Isopropylnorsynephrine is a stronger lipolytic agent in human adipocytes than synephrine and other amines present in Citrus aurantium.

Abstract:

:The weight loss observed in consumers of extracts of Citrus aurantium (bitter orange) has been tentatively attributed to the lipolytic and thermogenic effects of the alkaloids abundant in the unripe fruit. Synephrine, octopamine, tyramine, and other alkaloids have been repeatedly identified and quantified in Citrus members of the Rutaceae family or in their extracts incorporated in dietary supplements for weight management. However, there are only scarce reports on their lipolytic action. This study aimed at comparing the acute lipolytic activity of synephrine, octopamine, tyramine, and N-methyltyramine in rat and human adipocytes. Maximal response to the prototypical β-adrenergic agonist isoprenaline was taken as reference in both species. In rat, octopamine was slightly more active than synephrine while tyramine and N-methyl tyramine did not stimulate-and even inhibited-lipolysis. In human adipocytes, none of these amines stimulated lipolysis when tested up to 10 μg/ml. At higher doses (≥100 μg/ml), tyramine and N-methyl tyramine induced only 20% of the maximal lipolysis and exhibited antilipolytic properties. Synephrine and octopamine were partially stimulatory at high doses. Since synephrine is more abundant than octopamine in C. aurantium, it should be the main responsible for the putative lipolytic action of the extracts claimed to mitigate obesity. Noteworthy, their common isopropyl derivative, isopropylnorsynephrine (also named isopropyloctopamine or betaphrine), was clearly lipolytic: active at 1 μg/ml and reproducing more than 60% of isoprenaline maximal effect in human adipocytes. This compound, not detected in C. aurantium, and which has few reported adverse effects to date, might be useful for in vivo triglyceride breakdown.

journal_name

J Physiol Biochem

authors

Mercader J,Wanecq E,Chen J,Carpéné C

doi

10.1007/s13105-011-0078-2

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

443-52

issue

3

eissn

1138-7548

issn

1877-8755

journal_volume

67

pub_type

杂志文章
  • The role of roots in cysteine biosynthesis by Arabidopsis thaliana.

    abstract::O-Acetylserine(thiol)lyase (OASTL) enzyme catalyzes the cysteine biosynthesis in photosynthetic organisms. In mature Arabidopsis thaliana the highest activity level is observed in non-photosynthetic tissues like roots, that also show significant amount of protein detected by Western blot analysis. By means of specific...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:

    authors: Barroso C,Vega JM,Gotor C

    更新日期:1998-12-01 00:00:00

  • Multi-regulatory network of ROS: the interconnection of ROS, PGC-1 alpha, and AMPK-SIRT1 during exercise.

    abstract::Transcriptional factors are easily susceptible to any stimuli, including exercise. Exercise can significantly influence PGC-1 α and AMPK-SIRT1 pathway, as it is involved in the regulation of energy metabolism and mitochondrial biogenesis. Exercise is a major energy deprivation process by which many of transcription fa...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章,评审

    doi:10.1007/s13105-017-0576-y

    authors: Thirupathi A,de Souza CT

    更新日期:2017-11-01 00:00:00

  • Production of healthier goat milk. Use of a concentrate supplemented with a "protected" fat rich in PUFA.

    abstract::In order to obtain healthier goat milk as far as its fat composition is concerned, milk production and composition trials were carried out with Granadina goats kept in semi-extensive breeding conditions. The animals were feeding indoors with a concentrate supplemented or not with 7% of fat conveniently protected again...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/BF03179791

    authors: Sanz Sampelayo MR,Martín Alonso JJ,Morón D,Pérez L,Boza J

    更新日期:2000-09-01 00:00:00

  • The role of GLUT2 in dietary sugar handling.

