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Comparative evaluation of four serodiagnostic tests for scrub typhus in Thailand.

Abstract：

:The commercial dot-blot enzyme-linked immunosorbent assay Dip-S-Ticks dipstick test was compared with the indirect immunoperoxidase (IIP) and Weil-Felix (WF) tests for the diagnosis of scrub typhus, using the indirect immunofluorescent antibody test (IFA) as the reference standard. With a panel of 117 positive and 75 negative sera, the dipstick test was 94% sensitive and 98.7% specific at a cut-off value of one or more positive dots. The IIP was 90.6% sensitive and 100% specific at a cut-off titre of 1:400, and was more sensitive than the IFA with acute sera (79.6% vs. 68.5% at a titre > or = 1:400). All 3 were superior to the WF, which lacked sensitivity. The dipstick assay was easy to perform and did not require sophisticated electrical equipment, and the results were available within one hour. It is therefore suitable for use in rural Thailand, where scrub typhus is common.

journal_name

Trans R Soc Trop Med Hyg

journal_title

Transactions of the Royal Society of Tropical Medicine and Hygiene

authors

Pradutkanchana J,Silpapojakul K,Paxton H,Pradutkanchana S,Kelly DJ,Strickman D

doi

10.1016/s0035-9203(97)90266-2

subject

Has Abstract

pub_date

1997-07-01 00:00:00

pages

425-8

issue

4

eissn

0035-9203

issn

1878-3503

journal_volume

91

pub_type

杂志文章

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