Abstract：
:MicroRNAs (miRNAs), a class of small, single-stranded endogenous RNAs, act as post-transcriptional regulators of gene expression. The ability of one single miRNA regulating multiple functionally related mRNAs makes it a new potential candidate for cancer gene therapy. Let-7s miRNAs have been demonstrated as tumor-suppressor genes in various types of cancers, providing one choice of gene therapy by replenishing this miRNA. In the present studies, we demonstrate that the chemically synthesized, double-stranded Let-7 mimics can inhibit the growth and migration and induce the cell cycle arrest of lung cancer cell lines in vitro. Let-7 mimics silence gene expression by binding to the 3' UTR of targeting mRNAs. Mutation of seed sequence significantly depresses the gene silencing activity of Let-7 mimics. Our results also demonstrate that it is possible to increase the activity of Let-7s through mutating the sequence within the 3'end of the antisense strand. Directly, co-transfection Let-7 mimics with active siRNAs impairs the anti-cancer activities of Let-7 mimics. However, a 3-h interval between the introduction of Let-7 mimics and a kind of siRNA avoids the competition and enhances the anti-cancer activities of Let-7 mimics. Taken together, these results have revealed that Let-7s mimics are potential candidates for cancer gene therapy.
journal_name
J Physiol Biochemjournal_title
Journal of physiology and biochemistryauthors
Wang QZ,Lv YH,Gong YH,Li ZF,Xu W,Diao Y,Xu Rdoi
10.1007/s13105-011-0124-0subject
Has Abstractpub_date
2012-03-01 00:00:00pages
107-19issue
1eissn
1138-7548issn
1877-8755journal_volume
68pub_type
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journal_title：Journal of physiology and biochemistry
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pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
doi：10.1007/s13105-013-0265-4
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journal_title：Journal of physiology and biochemistry
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journal_title：Journal of physiology and biochemistry
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF03180578
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journal_title：Journal of physiology and biochemistry
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pub_type： 杂志文章
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
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更新日期：2016-06-01 00:00:00
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journal_title：Journal of physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s13105-016-0518-0
更新日期：2016-12-01 00:00:00