    abstract::GLUT2 is a facilitative glucose transporter located in the plasma membrane of the liver, pancreatic, intestinal, kidney cells as well as in the portal and the hypothalamus areas. Due to its low affinity and high capacity, GLUT2 transports dietary sugars, glucose, fructose and galactose in a large range of physiologica...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章,评审

    doi:10.1007/BF03168378

    authors: Leturque A,Brot-Laroche E,Le Gall M,Stolarczyk E,Tobin V

    更新日期:2005-12-01 00:00:00

  • Simvastatin and vitamin E effects on cardiac and hepatic oxidative stress in rats fed on high fat diet.

    abstract::High fat diet (HFD) is a common cause of metabolic syndrome and type 2 diabetes mellitus. Published data showed that HFD and subsequent dyslipidemia are major triggers for oxidative stress. Forty-eight male Sprague-Dawley rats, weighing 170-200 g, were divided into six groups: control, control with vitamin E (100 mg/k...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s13105-013-0250-y

    authors: Abbas AM,Sakr HF

    更新日期:2013-12-01 00:00:00

  • Critical difference applied to exercise-induced salivary testosterone and cortisol using enzyme-linked immunosorbent assay (ELISA): distinguishing biological from statistical change.

    abstract::Due to its noninvasive, convenient, and practical nature, salivary testosterone (sal-T) and cortisol (sal-C) are frequently used in a clinical and applied setting. However, few studies report biological and analytical error and even fewer report the 'critical difference' which is the change required before a true biol...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s13105-014-0368-6

    authors: Hayes LD,Sculthorpe N,Young JD,Baker JS,Grace FM

    更新日期:2014-12-01 00:00:00

  • Regulation of the O-acetyl-L-serine(thiol)lyase activity in Monoraphidium braunii.

    abstract::Total level of O-acetyl-L-serine(thiol)lyase (OASTL) activity observed in Monoraphidium braunii fed-repleted cells decreases up to 40% after 24 h the carbon source was removed from the culture; however, no significant change in the activity is observed in N-starved cells. On the other hand, sulfur starvation induces O...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:

    authors: González-Arroyo JG,Vega JM,Pérez-Castiñeira JR

    更新日期:1998-09-01 00:00:00

  • Targeting of histamine producing cells by EGCG: a green dart against inflammation?

    abstract::The human body is made of some 250 different cell types. From them, only a small subset of cell types is able to produce histamine. They include some neurons, enterochromaffin-like cells, gastrin-containing cells, mast cells, basophils, and monocytes/macrophages, among others. In spite of the reduced number of these h...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章,评审

    doi:10.1007/s13105-010-0033-7

    authors: Melgarejo E,Medina MA,Sánchez-Jiménez F,Urdiales JL

    更新日期:2010-09-01 00:00:00

  • Protein kinase C cross-talk with gonadotrope progesterone receptor is involved in GnRH-induced LH secretion.

    abstract::In the absence of progesterone (P), the anti-P at the receptor RU486 reduces basal and GnRH-stimulated LH secretion both in vivo and in vitro, demonstrating the existence of a ligand-independent activation of progesterone receptor (LIAPR). The aim of the present study was to determine which component of the intracellu...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/BF03165804

    authors: Garrido-Gracia JC,Bellido C,Aguilar R,Sánchez-Criado JE

    更新日期:2006-03-01 00:00:00

  • Anthropometric indexes for visceral fat estimation in overweight/obese women attending to age and menopausal status.

    abstract::The objective of the present study was to assess the usefulness and accuracy of different anthropometric measurements in the diagnosis of abdominal visceral obesity in overweight/obese women attending to age and menopausal status. The secondary objective was to evaluate the usefulness of waist circumference (WC) in tw...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/BF03165753

    authors: Garaulet M,Hernández-Morante JJ,Tébar FJ,Zamora S

    更新日期:2006-12-01 00:00:00

  • About the controversies of the cardioprotective effect of n-3 polyunsaturated fatty acids (PUFAs) between animal studies and clinical meta-analyses: a review with several strategies to enhance the beneficial effects of n-3 PUFAs.

