accidents. Although their life expectancy has increased, cardiovascular illnesses is one of the main causes of morbidity and mortality. Since the degree of physical fitness ...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF03179863
更新日期:2003-03-01 00:00:00
abstract::In spite of a wide range of drugs being available in the market, treatment of arterial hypertension still remains a challenge, and new therapeutic strategies could be developed in order to improve the rate of success in controlling this disease. Since oxidative stress has gained importance in the last few years as one...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-010-0017-7
更新日期:2010-06-01 00:00:00
abstract::The present study was addressed to determine whether the high expression of peroxisome proliferator-activated receptor-alpha (PPAR-alpha) in rat liver during the perinatal stage plays a role in the induction of liver lipoprotein lipase (LPL) expression and activity. Parallel increases in liver mRNA PPAR-alpha and LPL ...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF03168468
更新日期:2006-09-01 00:00:00
abstract::O-Acetylserine(thiol)lyase (OASTL) enzyme catalyzes the cysteine biosynthesis in photosynthetic organisms. In mature Arabidopsis thaliana the highest activity level is observed in non-photosynthetic tissues like roots, that also show significant amount of protein detected by Western blot analysis. By means of specific...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::Serotonin (5-HT) is one of the regulators of feeding in humans. Drugs acting on the serotoninergic system are used to treat bulimia nervosa and to enhance the effect of hypocaloric diets in overweight subjects. They act rapidly to normalise feeding when used to treat eating-related problems. To explore the role of the...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-012-0188-5
更新日期:2013-03-01 00:00:00
abstract::There are a number of hypothetical explanations for the actions of ethanol on the exocrine pancreas; among them, the cholinergic hypothesis has received special attention. According to this hypothesis, chronic alcohol consumption induces alterations in the control of exocrine pancreatic function resulting in cholinerg...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF03179781
更新日期:2000-09-01 00:00:00
abstract::The weight loss observed in consumers of extracts of Citrus aurantium (bitter orange) has been tentatively attributed to the lipolytic and thermogenic effects of the alkaloids abundant in the unripe fruit. Synephrine, octopamine, tyramine, and other alkaloids have been repeatedly identified and quantified in Citrus me...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-011-0078-2
更新日期:2011-09-01 00:00:00
abstract::Gut bacteria play an important role in several metabolic processes and human diseases, such as obesity and accompanying co-morbidities, such as fatty liver disease, insulin resistance/diabetes, and cardiovascular events. Among other factors, dietary patterns, probiotics, prebiotics, synbiotics, antibiotics, and non-di...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章,评审
doi:10.1007/s13105-017-0564-2
更新日期:2018-02-01 00:00:00
abstract::Cholesterolemia is associated with pro-oxidative and proinflammatory effects. Glucomannan- or glucomannan plus spirulina-enriched surimis were included in cholesterol-enriched high-saturated diets to test the effects on lipemia; antioxidant status (glutathione status, and antioxidant enzymatic levels, expressions and ...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-015-0425-9
更新日期:2015-12-01 00:00:00
abstract::The study investigates the effect of weight-maintaining high-sucrose (HSD) and high-fat (HFD) diets on plasma glucose and insulin concentrations in lean and obese women, and verifies the correlation between insulin profile and body composition. Lean (G1 group, n=6, BMI= 21.4 (20.2-22.8) kg/m2) and overweight/obese (G2...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF03168238
更新日期:2008-06-01 00:00:00
abstract::Vitamin A or retinol plays a major role in the regulation of cellular homeostasis. Retinyl palmitate remains the main chemical form of vitamin A storage and is mainly located in hepatic stellate cells (HSCs) in lipid droplets resembling those found in adipose cells. White adipose tissue (WAT), is essentially involved ...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章,评审
doi:10.1007/s13105-011-0101-7
更新日期:2011-09-01 00:00:00
abstract::Currently, there are no reports in the literature demonstrating any animal model that ingests one of the fattiest animal food source, the bovine brain. We hypothesized that a high-fat diet (HFD), based on dried bovine brain, could be used to develop an animal model possessing a spectrum of insulin resistance-related f...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-011-0085-3
更新日期:2011-09-01 00:00:00
abstract::While the unequivocal pattern of endothelial nitric oxide synthase (eNOS) inhibition in cardiovascular control is recognized, the role of NO produced by neuronal NOS (nNOS) remains unclear. The aim of this study was to compare the effects of chronic treatment with 7-nitroindazole (7-NI, nNOS inhibitor) and N(G)-nitro-...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-014-0343-2
更新日期:2014-09-01 00:00:00
abstract::This study was designed to investigate effects of raloxifene (RLX) and estradiol on bone formation and resorption in intact and ovariectomized (ovx) rat models. In the intact model, a total of 24 adult female rats were divided into three groups: Controls subcutaneously received saline alone. RLX (2 mg/kg) and estradio...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-010-0008-8
更新日期:2010-03-01 00:00:00
abstract::Low shear stress (LSS) occurs in areas where atherosclerosis is prevalent. Many studies have revealed that signal transducer and activator of transcription 1 (STAT1) plays a significant role in cardiovascular disease. Nonetheless, the mechanism underlying the connection between STAT1 and LSS is not fully understood. T...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-020-00729-1
更新日期:2020-02-01 00:00:00
abstract::Type 2 diabetes (T2D) is a complicated systemic disease, and the exact pathogenetic molecular mechanism is unclear. Distinct histone modifications regulate gene expression in certain diseases, but little is known about histone epigenetics in diabetes. In the current study, C57BL/6 J mice were used to build T2D model, ...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-015-0385-0
更新日期:2015-03-01 00:00:00
abstract::High plasma lactate levels have been associated with reduced mitochondrial respiratory capacity and increased type 2 diabetes risk, while mitochondrial DNA (mtDNA) copy number has been proposed as a biomarker of mitochondrial function linked to glucose homeostasis. The aim of this study was to evaluate the association...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s13105-019-00672-w
更新日期:2019-08-01 00:00:00
abstract::In response to stress, the hypothalamus releases cortiticotropin releasing hormone (CRH) that travels to the anterior pituitary, where it stimulates the release of adrenocorticotropic hormone (ACTH). ACTH travels to the adrenal cortex, where it stimulates the release of cortisol and other steroids that liberate energy...
journal_title:Journal of physiology and biochemistry
pub_type: 杂志文章,评审
doi:10.1007/s13105-012-0227-2
更新日期:2013-09-01 00:00:00