    abstract::Several meta-analyses describing the effect of n-3 polyunsaturated fatty acids on the survival rate of the victims of an acute coronary event do not clearly support a beneficial impact of these fatty acids. Yet, animal studies consistently show n-3 PUFA-induced protection against ischemia-reperfusion-induced myocardia...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章,评审

    doi:10.1007/s13105-019-00670-y

    authors: Demaison L,Leger T,Vergely C,Rochette L,Azarnoush K

    更新日期:2019-08-01 00:00:00

  • Effects of different doses of resveratrol on body fat and serum parameters in rats fed a hypercaloric diet.

    abstract::Recently resveratrol, a compound naturally occurring in various plants, has been proposed as a potential anti-obesity compound. The aim of the present work was to analyse the effects of different doses of resveratrol on body fat and serum parameters in rats. Thirty-two male Sprague-Dawley rats were randomly divided in...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/BF03185932

    authors: Macarulla MT,Alberdi G,Gómez S,Tueros I,Bald C,Rodríguez VM,Martínez JA,Portillo MP

    更新日期:2009-12-01 00:00:00

  • Effects of overexpression of growth hormone on T cell activity in transgenic mice.

    abstract::Growth hormone plays a key role in the maturation and maintenance of the immune response, however, the effects of chronic high circulating concentrations of the hormone on the immune system is poorly understood. Transgenic mice overexpressing bovine growth hormone (b-GH) gene, fused to the rat phosphoenolpyruvate carb...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/BF03179853

    authors: Esquifino AI,Arce A,Alvarez MP,Szary A,Brown-Borg H,Bartke A

    更新日期:2002-09-01 00:00:00

  • The protective microRNA-199a-5p-mediated unfolded protein response in hypoxic cardiomyocytes is regulated by STAT3 pathway.

    abstract::The protective effects of downregulated miR-199a-5p on ischemic and hypoxic cardiomyocytes were well recognized, but the underlying mechanism of inhibited miR-199a-5p is not yet clear. The present study explored the relationship between enhanced signal transducer and activator of transcription 3 (STAT3) signaling and ...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s13105-018-0657-6

    authors: Zhou Y,Pang B,Xiao Y,Zhou S,He B,Zhang F,Liu W,Peng H,Li P

    更新日期:2019-02-01 00:00:00

  • Role of alpha- and beta-adrenoreceptors in rat monocyte/macrophage function at rest and acute exercise.

    abstract::Previous studies from our laboratory have demonstrated that a single bout of moderate exercise stimulates macrophage function, increasing phagocytic capacity, and production of hydrogen peroxide and nitric oxide (NO˙) through nuclear factor kappa B activation. In this work, we investigated the role of α- and β-adrenor...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s13105-013-0310-3

    authors: da Silva Rossato J,Krause M,Fernandes AJ,Fernandes JR,Seibt IL,Rech A,Homem de Bittencourt PI Jr

    更新日期:2014-06-01 00:00:00

  • Gene methylation parallelisms between peripheral blood cells and oral mucosa samples in relation to overweight.

    abstract::Epigenetics has an important role in the regulation of metabolic adaptation to environmental modifications. In this sense, the determination of epigenetic changes in non-invasive samples during the development of metabolic diseases could play an important role in the procedures in primary healthcare practice. To help ...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s13105-017-0560-6

    authors: San-Cristobal R,Navas-Carretero S,Milagro FI,Riezu-Boj JI,Guruceaga E,Celis-Morales C,Livingstone KM,Brennan L,Lovegrove JA,Daniel H,Saris WH,Traczyk I,Manios Y,Gibney ER,Gibney MJ,Mathers JC,Martinez JA

    更新日期:2016-08-01 00:00:00

  • Double-stranded Let-7 mimics, potential candidates for cancer gene therapy.

    abstract::MicroRNAs (miRNAs), a class of small, single-stranded endogenous RNAs, act as post-transcriptional regulators of gene expression. The ability of one single miRNA regulating multiple functionally related mRNAs makes it a new potential candidate for cancer gene therapy. Let-7s miRNAs have been demonstrated as tumor-supp...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s13105-011-0124-0

    authors: Wang QZ,Lv YH,Gong YH,Li ZF,Xu W,Diao Y,Xu R

    更新日期:2012-03-01 00:00:00

  • Protein complex immunological separation assay (ProCISA): a technique for investigating single protein properties.

    abstract::Analysis of the posttranslational modification of proteins, such as phosphorylation, might yield misleading results due to the presence of other proteins with similar electrophoretic properties that coimmunoprecipitate with the target protein. The aim of the present work was to develop a reliable, easy and economical ...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/BF03178839

    authors: Redondo PC,Rosado JA,Salido GM,Sage SO

    更新日期:2008-09-01 00:00:00

  • Effects of eicosapentaenoic acid (EPA) on adiponectin gene expression and secretion in primary cultured rat adipocytes.

    abstract::Adiponectin, a hormone produced by adipocytes, is involved in glucose metabolism and insulin sensitivity. The production of this adipokine is impaired in obesity and insulin resistance. Eicosapentaenoic acid (EPA) is a dietary n-3 polyunsaturated fatty acid that improves insulin sensitivity in several models of obesit...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/BF03174067

    authors: Lorente-Cebrián S,Pérez-Matute P,Martínez JA,Marti A,Moreno-Aliaga MJ

    更新日期:2006-06-01 00:00:00

  • RARgamma and TRbeta expressions are decreased in PBMC and SWAT of obese subjects in weight gain.

    abstract::In order to evaluate the expression of nuclear receptors at the peripheral level in obese subjects, messenger RNA (mRNA) levels of different isoforms of retinoic acid receptor (RAR), triiodothyronine (TR), and peroxisome proliferator-activated receptor (PPAR) were determined and compared in peripheral mononuclear bloo...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s13105-010-0006-x

    authors: Bairras C,Redonnet A,Dabadie H,Gin H,Atgie C,Pallet V,Higueret P,Noël-Suberville C

    更新日期:2010-03-01 00:00:00

  • Glycemia and insulinemia evaluation after high-sucrose and high-fat diets in lean and overweight/obese women.

    abstract::The study investigates the effect of weight-maintaining high-sucrose (HSD) and high-fat (HFD) diets on plasma glucose and insulin concentrations in lean and obese women, and verifies the correlation between insulin profile and body composition. Lean (G1 group, n=6, BMI= 21.4 (20.2-22.8) kg/m2) and overweight/obese (G2...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/BF03168238

    authors: Volp AC,Hermsdorff HH,Bressan J

    更新日期:2008-06-01 00:00:00

  • Protein expression changed by nicotine in rat vascular smooth muscle cells.

    abstract::In order to observe the effects of nicotine on protein expression in rat vascular smooth muscle cells (SMCs), nicotine treated SMCs were studied by proteomic technologies combining two-dimensional electrophoresis (2-DE) and peptide mass fingerprinting (PMF). Real-time RT-PCR was used to validate the differentially exp...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/BF03168227

    authors: Wang Z,Wu W,Fang X,Wang Y,Xiao C,Zhao R,Wang L,Qiao Z

    更新日期:2007-06-01 00:00:00

  • Influence of exercise on NA- and Hsp72-induced release of IFNγ by the peritoneal suspension of macrophages and lymphocytes from genetically obese Zucker rats.

    abstract::Regular physical exercise is recognized as a nonpharmacological therapeutic strategy in the treatment of metabolic syndrome, and has been proposed for improving obesity, diabetic status, insulin resistance, and immune response. The aim of the present study was to evaluate the effect of a regular exercise program (trea...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s13105-012-0196-5

    authors: Martín-Cordero L,García JJ,Hinchado MD,Bote E,Ortega E

    更新日期:2013-03-01 00:00:00

  • Influence of chronic ethanol consumption on the muscarinic cholinergic control of rat pancreatic acinar cells.

    abstract::There are a number of hypothetical explanations for the actions of ethanol on the exocrine pancreas; among them, the cholinergic hypothesis has received special attention. According to this hypothesis, chronic alcohol consumption induces alterations in the control of exocrine pancreatic function resulting in cholinerg...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/BF03179781

    authors: Acosta JJ,San Román JI,López MA,Calvo JJ

    更新日期:2000-09-01 00:00:00

  • Effects of lipoic acid on apelin in 3T3-L1 adipocytes and in high-fat fed rats.

    abstract::Lipoic acid (LA) is an antioxidant with therapeutic properties on several diseases like diabetes and obesity. Apelin is a novel adipokine with potential beneficial actions on glucose metabolism and insulin resistance. The aim of this study was to examine in 3T3-L1 adipocytes the effects of LA on apelin gene expression...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s13105-011-0087-1

    authors: Fernández-Galilea M,Pérez-Matute P,Prieto-Hontoria P,Martínez JA,Moreno-Aliaga MJ

    更新日期:2011-09-01 00:00:00

  • Effects of orange and apple pectin on cholesterol concentration in serum, liver and faeces.

    abstract::To investigate the effects of pectin on cholesterol metabolism, normal rats were fed for three weeks a diet containing 2.5 or 5 % apple or orange pectin, or without pectin (control). Cholesterol concentrations were determined in faeces after 1, 2 and 3 weeks of treatment, and in liver and serum at the end of the exper...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:

    authors: González M,Rivas C,Caride B,Lamas MA,Taboada MC

    更新日期:1998-06-01 00:00:00

  • Hypophagia and induction of serotonin transporter gene expression in raphe nuclei of male and female rats after short-term fluoxetine treatment.

    abstract::Serotonin (5-HT) is one of the regulators of feeding in humans. Drugs acting on the serotoninergic system are used to treat bulimia nervosa and to enhance the effect of hypocaloric diets in overweight subjects. They act rapidly to normalise feeding when used to treat eating-related problems. To explore the role of the...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s13105-012-0188-5

    authors: Lauzurica N,García-García L,Fuentes JA,Delgado M

    更新日期:2013-03-01 00:00:00

  • Physiological adaptations to exercise in people with spinal cord injury.

    abstract::The number of patients that suffer some type of spinal cord lesion in recent years are high and have increased because of factors such as traffic accidents. Although their life expectancy has increased, cardiovascular illnesses is one of the main causes of morbidity and mortality. Since the degree of physical fitness ...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/BF03179863

    authors: Vidal J,Javierre C,Segura R,Lizarraga A,Barbany JR,Pérez A

    更新日期:2003-03-01 00:00:00

  • Glucose and insulin modify thrombospondin 1 expression and secretion in primary adipocytes from diet-induced obese rats.

    abstract::Thrombospondin 1 (TSP-1), an antiangiogenic factor and transforming growth factor (TGF)-β activity regulator, has been recently recognized as an adipokine that correlates with obesity, inflammation and insulin resistance processes. In the present study, epididymal adipocytes of rats that were fed a chow or a high-fat ...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s13105-011-0081-7

    authors: Garcia-Diaz DF,Arellano AV,Milagro FI,Moreno-Aliaga MJ,Portillo MP,Martinez JA,Campion J

    更新日期:2011-09-01 00:00:00

  • Does nitric oxide play a role in maternal tolerance towards the foetus?

    abstract::In pregnancy there occurs maternal tolerance to the foetus. Several mechanisms have been proposed to explain this phenomenon. The main immune population in the decidua are macrophages and natural killer cells, but with some "special" suppressor characteristics. There is also a predominant TH2 response. The non classic...

    journal_title:Journal of physiology and biochemistry

    pub_type: 杂志文章,评审

    doi:10.1007/BF03167033

    authors: González A,López AS,Alegre E,Alcázar JL,López-Moratalla N

    更新日期:2004-09-01 00:00:00